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Granulocyte colony stimulating factor neuroprotective effects on spinal motoneurons after ventral root avulsion.
Synapse. 2012 Feb; 66(2):128-41.S

Abstract

G-CSF is a glycoprotein commonly used to treat neutropenia. Recent studies have shown that the G-CSF receptor (G-CSF-R) is expressed by neurons in the central nervous system (CNS), and neuroprotective effects of G-CSF have been observed. In this study, the influence of G-CSF treatment on the glial reactivity and synaptic plasticity of spinal motoneurons in rats subjected to ventral root avulsion (VRA) was investigated. Lewis rats (7 weeks old) were subjected to unilateral VRA and divided into two groups: G-CSF and placebo treated. The drug treated animals were injected subcutaneously with 200 μg/kg/day of G-CSF for 5 days post lesion. The placebo group received saline buffer. After 2 weeks, both groups were sacrificed and their lumbar intumescences processed for transmission electron microscopy (TEM), motoneuron counting, and immunohistochemistry with antibodies against GFAP, Iba-1, and synaptophysin. Furthermore, in vitro analysis was carried out, using newborn cortical derived astrocytes. The results indicated increased neuronal survival in the G-CSF treated group coupled with synaptic preservation. TEM analyses revealed an improved preservation of the synaptic covering in treated animals. Additionally, the drug treated group showed an increase in astroglial reactivity both in vivo and in vitro. The astrocytes also presented an increased cell proliferation rate when compared with the controls after 3 days of culturing. In conclusion, the present results suggest that G-CSF has an influence on the stability of presynaptic terminals in the spinal cord as well as on the astroglial reaction, indicating a possible neuroprotective action.

Authors+Show Affiliations

Department of Structural and Functional Biology, Institute of Biology, University of Campinas, Campinas, SP, Brazil. camilafreria@yahoo.comNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21953623

Citation

De Freria, Camila Marques, et al. "Granulocyte Colony Stimulating Factor Neuroprotective Effects On Spinal Motoneurons After Ventral Root Avulsion." Synapse (New York, N.Y.), vol. 66, no. 2, 2012, pp. 128-41.
De Freria CM, Barbizan R, De Oliveira AL. Granulocyte colony stimulating factor neuroprotective effects on spinal motoneurons after ventral root avulsion. Synapse. 2012;66(2):128-41.
De Freria, C. M., Barbizan, R., & De Oliveira, A. L. (2012). Granulocyte colony stimulating factor neuroprotective effects on spinal motoneurons after ventral root avulsion. Synapse (New York, N.Y.), 66(2), 128-41. https://doi.org/10.1002/syn.20993
De Freria CM, Barbizan R, De Oliveira AL. Granulocyte Colony Stimulating Factor Neuroprotective Effects On Spinal Motoneurons After Ventral Root Avulsion. Synapse. 2012;66(2):128-41. PubMed PMID: 21953623.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Granulocyte colony stimulating factor neuroprotective effects on spinal motoneurons after ventral root avulsion. AU - De Freria,Camila Marques, AU - Barbizan,Roberta, AU - De Oliveira,Alexandre Leite Rodrigues, Y1 - 2011/11/03/ PY - 2011/08/16/received PY - 2011/09/14/accepted PY - 2011/9/29/entrez PY - 2011/9/29/pubmed PY - 2012/8/23/medline SP - 128 EP - 41 JF - Synapse (New York, N.Y.) JO - Synapse VL - 66 IS - 2 N2 - G-CSF is a glycoprotein commonly used to treat neutropenia. Recent studies have shown that the G-CSF receptor (G-CSF-R) is expressed by neurons in the central nervous system (CNS), and neuroprotective effects of G-CSF have been observed. In this study, the influence of G-CSF treatment on the glial reactivity and synaptic plasticity of spinal motoneurons in rats subjected to ventral root avulsion (VRA) was investigated. Lewis rats (7 weeks old) were subjected to unilateral VRA and divided into two groups: G-CSF and placebo treated. The drug treated animals were injected subcutaneously with 200 μg/kg/day of G-CSF for 5 days post lesion. The placebo group received saline buffer. After 2 weeks, both groups were sacrificed and their lumbar intumescences processed for transmission electron microscopy (TEM), motoneuron counting, and immunohistochemistry with antibodies against GFAP, Iba-1, and synaptophysin. Furthermore, in vitro analysis was carried out, using newborn cortical derived astrocytes. The results indicated increased neuronal survival in the G-CSF treated group coupled with synaptic preservation. TEM analyses revealed an improved preservation of the synaptic covering in treated animals. Additionally, the drug treated group showed an increase in astroglial reactivity both in vivo and in vitro. The astrocytes also presented an increased cell proliferation rate when compared with the controls after 3 days of culturing. In conclusion, the present results suggest that G-CSF has an influence on the stability of presynaptic terminals in the spinal cord as well as on the astroglial reaction, indicating a possible neuroprotective action. SN - 1098-2396 UR - https://www.unboundmedicine.com/medline/citation/21953623/Granulocyte_colony_stimulating_factor_neuroprotective_effects_on_spinal_motoneurons_after_ventral_root_avulsion_ L2 - https://doi.org/10.1002/syn.20993 DB - PRIME DP - Unbound Medicine ER -