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Phenotypic characterization and prevalence of enterotoxin genes in Staphylococcus aureus isolates from outbreaks of illness in Chengdu City.
Foodborne Pathog Dis. 2011 Dec; 8(12):1317-20.FP

Abstract

Staphylococcus aureus produces a spectrum of enterotoxin that is recognized as the main reason for causing staphylococcal food poisoning. The aim of the current study was to investigate the phenotypic characteristics and enterotoxin genotypes of S. aureus isolated from food poisoning sufferers. On the basis of the amplification of 16S rRNA and nuc gene specific to S. aureus assay and the phenotype (hemolytic activity, thermal stable nuclease [Tnase] test, and biofilm formation), all isolates were identified as S. aureus. To genotypically characterize S. aureus isolates, genes encoding staphylococcal enterotoxin (sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sem, sen, ser, and seu) were investigated by using polymerase chain reaction technique. The results showed that the eight isolates of S. aureus had different enterotoxin genotypic characteristics, which was the main cause of food poisoning. One isolate contained 10 enterotoxin genes, and the other 7 isolates carried 3 or more enterotoxin genes. The frequency of the newly identified enterotoxin genes (seg-seu) was higher than classical genes (sea-see). Overall, multi-gene detection rates were 75% (for sek, ser, and seu); 50% (for sea and sem); 37.5% (for sen, seg, and sei); and 12.5% (for seb, sec, sed, and sej), respectively. The see and seh gene were not detected in any isolates. The current study provided the exact distribution of enterotoxin genes in eight S. aureus strains from food poisoning sufferers, which indicated that the pathogenicity of the newly identified enterotoxin should be highlighted. The need for prevention of food poisoning occurrences caused by enterotoxin of S. aureus should be reinforced.

Authors+Show Affiliations

College of Life Science and Technology, Southwest University for Nationalities, Chengdu, Sichuan, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21939349

Citation

Tang, Junni, et al. "Phenotypic Characterization and Prevalence of Enterotoxin Genes in Staphylococcus Aureus Isolates From Outbreaks of Illness in Chengdu City." Foodborne Pathogens and Disease, vol. 8, no. 12, 2011, pp. 1317-20.
Tang J, Tang C, Chen J, et al. Phenotypic characterization and prevalence of enterotoxin genes in Staphylococcus aureus isolates from outbreaks of illness in Chengdu City. Foodborne Pathog Dis. 2011;8(12):1317-20.
Tang, J., Tang, C., Chen, J., Du, Y., Yang, X. N., Wang, C., Zhang, H., & Yue, H. (2011). Phenotypic characterization and prevalence of enterotoxin genes in Staphylococcus aureus isolates from outbreaks of illness in Chengdu City. Foodborne Pathogens and Disease, 8(12), 1317-20. https://doi.org/10.1089/fpd.2011.0924
Tang J, et al. Phenotypic Characterization and Prevalence of Enterotoxin Genes in Staphylococcus Aureus Isolates From Outbreaks of Illness in Chengdu City. Foodborne Pathog Dis. 2011;8(12):1317-20. PubMed PMID: 21939349.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phenotypic characterization and prevalence of enterotoxin genes in Staphylococcus aureus isolates from outbreaks of illness in Chengdu City. AU - Tang,Junni, AU - Tang,Cheng, AU - Chen,Juan, AU - Du,Yiwu, AU - Yang,Xiao-nong, AU - Wang,Changting, AU - Zhang,Huanrong, AU - Yue,Hua, Y1 - 2011/09/22/ PY - 2011/9/24/entrez PY - 2011/9/24/pubmed PY - 2013/6/20/medline SP - 1317 EP - 20 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 8 IS - 12 N2 - Staphylococcus aureus produces a spectrum of enterotoxin that is recognized as the main reason for causing staphylococcal food poisoning. The aim of the current study was to investigate the phenotypic characteristics and enterotoxin genotypes of S. aureus isolated from food poisoning sufferers. On the basis of the amplification of 16S rRNA and nuc gene specific to S. aureus assay and the phenotype (hemolytic activity, thermal stable nuclease [Tnase] test, and biofilm formation), all isolates were identified as S. aureus. To genotypically characterize S. aureus isolates, genes encoding staphylococcal enterotoxin (sea, seb, sec, sed, see, seg, seh, sei, sej, sek, sem, sen, ser, and seu) were investigated by using polymerase chain reaction technique. The results showed that the eight isolates of S. aureus had different enterotoxin genotypic characteristics, which was the main cause of food poisoning. One isolate contained 10 enterotoxin genes, and the other 7 isolates carried 3 or more enterotoxin genes. The frequency of the newly identified enterotoxin genes (seg-seu) was higher than classical genes (sea-see). Overall, multi-gene detection rates were 75% (for sek, ser, and seu); 50% (for sea and sem); 37.5% (for sen, seg, and sei); and 12.5% (for seb, sec, sed, and sej), respectively. The see and seh gene were not detected in any isolates. The current study provided the exact distribution of enterotoxin genes in eight S. aureus strains from food poisoning sufferers, which indicated that the pathogenicity of the newly identified enterotoxin should be highlighted. The need for prevention of food poisoning occurrences caused by enterotoxin of S. aureus should be reinforced. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/21939349/Phenotypic_characterization_and_prevalence_of_enterotoxin_genes_in_Staphylococcus_aureus_isolates_from_outbreaks_of_illness_in_Chengdu_City_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2011.0924?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -