TY - JOUR T1 - Combined microwave-assisted extraction and high-speed counter-current chromatography for separation and purification of xanthones from Garcinia mangostana. AU - Fang,Lei, AU - Liu,Yuqin, AU - Zhuang,Huiyong, AU - Liu,Wei, AU - Wang,Xiao, AU - Huang,Luqi, Y1 - 2011/09/02/ PY - 2011/06/15/received PY - 2011/08/25/revised PY - 2011/08/28/accepted PY - 2011/9/20/entrez PY - 2011/9/20/pubmed PY - 2011/12/13/medline SP - 3023 EP - 7 JF - Journal of chromatography. B, Analytical technologies in the biomedical and life sciences JO - J Chromatogr B Analyt Technol Biomed Life Sci VL - 879 IS - 28 N2 - A microwave-assisted extraction (MAE) method is presented for the extraction of xanthones, α-mangostin and γ-mangostin from Garcinia mangostana. The MAE conditions including extraction temperature, liquid/solid ratio, extraction time and concentration of ethanol were optimized with an orthogonal test, and 5 g sample was extracted with the optimized conditions. The crude extraction of MAE was successfully isolated and purified by high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of petroleum ether-ethyl acetate-methanol-water (0.8:0.8:1:0.6, v/v) in one-step separation. The separation yielded 75 mg of α-mangostin at 98.5% purity, and 16 mg of γ-mangostin at 98.1% purity from 360 mg crude extract of G. mangostana in less than 7h. The purity of the two xanthones was determined by HPLC. Their structures were further identified by ESI-MS, (1)H NMR and (13)C NMR. SN - 1873-376X UR - https://www.unboundmedicine.com/medline/citation/21925972/Combined_microwave_assisted_extraction_and_high_speed_counter_current_chromatography_for_separation_and_purification_of_xanthones_from_Garcinia_mangostana_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1570-0232(11)00577-0 DB - PRIME DP - Unbound Medicine ER -