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Localization of seed oil body proteins in tobacco protoplasts reveals specific mechanisms of protein targeting to leaf lipid droplets.
J Integr Plant Biol. 2011 Nov; 53(11):858-68.JI

Abstract

Oleosin, caleosin and steroleosin are normally expressed in developing seed cells and are targeted to oil bodies. In the present work, the cDNA of each gene tagged with fluorescent proteins was transiently expressed into tobacco protoplasts and the fluorescent patterns observed by confocal laser scanning microscopy. Our results indicated clear differences in the endocellular localization of the three proteins. Oleosin and caleosin both share a common structure consisting of a central hydrophobic domain flanked by two hydrophilic domains and were correctly targeted to lipid droplets (LD), whereas steroleosin, characterized by an N-terminal oil body anchoring domain, was mainly retained in the endoplasmic reticulum (ER). Protoplast fractionation on sucrose gradients indicated that both oleosin and caleosin-green fluorescent protein (GFP) peaked at different fractions than where steroleosin-GFP or the ER marker binding immunoglobulin protein (BiP), were recovered. Chemical analysis confirmed the presence of triacylglycerols in one of the fractions where oleosin-GFP was recovered. Finally, only oleosin- and caleosin-GFP were able to reconstitute artificial oil bodies in the presence of triacylglycerols and phospholipids. Taken together, our results pointed out for the first time that leaf LDs can be separated by the ER and both oleosin or caleosin are selectively targeted due to the existence of selective mechanisms controlling protein association with these organelles.

Authors+Show Affiliations

Institute of Sciences of Food Production C.N.R. Unit of Lecce, via Monteroni, Lecce 73100, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21951961

Citation

De Domenico, Stefania, et al. "Localization of Seed Oil Body Proteins in Tobacco Protoplasts Reveals Specific Mechanisms of Protein Targeting to Leaf Lipid Droplets." Journal of Integrative Plant Biology, vol. 53, no. 11, 2011, pp. 858-68.
De Domenico S, Bonsegna S, Lenucci MS, et al. Localization of seed oil body proteins in tobacco protoplasts reveals specific mechanisms of protein targeting to leaf lipid droplets. J Integr Plant Biol. 2011;53(11):858-68.
De Domenico, S., Bonsegna, S., Lenucci, M. S., Poltronieri, P., Di Sansebastiano, G. P., & Santino, A. (2011). Localization of seed oil body proteins in tobacco protoplasts reveals specific mechanisms of protein targeting to leaf lipid droplets. Journal of Integrative Plant Biology, 53(11), 858-68. https://doi.org/10.1111/j.1744-7909.2011.01077.x
De Domenico S, et al. Localization of Seed Oil Body Proteins in Tobacco Protoplasts Reveals Specific Mechanisms of Protein Targeting to Leaf Lipid Droplets. J Integr Plant Biol. 2011;53(11):858-68. PubMed PMID: 21951961.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Localization of seed oil body proteins in tobacco protoplasts reveals specific mechanisms of protein targeting to leaf lipid droplets. AU - De Domenico,Stefania, AU - Bonsegna,Stefania, AU - Lenucci,Marcello Salvatore, AU - Poltronieri,Palmiro, AU - Di Sansebastiano,Gian Pietro, AU - Santino,Angelo, PY - 2011/9/29/entrez PY - 2011/9/29/pubmed PY - 2012/4/14/medline SP - 858 EP - 68 JF - Journal of integrative plant biology JO - J Integr Plant Biol VL - 53 IS - 11 N2 - Oleosin, caleosin and steroleosin are normally expressed in developing seed cells and are targeted to oil bodies. In the present work, the cDNA of each gene tagged with fluorescent proteins was transiently expressed into tobacco protoplasts and the fluorescent patterns observed by confocal laser scanning microscopy. Our results indicated clear differences in the endocellular localization of the three proteins. Oleosin and caleosin both share a common structure consisting of a central hydrophobic domain flanked by two hydrophilic domains and were correctly targeted to lipid droplets (LD), whereas steroleosin, characterized by an N-terminal oil body anchoring domain, was mainly retained in the endoplasmic reticulum (ER). Protoplast fractionation on sucrose gradients indicated that both oleosin and caleosin-green fluorescent protein (GFP) peaked at different fractions than where steroleosin-GFP or the ER marker binding immunoglobulin protein (BiP), were recovered. Chemical analysis confirmed the presence of triacylglycerols in one of the fractions where oleosin-GFP was recovered. Finally, only oleosin- and caleosin-GFP were able to reconstitute artificial oil bodies in the presence of triacylglycerols and phospholipids. Taken together, our results pointed out for the first time that leaf LDs can be separated by the ER and both oleosin or caleosin are selectively targeted due to the existence of selective mechanisms controlling protein association with these organelles. SN - 1744-7909 UR - https://www.unboundmedicine.com/medline/citation/21951961/Localization_of_seed_oil_body_proteins_in_tobacco_protoplasts_reveals_specific_mechanisms_of_protein_targeting_to_leaf_lipid_droplets_ L2 - https://doi.org/10.1111/j.1744-7909.2011.01077.x DB - PRIME DP - Unbound Medicine ER -