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Human papillomavirus type 16 oropharyngeal cancers in lymph nodes as a marker of metastases.
Arch Otolaryngol Head Neck Surg. 2011 Sep; 137(9):910-4.AO

Abstract

BACKGROUND

Oropharyngeal squamous cell carcinomas (OSCCs) are associated with high-grade human papillomavirus (HPV) infection in 20% to 30% of cases. HPV-16 DNA has been detected in cervical lymph node metastases of HPV-16(+) OSCC. However, the meaning of HPV-16 DNA detection in lymph nodes remains controversial. Does the presence of HPV-16 DNA in lymph nodes correlate with their metastatic involvement, or is it just a consequence of the filter function of lymph nodes?

METHODS

Viral load quantification using reverse transcriptase-polymerase chain reaction was retrospectively performed in primary tumors and in cervical lymph nodes, originating from levels IIa, IIb, and III, in 11 patients with HPV-16(+) OSCC and in 3 control patients with HPV-16(-) OSCC.

RESULTS

A total of 45 lymph node levels were analyzed. HPV-16 DNA was not detected in HPV-16(-) OSCC lymph nodes. No statistically significant difference was found between primary tumors and metastatic lymph nodes viral load (P > .01). The viral load value was significantly higher in metastatic lymph nodes than in tumor-free lymph nodes (P < .01). Among 27 tumor-free lymph node levels, the viral load value was undetectable in 16, low or medium (<10(5) copies per million cells) in 8, and high (>10(5) copies per million cells) in 3.

CONCLUSIONS

HPV-16 DNA detection in lymph nodes of patients affected with HPV-16(+) oropharyngeal cancer is indicative of metastatic involvement. Tumor-free lymph nodes with a high viral load value would suggest the presence of occult lymph nodes metastasis and the opportunity to use HPV-16 DNA as a metastatic marker. Further investigations are needed.

Authors+Show Affiliations

Department of Otolaryngology-Head and Neck Surgery, Faculty of Medicine, University Pierre et Marie Curie Paris VI, France. mirghani_fr@yahoo.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21930979

Citation

Mirghani, Haïtham, et al. "Human Papillomavirus Type 16 Oropharyngeal Cancers in Lymph Nodes as a Marker of Metastases." Archives of Otolaryngology--head & Neck Surgery, vol. 137, no. 9, 2011, pp. 910-4.
Mirghani H, Moreau F, Lefèvre M, et al. Human papillomavirus type 16 oropharyngeal cancers in lymph nodes as a marker of metastases. Arch Otolaryngol Head Neck Surg. 2011;137(9):910-4.
Mirghani, H., Moreau, F., Lefèvre, M., Tam, C., Périé, S., Soussan, P., & St Guily, J. L. (2011). Human papillomavirus type 16 oropharyngeal cancers in lymph nodes as a marker of metastases. Archives of Otolaryngology--head & Neck Surgery, 137(9), 910-4. https://doi.org/10.1001/archoto.2011.141
Mirghani H, et al. Human Papillomavirus Type 16 Oropharyngeal Cancers in Lymph Nodes as a Marker of Metastases. Arch Otolaryngol Head Neck Surg. 2011;137(9):910-4. PubMed PMID: 21930979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human papillomavirus type 16 oropharyngeal cancers in lymph nodes as a marker of metastases. AU - Mirghani,Haïtham, AU - Moreau,Frederique, AU - Lefèvre,Marine, AU - Tam,Chong, AU - Périé,Sophie, AU - Soussan,Patrick, AU - St Guily,Jean Lacau, PY - 2011/9/21/entrez PY - 2011/9/21/pubmed PY - 2011/11/11/medline SP - 910 EP - 4 JF - Archives of otolaryngology--head & neck surgery JO - Arch. Otolaryngol. Head Neck Surg. VL - 137 IS - 9 N2 - BACKGROUND: Oropharyngeal squamous cell carcinomas (OSCCs) are associated with high-grade human papillomavirus (HPV) infection in 20% to 30% of cases. HPV-16 DNA has been detected in cervical lymph node metastases of HPV-16(+) OSCC. However, the meaning of HPV-16 DNA detection in lymph nodes remains controversial. Does the presence of HPV-16 DNA in lymph nodes correlate with their metastatic involvement, or is it just a consequence of the filter function of lymph nodes? METHODS: Viral load quantification using reverse transcriptase-polymerase chain reaction was retrospectively performed in primary tumors and in cervical lymph nodes, originating from levels IIa, IIb, and III, in 11 patients with HPV-16(+) OSCC and in 3 control patients with HPV-16(-) OSCC. RESULTS: A total of 45 lymph node levels were analyzed. HPV-16 DNA was not detected in HPV-16(-) OSCC lymph nodes. No statistically significant difference was found between primary tumors and metastatic lymph nodes viral load (P > .01). The viral load value was significantly higher in metastatic lymph nodes than in tumor-free lymph nodes (P < .01). Among 27 tumor-free lymph node levels, the viral load value was undetectable in 16, low or medium (<10(5) copies per million cells) in 8, and high (>10(5) copies per million cells) in 3. CONCLUSIONS: HPV-16 DNA detection in lymph nodes of patients affected with HPV-16(+) oropharyngeal cancer is indicative of metastatic involvement. Tumor-free lymph nodes with a high viral load value would suggest the presence of occult lymph nodes metastasis and the opportunity to use HPV-16 DNA as a metastatic marker. Further investigations are needed. SN - 1538-361X UR - https://www.unboundmedicine.com/medline/citation/21930979/Human_papillomavirus_type_16_oropharyngeal_cancers_in_lymph_nodes_as_a_marker_of_metastases_ L2 - https://jamanetwork.com/journals/jamaotolaryngology/fullarticle/10.1001/archoto.2011.141 DB - PRIME DP - Unbound Medicine ER -