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Overexpression of a cytochrome P450 monooxygenase, CYP6ER1, is associated with resistance to imidacloprid in the brown planthopper, Nilaparvata lugens.
Insect Mol Biol. 2011 Dec; 20(6):763-73.IM

Abstract

The brown planthopper, Nilaparvata lugens, is an economically significant pest of rice throughout Asia and has evolved resistance to many insecticides including the neonicotinoid imidacloprid. The resistance of field populations of N. lugens to imidacloprid has been attributed to enhanced detoxification by cytochrome P450 monooxygenases (P450s), although, to date, the causative P450(s) has (have) not been identified. In the present study, biochemical assays using the model substrate 7-ethoxycoumarin showed enhanced P450 activity in several resistant N. lugens field strains when compared with a susceptible reference strain. Thirty three cDNA sequences encoding tentative unique P450s were identified from two recent sequencing projects and by degenerate PCR. The mRNA expression level of 32 of these was examined in susceptible, moderately resistant and highly resistant N. lugens strains using quantitative real-time PCR. A single P450 gene (CYP6ER1) was highly overexpressed in all resistant strains (up to 40-fold) and the level of expression observed in the different N. lugens strains was significantly correlated with the resistance phenotype. These results provide strong evidence for a role of CYP6ER1 in the resistance of N. lugens to imidacloprid.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Bass C
Centre for Sustainable Pest and Disease Management, Rothamsted Research, Harpenden, UK. chris.bass@bbsrc.ac.uk
Carvalho RA
No affiliation info available
Oliphant L
No affiliation info available
Puinean AM
No affiliation info available
Field LM
No affiliation info available
Nauen R
No affiliation info available
Williamson MS
No affiliation info available
Moores G
No affiliation info available
Gorman K
No affiliation info available

MeSH

Amino Acid SequenceAnimalsCytochrome P-450 Enzyme SystemDNA, ComplementaryFemaleGene DosageHemipteraImidazolesInsect ProteinsInsecticide ResistanceInsecticidesMolecular Sequence DataNeonicotinoidsNitro CompoundsPolymerase Chain Reaction

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21929695

Citation

Bass, C, et al. "Overexpression of a Cytochrome P450 Monooxygenase, CYP6ER1, Is Associated With Resistance to Imidacloprid in the Brown Planthopper, Nilaparvata Lugens." Insect Molecular Biology, vol. 20, no. 6, 2011, pp. 763-73.
Bass C, Carvalho RA, Oliphant L, et al. Overexpression of a cytochrome P450 monooxygenase, CYP6ER1, is associated with resistance to imidacloprid in the brown planthopper, Nilaparvata lugens. Insect Mol Biol. 2011;20(6):763-73.
Bass, C., Carvalho, R. A., Oliphant, L., Puinean, A. M., Field, L. M., Nauen, R., Williamson, M. S., Moores, G., & Gorman, K. (2011). Overexpression of a cytochrome P450 monooxygenase, CYP6ER1, is associated with resistance to imidacloprid in the brown planthopper, Nilaparvata lugens. Insect Molecular Biology, 20(6), 763-73. https://doi.org/10.1111/j.1365-2583.2011.01105.x
Bass C, et al. Overexpression of a Cytochrome P450 Monooxygenase, CYP6ER1, Is Associated With Resistance to Imidacloprid in the Brown Planthopper, Nilaparvata Lugens. Insect Mol Biol. 2011;20(6):763-73. PubMed PMID: 21929695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Overexpression of a cytochrome P450 monooxygenase, CYP6ER1, is associated with resistance to imidacloprid in the brown planthopper, Nilaparvata lugens. AU - Bass,C, AU - Carvalho,R A, AU - Oliphant,L, AU - Puinean,A M, AU - Field,L M, AU - Nauen,R, AU - Williamson,M S, AU - Moores,G, AU - Gorman,K, Y1 - 2011/09/19/ PY - 2011/9/21/entrez PY - 2011/9/21/pubmed PY - 2012/2/11/medline SP - 763 EP - 73 JF - Insect molecular biology JO - Insect Mol Biol VL - 20 IS - 6 N2 - The brown planthopper, Nilaparvata lugens, is an economically significant pest of rice throughout Asia and has evolved resistance to many insecticides including the neonicotinoid imidacloprid. The resistance of field populations of N. lugens to imidacloprid has been attributed to enhanced detoxification by cytochrome P450 monooxygenases (P450s), although, to date, the causative P450(s) has (have) not been identified. In the present study, biochemical assays using the model substrate 7-ethoxycoumarin showed enhanced P450 activity in several resistant N. lugens field strains when compared with a susceptible reference strain. Thirty three cDNA sequences encoding tentative unique P450s were identified from two recent sequencing projects and by degenerate PCR. The mRNA expression level of 32 of these was examined in susceptible, moderately resistant and highly resistant N. lugens strains using quantitative real-time PCR. A single P450 gene (CYP6ER1) was highly overexpressed in all resistant strains (up to 40-fold) and the level of expression observed in the different N. lugens strains was significantly correlated with the resistance phenotype. These results provide strong evidence for a role of CYP6ER1 in the resistance of N. lugens to imidacloprid. SN - 1365-2583 UR - https://www.unboundmedicine.com/medline/citation/21929695/Overexpression_of_a_cytochrome_P450_monooxygenase_CYP6ER1_is_associated_with_resistance_to_imidacloprid_in_the_brown_planthopper_Nilaparvata_lugens_ L2 - https://doi.org/10.1111/j.1365-2583.2011.01105.x DB - PRIME DP - Unbound Medicine ER -