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Validity of HMGB1 measurement in epithelial lining fluid in patients with COPD.
Eur J Clin Invest. 2012 Apr; 42(4):419-26.EJ

Abstract

BACKGROUND

It has been known that high-mobility group box 1 (HMGB1) plays an important role in the pathogenesis of various inflammatory disorders in the lung. We attempted to determine the validity of measurement of HMGB1 levels in epithelial lining fluid (ELF) from patients with chronic obstructive pulmonary disease (COPD).

MATERIALS AND METHODS

We measured HMGB1 levels in ELF separately obtained from central or peripheral airways using a bronchoscopic microsampling technique in 14 non-smokers, 13 smokers without COPD and 30 smokers with COPD. We also evaluated whether those levels were correlated with the indexes of pulmonary function and grade of low-attenuation area (LAA) on high-resolution computed tomographic scans.

RESULTS

HMGB1 levels in ELF from central airways did not significantly differ among the three groups. However, HMGB1 levels in peripheral airways were significantly higher in COPD patients than in non-smokers and smokers without COPD. Both the concentrations of interleukin-8 and human polymorphonuclear elastase in peripheral airways were also significantly higher in COPD patients. Moreover, those levels were significantly correlated with HMGB1 level. In addition, HMGB1 level in peripheral airways was closely correlated with the degree of airflow obstruction and grade of LAA in COPD patients.

CONCLUSIONS

HMGB1 levels in peripheral airways were elevated in smokers without COPD, as compared with non-smokers, and those levels were further augmented in COPD patients. Those levels were associated with the severity of COPD. Therefore, HMGB1 in peripheral airways may be a potentially interesting target for new pharmacological treatments in COPD patients.

Authors+Show Affiliations

Department of Respiratory Medicine, Graduate School of Medicine, Osaka City University, Osaka, Japan. kanazawa-h@med.osaka-cu.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Validation Study

Language

eng

PubMed ID

21950682

Citation

Kanazawa, Hiroshi, et al. "Validity of HMGB1 Measurement in Epithelial Lining Fluid in Patients With COPD." European Journal of Clinical Investigation, vol. 42, no. 4, 2012, pp. 419-26.
Kanazawa H, Tochino Y, Asai K, et al. Validity of HMGB1 measurement in epithelial lining fluid in patients with COPD. Eur J Clin Invest. 2012;42(4):419-26.
Kanazawa, H., Tochino, Y., Asai, K., Ichimaru, Y., Watanabe, T., & Hirata, K. (2012). Validity of HMGB1 measurement in epithelial lining fluid in patients with COPD. European Journal of Clinical Investigation, 42(4), 419-26. https://doi.org/10.1111/j.1365-2362.2011.02598.x
Kanazawa H, et al. Validity of HMGB1 Measurement in Epithelial Lining Fluid in Patients With COPD. Eur J Clin Invest. 2012;42(4):419-26. PubMed PMID: 21950682.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Validity of HMGB1 measurement in epithelial lining fluid in patients with COPD. AU - Kanazawa,Hiroshi, AU - Tochino,Yoshihiro, AU - Asai,Kazuhisa, AU - Ichimaru,Yukikazu, AU - Watanabe,Tetsuya, AU - Hirata,Kazuto, Y1 - 2011/09/23/ PY - 2011/9/29/entrez PY - 2011/9/29/pubmed PY - 2012/6/16/medline SP - 419 EP - 26 JF - European journal of clinical investigation JO - Eur J Clin Invest VL - 42 IS - 4 N2 - BACKGROUND: It has been known that high-mobility group box 1 (HMGB1) plays an important role in the pathogenesis of various inflammatory disorders in the lung. We attempted to determine the validity of measurement of HMGB1 levels in epithelial lining fluid (ELF) from patients with chronic obstructive pulmonary disease (COPD). MATERIALS AND METHODS: We measured HMGB1 levels in ELF separately obtained from central or peripheral airways using a bronchoscopic microsampling technique in 14 non-smokers, 13 smokers without COPD and 30 smokers with COPD. We also evaluated whether those levels were correlated with the indexes of pulmonary function and grade of low-attenuation area (LAA) on high-resolution computed tomographic scans. RESULTS: HMGB1 levels in ELF from central airways did not significantly differ among the three groups. However, HMGB1 levels in peripheral airways were significantly higher in COPD patients than in non-smokers and smokers without COPD. Both the concentrations of interleukin-8 and human polymorphonuclear elastase in peripheral airways were also significantly higher in COPD patients. Moreover, those levels were significantly correlated with HMGB1 level. In addition, HMGB1 level in peripheral airways was closely correlated with the degree of airflow obstruction and grade of LAA in COPD patients. CONCLUSIONS: HMGB1 levels in peripheral airways were elevated in smokers without COPD, as compared with non-smokers, and those levels were further augmented in COPD patients. Those levels were associated with the severity of COPD. Therefore, HMGB1 in peripheral airways may be a potentially interesting target for new pharmacological treatments in COPD patients. SN - 1365-2362 UR - https://www.unboundmedicine.com/medline/citation/21950682/Validity_of_HMGB1_measurement_in_epithelial_lining_fluid_in_patients_with_COPD_ L2 - https://doi.org/10.1111/j.1365-2362.2011.02598.x DB - PRIME DP - Unbound Medicine ER -