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Reactive oxygen species-mediated mitochondrial dysfunction is involved in apoptosis in human nasopharyngeal carcinoma CNE cells induced by Selaginella doederleinii extract.
J Ethnopharmacol 2011; 138(1):184-91JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

A traditional Chinese medicine Selaginella doederleinii Hieron has been combined with radiotherapy for the treatment of human nasopharyngeal carcinoma in clinic in China. However, the detailed mechanism of anti-tumor effect of Selaginella doederleinii remains elusive.

AIM OF THE STUDY

This study was designed to investigate the anti-tumor effect of ethanol extract of Selaginella doederleinii (SDE) on human nasopharyngeal carcinoma and its possible mechanisms.

MATERIALS AND METHODS

Viability, apoptosis and protein expression of tumor cells were analyzed by MTT, Annexin V staining and Western blot, respectively. Formation of intracellular reactive oxygen species was determined using dichlorofluorescin fluorescence. The in vivo anti-tumor effect was evaluated by measuring tumor volume changes and TUNEL staining in nude mice.

RESULTS

SDE significantly inhibited the growth and induced apoptosis in human nasopharyngeal carcinoma CNE cells. In addition, SDE triggered the mitochondrial/caspase apoptotic pathway indicated by enhanced Bax-to-Bcl-2 ratio, loss of mitochondrial membrane potential, cytochrome c release, and caspase cascade. Moreover, SDE provoked the generation of reactive oxygen species in CNE cells, while the antioxidant N-acetyl cysteine almost completely blocked SDE-induced disruption of mitochondrial membrane potential, caspases activation and apoptosis. Furthermore, a transplantable nude mice model was utilized to estimate the effectiveness of SDE in vivo. The treated mice displayed decreased tumor size, which was associated with enhanced apoptotic cell death.

CONCLUSIONS

These results, offering solid evidence of the induction of mitochondria-related apoptosis in tumor cells, provide the molecular theoretical basis of clinical application of Selaginella doederleinii for the treatment of human nasopharyngeal carcinoma.

Authors+Show Affiliations

Department of Otolaryngology - Head and Neck Surgery, Changzheng Hospital, Second Military Medical University, 415 Fengyang Road, Shanghai 200433, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21924341

Citation

Liu, Huanhai, et al. "Reactive Oxygen Species-mediated Mitochondrial Dysfunction Is Involved in Apoptosis in Human Nasopharyngeal Carcinoma CNE Cells Induced By Selaginella Doederleinii Extract." Journal of Ethnopharmacology, vol. 138, no. 1, 2011, pp. 184-91.
Liu H, Peng H, Ji Z, et al. Reactive oxygen species-mediated mitochondrial dysfunction is involved in apoptosis in human nasopharyngeal carcinoma CNE cells induced by Selaginella doederleinii extract. J Ethnopharmacol. 2011;138(1):184-91.
Liu, H., Peng, H., Ji, Z., Zhao, S., Zhang, Y., Wu, J., ... Liao, J. (2011). Reactive oxygen species-mediated mitochondrial dysfunction is involved in apoptosis in human nasopharyngeal carcinoma CNE cells induced by Selaginella doederleinii extract. Journal of Ethnopharmacology, 138(1), pp. 184-91. doi:10.1016/j.jep.2011.08.072.
Liu H, et al. Reactive Oxygen Species-mediated Mitochondrial Dysfunction Is Involved in Apoptosis in Human Nasopharyngeal Carcinoma CNE Cells Induced By Selaginella Doederleinii Extract. J Ethnopharmacol. 2011 Oct 31;138(1):184-91. PubMed PMID: 21924341.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Reactive oxygen species-mediated mitochondrial dysfunction is involved in apoptosis in human nasopharyngeal carcinoma CNE cells induced by Selaginella doederleinii extract. AU - Liu,Huanhai, AU - Peng,Hu, AU - Ji,Zhenhua, AU - Zhao,Shuwei, AU - Zhang,Yinfang, AU - Wu,Jian, AU - Fan,Jingping, AU - Liao,Jianchun, Y1 - 2011/09/08/ PY - 2011/04/27/received PY - 2011/07/29/revised PY - 2011/08/31/accepted PY - 2011/9/20/entrez PY - 2011/9/20/pubmed PY - 2012/5/4/medline SP - 184 EP - 91 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 138 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: A traditional Chinese medicine Selaginella doederleinii Hieron has been combined with radiotherapy for the treatment of human nasopharyngeal carcinoma in clinic in China. However, the detailed mechanism of anti-tumor effect of Selaginella doederleinii remains elusive. AIM OF THE STUDY: This study was designed to investigate the anti-tumor effect of ethanol extract of Selaginella doederleinii (SDE) on human nasopharyngeal carcinoma and its possible mechanisms. MATERIALS AND METHODS: Viability, apoptosis and protein expression of tumor cells were analyzed by MTT, Annexin V staining and Western blot, respectively. Formation of intracellular reactive oxygen species was determined using dichlorofluorescin fluorescence. The in vivo anti-tumor effect was evaluated by measuring tumor volume changes and TUNEL staining in nude mice. RESULTS: SDE significantly inhibited the growth and induced apoptosis in human nasopharyngeal carcinoma CNE cells. In addition, SDE triggered the mitochondrial/caspase apoptotic pathway indicated by enhanced Bax-to-Bcl-2 ratio, loss of mitochondrial membrane potential, cytochrome c release, and caspase cascade. Moreover, SDE provoked the generation of reactive oxygen species in CNE cells, while the antioxidant N-acetyl cysteine almost completely blocked SDE-induced disruption of mitochondrial membrane potential, caspases activation and apoptosis. Furthermore, a transplantable nude mice model was utilized to estimate the effectiveness of SDE in vivo. The treated mice displayed decreased tumor size, which was associated with enhanced apoptotic cell death. CONCLUSIONS: These results, offering solid evidence of the induction of mitochondria-related apoptosis in tumor cells, provide the molecular theoretical basis of clinical application of Selaginella doederleinii for the treatment of human nasopharyngeal carcinoma. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/21924341/Reactive_oxygen_species_mediated_mitochondrial_dysfunction_is_involved_in_apoptosis_in_human_nasopharyngeal_carcinoma_CNE_cells_induced_by_Selaginella_doederleinii_extract_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00645-3 DB - PRIME DP - Unbound Medicine ER -