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Experimental study on anti-inflammatory activity of a TCM recipe consisting of the supercritical fluid CO2 extract of Chrysanthemum indicum, Patchouli Oil and Zedoary Turmeric Oil in vivo.
J Ethnopharmacol. 2012 Jun 01; 141(2):608-14.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Chrysanthemum indicum (Compositae) Linné, Pogostemon cablin (Blanco) Benth and Curcuma wenyujin (Zingiberaceae) Y. H. Chen et C. Ling are three of the extensively used herbal remedies among traditional Chinese medicines for the purpose of anti-inflammation. A traditional Chinese medicine (TCM) recipe named CPZ consisting extracts of the above three herbs, has shown noteworthy anti-influenza activity, which is closely related to its anti-inflammatory feature.

AIM OF THIS STUDY

To investigated the anti-inflammtory activity of CPZ in vivo for a further exploration of the recipe's anti-inflammatory properties.

MATERIALS AND METHODS

The anti-inflammatory property of CPZ on acute inflammation was evaluated by inflammatory models of dimethylbenzene (DMB)-induced ear vasodilatation and acetic acid-induced capillary permeability enhancement in mice, as well as the carrageenan-induced paw edema rat model, in which inflammation-related cytokine including prostaglandin E(2) (PGE(2)), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and nitric oxide (NO) in the edematous paw tissue were determined by enzyme linked immunosorbent assay (ELISA). Moreover, effect of CPZ on chronic inflammation was observed through granuloma formation in rats subjected to cotton pellet implantation.

RESULTS

CPZ (340, 170, and 85 mg/kg for mice, p.o.) not only decreased the DMB-induced ear vasodilatation but also attenuated capillary permeability under acetic acid challenge in mice. And the significant inhibition on carrageenan-induced paw edema was observed. Further more, the ELISA results showed that CPZ (170, 85, and 42.5 mg/kg for rats, p.o.) could up-regulate the level of IL-1β in the edema paw tissue of rats significantly while down-regulate that of PGE(2), but no apparent effect on TNF-α or NO was observed in the test. Besides, CPZ had a certain degree of restraining effect on the cotton pellet-induced granuloma formation in rats and the highest dose of 170 mg/kg even showed a significant suppression on it.

CONCLUSION

The above results indicated that CPZ possessed a potent anti-inflammatory activity, which is indicated to be closely associated with its regulation on IL-1β and PGE(2) thereby mediating the inflammatory response acting at an appropriate level.

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Authors+Show Affiliations

Su JY
Dongguan Mathematical Engineering Academy of Chinese Medicine, Guangzhou University of Chinese Medicine, Dongguan 523808, China.
Tan LR
No affiliation info available
Lai P
No affiliation info available
Liang HC
No affiliation info available
Qin Z
No affiliation info available
Ye MR
No affiliation info available
Lai XP
No affiliation info available
Su ZR
No affiliation info available

MeSH

Acetic AcidAnimalsAnti-Inflammatory AgentsCapillary PermeabilityCarbon DioxideCarrageenanChromatography, Supercritical FluidDinoprostoneDisease Models, AnimalDose-Response Relationship, DrugDrugs, Chinese HerbalEdemaEnzyme-Linked Immunosorbent AssayInflammationInflammation MediatorsInterleukin-1betaMedicine, Chinese TraditionalMiceNitric OxidePlants, MedicinalRatsRats, Sprague-DawleySolventsTime FactorsTumor Necrosis Factor-alphaVasodilationXylenes

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21920423

Citation

Su, Ji-Yan, et al. "Experimental Study On Anti-inflammatory Activity of a TCM Recipe Consisting of the Supercritical Fluid CO2 Extract of Chrysanthemum Indicum, Patchouli Oil and Zedoary Turmeric Oil in Vivo." Journal of Ethnopharmacology, vol. 141, no. 2, 2012, pp. 608-14.
Su JY, Tan LR, Lai P, et al. Experimental study on anti-inflammatory activity of a TCM recipe consisting of the supercritical fluid CO2 extract of Chrysanthemum indicum, Patchouli Oil and Zedoary Turmeric Oil in vivo. J Ethnopharmacol. 2012;141(2):608-14.
Su, J. Y., Tan, L. R., Lai, P., Liang, H. C., Qin, Z., Ye, M. R., Lai, X. P., & Su, Z. R. (2012). Experimental study on anti-inflammatory activity of a TCM recipe consisting of the supercritical fluid CO2 extract of Chrysanthemum indicum, Patchouli Oil and Zedoary Turmeric Oil in vivo. Journal of Ethnopharmacology, 141(2), 608-14. https://doi.org/10.1016/j.jep.2011.08.055
Su JY, et al. Experimental Study On Anti-inflammatory Activity of a TCM Recipe Consisting of the Supercritical Fluid CO2 Extract of Chrysanthemum Indicum, Patchouli Oil and Zedoary Turmeric Oil in Vivo. J Ethnopharmacol. 2012 Jun 1;141(2):608-14. PubMed PMID: 21920423.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Experimental study on anti-inflammatory activity of a TCM recipe consisting of the supercritical fluid CO2 extract of Chrysanthemum indicum, Patchouli Oil and Zedoary Turmeric Oil in vivo. AU - Su,Ji-Yan, AU - Tan,Li-Rong, AU - Lai,Ping, AU - Liang,Hai-Chun, AU - Qin,Zhen, AU - Ye,Mu-Rong, AU - Lai,Xiao-Ping, AU - Su,Zi-Ren, Y1 - 2011/09/03/ PY - 2011/06/18/received PY - 2011/08/23/revised PY - 2011/08/25/accepted PY - 2011/9/17/entrez PY - 2011/9/17/pubmed PY - 2012/9/29/medline SP - 608 EP - 14 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 141 IS - 2 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Chrysanthemum indicum (Compositae) Linné, Pogostemon cablin (Blanco) Benth and Curcuma wenyujin (Zingiberaceae) Y. H. Chen et C. Ling are three of the extensively used herbal remedies among traditional Chinese medicines for the purpose of anti-inflammation. A traditional Chinese medicine (TCM) recipe named CPZ consisting extracts of the above three herbs, has shown noteworthy anti-influenza activity, which is closely related to its anti-inflammatory feature. AIM OF THIS STUDY: To investigated the anti-inflammtory activity of CPZ in vivo for a further exploration of the recipe's anti-inflammatory properties. MATERIALS AND METHODS: The anti-inflammatory property of CPZ on acute inflammation was evaluated by inflammatory models of dimethylbenzene (DMB)-induced ear vasodilatation and acetic acid-induced capillary permeability enhancement in mice, as well as the carrageenan-induced paw edema rat model, in which inflammation-related cytokine including prostaglandin E(2) (PGE(2)), interleukin-1β (IL-1β), tumor necrosis factor-α (TNF-α), and nitric oxide (NO) in the edematous paw tissue were determined by enzyme linked immunosorbent assay (ELISA). Moreover, effect of CPZ on chronic inflammation was observed through granuloma formation in rats subjected to cotton pellet implantation. RESULTS: CPZ (340, 170, and 85 mg/kg for mice, p.o.) not only decreased the DMB-induced ear vasodilatation but also attenuated capillary permeability under acetic acid challenge in mice. And the significant inhibition on carrageenan-induced paw edema was observed. Further more, the ELISA results showed that CPZ (170, 85, and 42.5 mg/kg for rats, p.o.) could up-regulate the level of IL-1β in the edema paw tissue of rats significantly while down-regulate that of PGE(2), but no apparent effect on TNF-α or NO was observed in the test. Besides, CPZ had a certain degree of restraining effect on the cotton pellet-induced granuloma formation in rats and the highest dose of 170 mg/kg even showed a significant suppression on it. CONCLUSION: The above results indicated that CPZ possessed a potent anti-inflammatory activity, which is indicated to be closely associated with its regulation on IL-1β and PGE(2) thereby mediating the inflammatory response acting at an appropriate level. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21920423/Experimental_study_on_anti_inflammatory_activity_of_a_TCM_recipe_consisting_of_the_supercritical_fluid_CO2_extract_of_Chrysanthemum_indicum_Patchouli_Oil_and_Zedoary_Turmeric_Oil_in_vivo_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00628-3 DB - PRIME DP - Unbound Medicine ER -