Tags

Type your tag names separated by a space and hit enter

A multi-amplification aptasensor for highly sensitive detection of thrombin based on high-quality hollow CoPt nanoparticles decorated graphene.
Biosens Bioelectron. 2011 Dec 15; 30(1):61-6.BB

Abstract

In this work, we have successfully demonstrated a facile strategy to incorporate high-quality hollow CoPt bimetal alloy nanoparticles (HCoPt) onto reduced graphene oxide sheet (HCoPt-RGs). An advanced sandwich-type electrochemical aptasensor for thrombin was proposed by using the HCoPt-RGs conjugates as secondary label. The formed conjugates provided large surface area for loading plentiful redox probe thionine (Thi), horseradish peroxidase (HRP) and secondary aptamer (Apt II) with good stability and friendly biocompatibility, indicating their superior properties in electroactive mediator enrichment and biomolecule immobilization. Furthermore, activated by glutaraldehyde (GA), the chitosan-hollow CoPt alloy nanoparticle (CS-HCoPt) film can greatly facilitate the capture of primary aptamer (Apt I) and dramatically reduce the nonspecific binding. Excellent sensitivity was obtained by detecting the conspicuously enhanced electrochemical signal of Thi, which was amplified by HCoPt alloy nanoparticles and HRP toward the catalytic reduction of H(2)O(2). The aptasensor displayed excellent performance for thrombin with a wide linearity in the range from 1.0×10(-12) to 5.0×10(-8) M and a relatively low detection limit of 3.4×10(-13) M. Moreover, the resulted aptasensor also exhibited good specificity, acceptable reproducibility and stability, indicating that the present strategy could pave a promising way for the wide application of graphene in clinical research.

Authors+Show Affiliations

Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21944185

Citation

Wang, Yan, et al. "A Multi-amplification Aptasensor for Highly Sensitive Detection of Thrombin Based On High-quality Hollow CoPt Nanoparticles Decorated Graphene." Biosensors & Bioelectronics, vol. 30, no. 1, 2011, pp. 61-6.
Wang Y, Yuan R, Chai Y, et al. A multi-amplification aptasensor for highly sensitive detection of thrombin based on high-quality hollow CoPt nanoparticles decorated graphene. Biosens Bioelectron. 2011;30(1):61-6.
Wang, Y., Yuan, R., Chai, Y., Yuan, Y., Bai, L., & Liao, Y. (2011). A multi-amplification aptasensor for highly sensitive detection of thrombin based on high-quality hollow CoPt nanoparticles decorated graphene. Biosensors & Bioelectronics, 30(1), 61-6. https://doi.org/10.1016/j.bios.2011.08.027
Wang Y, et al. A Multi-amplification Aptasensor for Highly Sensitive Detection of Thrombin Based On High-quality Hollow CoPt Nanoparticles Decorated Graphene. Biosens Bioelectron. 2011 Dec 15;30(1):61-6. PubMed PMID: 21944185.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A multi-amplification aptasensor for highly sensitive detection of thrombin based on high-quality hollow CoPt nanoparticles decorated graphene. AU - Wang,Yan, AU - Yuan,Ruo, AU - Chai,Yaqin, AU - Yuan,Yali, AU - Bai,Lijuan, AU - Liao,Yuhong, Y1 - 2011/08/27/ PY - 2011/06/10/received PY - 2011/08/19/revised PY - 2011/08/22/accepted PY - 2011/9/28/entrez PY - 2011/9/29/pubmed PY - 2012/3/20/medline SP - 61 EP - 6 JF - Biosensors & bioelectronics JO - Biosens Bioelectron VL - 30 IS - 1 N2 - In this work, we have successfully demonstrated a facile strategy to incorporate high-quality hollow CoPt bimetal alloy nanoparticles (HCoPt) onto reduced graphene oxide sheet (HCoPt-RGs). An advanced sandwich-type electrochemical aptasensor for thrombin was proposed by using the HCoPt-RGs conjugates as secondary label. The formed conjugates provided large surface area for loading plentiful redox probe thionine (Thi), horseradish peroxidase (HRP) and secondary aptamer (Apt II) with good stability and friendly biocompatibility, indicating their superior properties in electroactive mediator enrichment and biomolecule immobilization. Furthermore, activated by glutaraldehyde (GA), the chitosan-hollow CoPt alloy nanoparticle (CS-HCoPt) film can greatly facilitate the capture of primary aptamer (Apt I) and dramatically reduce the nonspecific binding. Excellent sensitivity was obtained by detecting the conspicuously enhanced electrochemical signal of Thi, which was amplified by HCoPt alloy nanoparticles and HRP toward the catalytic reduction of H(2)O(2). The aptasensor displayed excellent performance for thrombin with a wide linearity in the range from 1.0×10(-12) to 5.0×10(-8) M and a relatively low detection limit of 3.4×10(-13) M. Moreover, the resulted aptasensor also exhibited good specificity, acceptable reproducibility and stability, indicating that the present strategy could pave a promising way for the wide application of graphene in clinical research. SN - 1873-4235 UR - https://www.unboundmedicine.com/medline/citation/21944185/A_multi_amplification_aptasensor_for_highly_sensitive_detection_of_thrombin_based_on_high_quality_hollow_CoPt_nanoparticles_decorated_graphene_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0956-5663(11)00584-7 DB - PRIME DP - Unbound Medicine ER -