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A sample preparation method for gold nanoparticle-assisted laser desorption/ionization time-of-flight mass spectrometry.
Methods Mol Biol. 2011; 790:167-72.MM

Abstract

A sample preparation method to detect small molecules in laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS) was developed using bare gold nanoparticles (AuNPs) as matrices. In this sample preparation method, the analyte is deposited first and then followed by the bare AuNPs. Neutral steroids and carbohydrates, which are difficult to ionize, using organic matrices, are cationized efficiently by combining AuNP-assisted LDI-TOF MS with this sample preparation method. As compared to the dried-droplet method (i.e., analyte and bare AuNPs are mixed and dried together), this method offers distinct advantages for improving shot-to-shot reproducibility, increasing the ionization efficiency of the analyte, and reducing sample preparation time.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Lin YH
Department of Chemistry, National Sun Yat-sen University, Kaohsiung, Taiwan.
Tseng WL
No affiliation info available

MeSH

Analytic Sample Preparation MethodsGoldLasersMass SpectrometryMetal NanoparticlesSalts

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21948413

Citation

Lin, Yen-Hsiu, and Wei-Lung Tseng. "A Sample Preparation Method for Gold Nanoparticle-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry." Methods in Molecular Biology (Clifton, N.J.), vol. 790, 2011, pp. 167-72.
Lin YH, Tseng WL. A sample preparation method for gold nanoparticle-assisted laser desorption/ionization time-of-flight mass spectrometry. Methods Mol Biol. 2011;790:167-72.
Lin, Y. H., & Tseng, W. L. (2011). A sample preparation method for gold nanoparticle-assisted laser desorption/ionization time-of-flight mass spectrometry. Methods in Molecular Biology (Clifton, N.J.), 790, 167-72. https://doi.org/10.1007/978-1-61779-319-6_12
Lin YH, Tseng WL. A Sample Preparation Method for Gold Nanoparticle-assisted Laser Desorption/ionization Time-of-flight Mass Spectrometry. Methods Mol Biol. 2011;790:167-72. PubMed PMID: 21948413.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A sample preparation method for gold nanoparticle-assisted laser desorption/ionization time-of-flight mass spectrometry. AU - Lin,Yen-Hsiu, AU - Tseng,Wei-Lung, PY - 2011/9/28/entrez PY - 2011/9/29/pubmed PY - 2012/1/17/medline SP - 167 EP - 72 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 790 N2 - A sample preparation method to detect small molecules in laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS) was developed using bare gold nanoparticles (AuNPs) as matrices. In this sample preparation method, the analyte is deposited first and then followed by the bare AuNPs. Neutral steroids and carbohydrates, which are difficult to ionize, using organic matrices, are cationized efficiently by combining AuNP-assisted LDI-TOF MS with this sample preparation method. As compared to the dried-droplet method (i.e., analyte and bare AuNPs are mixed and dried together), this method offers distinct advantages for improving shot-to-shot reproducibility, increasing the ionization efficiency of the analyte, and reducing sample preparation time. SN - 1940-6029 UR - https://www.unboundmedicine.com/medline/citation/21948413/A_sample_preparation_method_for_gold_nanoparticle_assisted_laser_desorption/ionization_time_of_flight_mass_spectrometry_ L2 - https://dx.doi.org/10.1007/978-1-61779-319-6_12 DB - PRIME DP - Unbound Medicine ER -

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