Tags

Type your tag names separated by a space and hit enter

Multiple factors in the early splicing complex are involved in the nuclear retention of pre-mRNAs in mammalian cells.
Genes Cells. 2011 Oct; 16(10):1035-49.GC

Abstract

Intron-containing pre-mRNAs are retained in the nucleus until they are spliced. This mechanism is essential for proper gene expression. Although the formation of splicing complexes on pre-mRNAs is thought to be responsible for this nuclear retention activity, the details are poorly understood. In mammalian cells, in particular, very little information is available regarding the retention factors. Using a model reporter gene, we show here that U1 snRNP and U2AF but not U2 snRNP are essential for the nuclear retention of pre-mRNAs in mammalian cells, showing that E complex is the major entity responsible for the nuclear retention of pre-mRNAs in mammalian cells. By focusing on factors that bind to the 3'-splice site region, we found that the 65-kD subunit of U2AF (U2AF(65)) is important for nuclear retention and that its multiple domains have nuclear retention activity per se. We also provide evidence that UAP56, a DExD-box RNA helicase involved in both RNA splicing and export, cooperates with U2AF(65) in exerting nuclear retention activity. Our findings provide new information regarding the pre-mRNA nuclear retention factors in mammalian cells.

Authors+Show Affiliations

Institute for Virus Research, Kyoto University, Shogoin Kawahara-cho, Sakyo-ku, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21929696

Citation

Takemura, Reiko, et al. "Multiple Factors in the Early Splicing Complex Are Involved in the Nuclear Retention of pre-mRNAs in Mammalian Cells." Genes to Cells : Devoted to Molecular & Cellular Mechanisms, vol. 16, no. 10, 2011, pp. 1035-49.
Takemura R, Takeiwa T, Taniguchi I, et al. Multiple factors in the early splicing complex are involved in the nuclear retention of pre-mRNAs in mammalian cells. Genes Cells. 2011;16(10):1035-49.
Takemura, R., Takeiwa, T., Taniguchi, I., McCloskey, A., & Ohno, M. (2011). Multiple factors in the early splicing complex are involved in the nuclear retention of pre-mRNAs in mammalian cells. Genes to Cells : Devoted to Molecular & Cellular Mechanisms, 16(10), 1035-49. https://doi.org/10.1111/j.1365-2443.2011.01548.x
Takemura R, et al. Multiple Factors in the Early Splicing Complex Are Involved in the Nuclear Retention of pre-mRNAs in Mammalian Cells. Genes Cells. 2011;16(10):1035-49. PubMed PMID: 21929696.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiple factors in the early splicing complex are involved in the nuclear retention of pre-mRNAs in mammalian cells. AU - Takemura,Reiko, AU - Takeiwa,Toshihiko, AU - Taniguchi,Ichiro, AU - McCloskey,Asako, AU - Ohno,Mutsuhito, Y1 - 2011/09/19/ PY - 2011/9/21/entrez PY - 2011/9/21/pubmed PY - 2012/1/14/medline SP - 1035 EP - 49 JF - Genes to cells : devoted to molecular & cellular mechanisms JO - Genes Cells VL - 16 IS - 10 N2 - Intron-containing pre-mRNAs are retained in the nucleus until they are spliced. This mechanism is essential for proper gene expression. Although the formation of splicing complexes on pre-mRNAs is thought to be responsible for this nuclear retention activity, the details are poorly understood. In mammalian cells, in particular, very little information is available regarding the retention factors. Using a model reporter gene, we show here that U1 snRNP and U2AF but not U2 snRNP are essential for the nuclear retention of pre-mRNAs in mammalian cells, showing that E complex is the major entity responsible for the nuclear retention of pre-mRNAs in mammalian cells. By focusing on factors that bind to the 3'-splice site region, we found that the 65-kD subunit of U2AF (U2AF(65)) is important for nuclear retention and that its multiple domains have nuclear retention activity per se. We also provide evidence that UAP56, a DExD-box RNA helicase involved in both RNA splicing and export, cooperates with U2AF(65) in exerting nuclear retention activity. Our findings provide new information regarding the pre-mRNA nuclear retention factors in mammalian cells. SN - 1365-2443 UR - https://www.unboundmedicine.com/medline/citation/21929696/Multiple_factors_in_the_early_splicing_complex_are_involved_in_the_nuclear_retention_of_pre_mRNAs_in_mammalian_cells_ L2 - https://doi.org/10.1111/j.1365-2443.2011.01548.x DB - PRIME DP - Unbound Medicine ER -