Tags

Type your tag names separated by a space and hit enter

Ameliorative effects of curcumin against renal injuries mediated by inducible nitric oxide synthase and nuclear factor kappa B during gentamicin-induced toxicity in Wistar rats.
Eur J Pharmacol. 2011 Nov 30; 670(2-3):578-85.EJ

Abstract

The ameliorative role of curcumin in attenuating gentamicin-induced nephrotoxicity has been reported earlier however, the mechanism of action remains unclear. Gentamicin was injected intraperitoneally (100 mg/kg body weight) once daily for 6 days. Curcumin was administered orally (200 mg/kg body weight) once daily for 7, 15 and 30 days. Gentamicin-induced rats showed significant increase in the levels of kidney markers and the activities of urinary marker enzymes, which was reversed upon curcumin treatment. A significant increase in kidney lipid peroxidation (LPO) and decrease in activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and reduced glutathione (GSH) were observed in gentamicin-induced rats. Immunohistochemical, Western blot and RT-PCR studies in gentamicin-induced rats also demonstrated an increase in the levels of inducible nitric oxide synthase (iNOS) and nuclear factor-κB (NF-κB). All these effects induced by gentamicin were reduced upon treatment with curcumin in a time dependent manner. To conclude, curcumin enhances antioxidants, and decreases iNOS and NF-κB, thereby protecting the cells against oxidative stress induced by gentamicin.

Authors+Show Affiliations

Department of Animal Health and Management, Alagappa University, Karaikudi- 630 003, India. manikandanramar@yahoo.co.inNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21925163

Citation

Manikandan, Ramar, et al. "Ameliorative Effects of Curcumin Against Renal Injuries Mediated By Inducible Nitric Oxide Synthase and Nuclear Factor Kappa B During Gentamicin-induced Toxicity in Wistar Rats." European Journal of Pharmacology, vol. 670, no. 2-3, 2011, pp. 578-85.
Manikandan R, Beulaja M, Thiagarajan R, et al. Ameliorative effects of curcumin against renal injuries mediated by inducible nitric oxide synthase and nuclear factor kappa B during gentamicin-induced toxicity in Wistar rats. Eur J Pharmacol. 2011;670(2-3):578-85.
Manikandan, R., Beulaja, M., Thiagarajan, R., Priyadarsini, A., Saravanan, R., & Arumugam, M. (2011). Ameliorative effects of curcumin against renal injuries mediated by inducible nitric oxide synthase and nuclear factor kappa B during gentamicin-induced toxicity in Wistar rats. European Journal of Pharmacology, 670(2-3), 578-85. https://doi.org/10.1016/j.ejphar.2011.08.037
Manikandan R, et al. Ameliorative Effects of Curcumin Against Renal Injuries Mediated By Inducible Nitric Oxide Synthase and Nuclear Factor Kappa B During Gentamicin-induced Toxicity in Wistar Rats. Eur J Pharmacol. 2011 Nov 30;670(2-3):578-85. PubMed PMID: 21925163.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ameliorative effects of curcumin against renal injuries mediated by inducible nitric oxide synthase and nuclear factor kappa B during gentamicin-induced toxicity in Wistar rats. AU - Manikandan,Ramar, AU - Beulaja,Manikandan, AU - Thiagarajan,Raman, AU - Priyadarsini,Asokan, AU - Saravanan,Rajendran, AU - Arumugam,Munusamy, Y1 - 2011/09/10/ PY - 2011/03/08/received PY - 2011/08/15/revised PY - 2011/08/27/accepted PY - 2011/9/20/entrez PY - 2011/9/20/pubmed PY - 2012/3/1/medline SP - 578 EP - 85 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 670 IS - 2-3 N2 - The ameliorative role of curcumin in attenuating gentamicin-induced nephrotoxicity has been reported earlier however, the mechanism of action remains unclear. Gentamicin was injected intraperitoneally (100 mg/kg body weight) once daily for 6 days. Curcumin was administered orally (200 mg/kg body weight) once daily for 7, 15 and 30 days. Gentamicin-induced rats showed significant increase in the levels of kidney markers and the activities of urinary marker enzymes, which was reversed upon curcumin treatment. A significant increase in kidney lipid peroxidation (LPO) and decrease in activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-S-transferase (GST) and reduced glutathione (GSH) were observed in gentamicin-induced rats. Immunohistochemical, Western blot and RT-PCR studies in gentamicin-induced rats also demonstrated an increase in the levels of inducible nitric oxide synthase (iNOS) and nuclear factor-κB (NF-κB). All these effects induced by gentamicin were reduced upon treatment with curcumin in a time dependent manner. To conclude, curcumin enhances antioxidants, and decreases iNOS and NF-κB, thereby protecting the cells against oxidative stress induced by gentamicin. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/21925163/Ameliorative_effects_of_curcumin_against_renal_injuries_mediated_by_inducible_nitric_oxide_synthase_and_nuclear_factor_kappa_B_during_gentamicin_induced_toxicity_in_Wistar_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(11)00947-2 DB - PRIME DP - Unbound Medicine ER -