Tags

Type your tag names separated by a space and hit enter

Experimental model of degloving injury in rats: effect of allopurinol and pentoxifylline in improving viability of avulsed flaps.
Ann Plast Surg. 2013 Mar; 70(3):366-9.AP

Abstract

BACKGROUND

Degloving injuries may be a challenge when it comes to deciding the surgical approach to be used. Repositioning of the flap and suturing are faster and more straightforward, but often these procedures often lead to total or partial loss of the avulsed flap. Pharmacological agents with vascular properties that enhance the viability of the reattached flap could be beneficial to patients with degloving injuries. Experimental models with which to test this hypothesis are scarce. An experimental model reproducing a degloving injury of the hind limb of rats was developed in our department, and the effects of pentoxifylline (Ptx) and allopurinol (Alp) were assessed.

METHODS

In all, 3 groups of rats were studied (25 rats each). A hind limb degloving model was used in all groups, resulting in a reverse flow flap. The flap was then repositioned and sutured. The control (Ct) group received only saline solution, the Ptx group received pentoxifylline (25 mg/kg), and the Alp group received allopurinol (45 mg/kg). The rats were observed for 7 days, after which they were killed, and the flap was removed. The total area of the avulsed flap and the necrotic area were measured.

RESULTS

The median total flap area (cm) was 5.6 for the Ct group, 5.5 for the Ptx group, and 5.8 for the Alp group (P = 0.9465). Thus, the flaps were similar. The median necrotic flap area (cm) was 3.3 for the Ct group, 2.3 for the Ptx group, and 1.9 for the Alp group (P = 0.0001). There was a statistical difference between the Ct and Ptx groups and the Ct and Alp groups (P < 0.05).

CONCLUSIONS

The areas of necrosis observed in the degloved flaps of the rats' hind limbs were smaller in the pentoxifylline and allopurinol groups. Although allopurinol seems to be more efficient, the difference was not significant.

Authors+Show Affiliations

Division of Plastic Surgery, University of São Paulo, São Paulo, Brazil. drdimasandre@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21921788

Citation

Milcheski, Dimas A., et al. "Experimental Model of Degloving Injury in Rats: Effect of Allopurinol and Pentoxifylline in Improving Viability of Avulsed Flaps." Annals of Plastic Surgery, vol. 70, no. 3, 2013, pp. 366-9.
Milcheski DA, Nakamoto HA, Tuma P, et al. Experimental model of degloving injury in rats: effect of allopurinol and pentoxifylline in improving viability of avulsed flaps. Ann Plast Surg. 2013;70(3):366-9.
Milcheski, D. A., Nakamoto, H. A., Tuma, P., Nóbrega, L., & Ferreira, M. C. (2013). Experimental model of degloving injury in rats: effect of allopurinol and pentoxifylline in improving viability of avulsed flaps. Annals of Plastic Surgery, 70(3), 366-9. https://doi.org/10.1097/SAP.0b013e318230601a
Milcheski DA, et al. Experimental Model of Degloving Injury in Rats: Effect of Allopurinol and Pentoxifylline in Improving Viability of Avulsed Flaps. Ann Plast Surg. 2013;70(3):366-9. PubMed PMID: 21921788.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Experimental model of degloving injury in rats: effect of allopurinol and pentoxifylline in improving viability of avulsed flaps. AU - Milcheski,Dimas A, AU - Nakamoto,Hugo A, AU - Tuma,Paulo,Jr AU - Nóbrega,Lucas, AU - Ferreira,Marcus C, PY - 2011/9/17/entrez PY - 2011/9/17/pubmed PY - 2013/6/12/medline SP - 366 EP - 9 JF - Annals of plastic surgery JO - Ann Plast Surg VL - 70 IS - 3 N2 - BACKGROUND: Degloving injuries may be a challenge when it comes to deciding the surgical approach to be used. Repositioning of the flap and suturing are faster and more straightforward, but often these procedures often lead to total or partial loss of the avulsed flap. Pharmacological agents with vascular properties that enhance the viability of the reattached flap could be beneficial to patients with degloving injuries. Experimental models with which to test this hypothesis are scarce. An experimental model reproducing a degloving injury of the hind limb of rats was developed in our department, and the effects of pentoxifylline (Ptx) and allopurinol (Alp) were assessed. METHODS: In all, 3 groups of rats were studied (25 rats each). A hind limb degloving model was used in all groups, resulting in a reverse flow flap. The flap was then repositioned and sutured. The control (Ct) group received only saline solution, the Ptx group received pentoxifylline (25 mg/kg), and the Alp group received allopurinol (45 mg/kg). The rats were observed for 7 days, after which they were killed, and the flap was removed. The total area of the avulsed flap and the necrotic area were measured. RESULTS: The median total flap area (cm) was 5.6 for the Ct group, 5.5 for the Ptx group, and 5.8 for the Alp group (P = 0.9465). Thus, the flaps were similar. The median necrotic flap area (cm) was 3.3 for the Ct group, 2.3 for the Ptx group, and 1.9 for the Alp group (P = 0.0001). There was a statistical difference between the Ct and Ptx groups and the Ct and Alp groups (P < 0.05). CONCLUSIONS: The areas of necrosis observed in the degloved flaps of the rats' hind limbs were smaller in the pentoxifylline and allopurinol groups. Although allopurinol seems to be more efficient, the difference was not significant. SN - 1536-3708 UR - https://www.unboundmedicine.com/medline/citation/21921788/Experimental_model_of_degloving_injury_in_rats:_effect_of_allopurinol_and_pentoxifylline_in_improving_viability_of_avulsed_flaps_ L2 - http://dx.doi.org/10.1097/SAP.0b013e318230601a DB - PRIME DP - Unbound Medicine ER -