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Superior thermotolerance of Saccharomyces cerevisiae for efficient bioethanol fermentation can be achieved by overexpression of RSP5 ubiquitin ligase.
Biotechnol Adv. 2012 Nov-Dec; 30(6):1289-300.BA

Abstract

The simultaneous saccharification and fermentation process requires thermo-tolerant yeast to facilitate the enzymatic hydrolysis of cellulose. In this paper, we describe a Htg+ strain that exhibits confluent growth at high temperature (41 °C) and resistance to heat shock, ethanol, osmotic, oxidative and DNA damage stresses. HTG6, one of the six genes responsible for the thermotolerant phenotype was identified to be the gene RSP5 encoding a ubiquitin ligase. The RSP5 allele of the Htg+ strain, designated RSP5-C, possessed five, one and two base changes in the promoter, open reading frame and terminator region, respectively. The base changes in the promoter region of the RSP5-C allele were found to be responsible for the thermotolerant phenotype by strongly increasing transcription of the RSP5 gene and consequently causing a rise in the ubiquitination of cell proteins. Overexpression of the RSP5-BY allele present in the htg6 host strain (Htg-) conferred thermotolerance at 41°C, to this strain as in the case of RSP5-C allele. We also discovered that an Htg+ strain overexpressing the RSP5-C allele exhibits a more robust Htg+ phenotype against higher temperature (43 °C). The data presented here also suggest that overexpression of RSP5 could be applied to raise the upper limit of thermotolerance in S. cerevisiae strain used for industrial bioethanol production.

Authors+Show Affiliations

Department of Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21930195

Citation

Shahsavarani, Hosein, et al. "Superior Thermotolerance of Saccharomyces Cerevisiae for Efficient Bioethanol Fermentation Can Be Achieved By Overexpression of RSP5 Ubiquitin Ligase." Biotechnology Advances, vol. 30, no. 6, 2012, pp. 1289-300.
Shahsavarani H, Sugiyama M, Kaneko Y, et al. Superior thermotolerance of Saccharomyces cerevisiae for efficient bioethanol fermentation can be achieved by overexpression of RSP5 ubiquitin ligase. Biotechnol Adv. 2012;30(6):1289-300.
Shahsavarani, H., Sugiyama, M., Kaneko, Y., Chuenchit, B., & Harashima, S. (2012). Superior thermotolerance of Saccharomyces cerevisiae for efficient bioethanol fermentation can be achieved by overexpression of RSP5 ubiquitin ligase. Biotechnology Advances, 30(6), 1289-300. https://doi.org/10.1016/j.biotechadv.2011.09.002
Shahsavarani H, et al. Superior Thermotolerance of Saccharomyces Cerevisiae for Efficient Bioethanol Fermentation Can Be Achieved By Overexpression of RSP5 Ubiquitin Ligase. Biotechnol Adv. 2012 Nov-Dec;30(6):1289-300. PubMed PMID: 21930195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Superior thermotolerance of Saccharomyces cerevisiae for efficient bioethanol fermentation can be achieved by overexpression of RSP5 ubiquitin ligase. AU - Shahsavarani,Hosein, AU - Sugiyama,Minetaka, AU - Kaneko,Yoshinobu, AU - Chuenchit,Boonchird, AU - Harashima,Satoshi, Y1 - 2011/09/10/ PY - 2011/07/15/received PY - 2011/08/20/revised PY - 2011/09/04/accepted PY - 2011/9/21/entrez PY - 2011/9/21/pubmed PY - 2013/4/6/medline SP - 1289 EP - 300 JF - Biotechnology advances JO - Biotechnol Adv VL - 30 IS - 6 N2 - The simultaneous saccharification and fermentation process requires thermo-tolerant yeast to facilitate the enzymatic hydrolysis of cellulose. In this paper, we describe a Htg+ strain that exhibits confluent growth at high temperature (41 °C) and resistance to heat shock, ethanol, osmotic, oxidative and DNA damage stresses. HTG6, one of the six genes responsible for the thermotolerant phenotype was identified to be the gene RSP5 encoding a ubiquitin ligase. The RSP5 allele of the Htg+ strain, designated RSP5-C, possessed five, one and two base changes in the promoter, open reading frame and terminator region, respectively. The base changes in the promoter region of the RSP5-C allele were found to be responsible for the thermotolerant phenotype by strongly increasing transcription of the RSP5 gene and consequently causing a rise in the ubiquitination of cell proteins. Overexpression of the RSP5-BY allele present in the htg6 host strain (Htg-) conferred thermotolerance at 41°C, to this strain as in the case of RSP5-C allele. We also discovered that an Htg+ strain overexpressing the RSP5-C allele exhibits a more robust Htg+ phenotype against higher temperature (43 °C). The data presented here also suggest that overexpression of RSP5 could be applied to raise the upper limit of thermotolerance in S. cerevisiae strain used for industrial bioethanol production. SN - 1873-1899 UR - https://www.unboundmedicine.com/medline/citation/21930195/Superior_thermotolerance_of_Saccharomyces_cerevisiae_for_efficient_bioethanol_fermentation_can_be_achieved_by_overexpression_of_RSP5_ubiquitin_ligase_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0734-9750(11)00155-8 DB - PRIME DP - Unbound Medicine ER -