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In vitro antioxidant and radical-scavenging capacities of Citrullus colocynthes (L) and Artemisia absinthium extracts using promethazine hydrochloride radical cation and contemporary assays.
Food Sci Technol Int. 2011 Oct; 17(5):481-94.FS

Abstract

A new, quick and economical decolorization assay based upon the generation of a radical cation made from promethazine hydrochloride (PMZH) is described for screening of antioxidant activity of plants/herbal extracts. PMZH radical cations, produced through a reaction between PMZH and potassium persulfate (K(2)S(2)O(8)) in phosphoric acid medium, have maximum absorption at 515 nm in their first-order derivative spectrum. Theconcentrations of chromagen and K(2)S(2)O(8) were optimized (final concentration of PMZH and K2S2O8 were 0.166 mM and 0.11 mM, respectively) for better stability and sensitivity of the radical cation produced. Agood linear correlation was found between the percentage inhibition and the increasing amounts of standard antioxidants, with correlation coefficients ranging from 0.989 to 0.999. The newly developed assay was employed to evaluate the antioxidant capacity of Citrullus colocynthes L. and Artemisia absinthium extracts. The proposed assay involved a more stable radical cation and required only 1 h for preparation of a working solution in comparison to the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorizaion assay, which was reported to be less sensitive at low pH and almost 12-16 h were required for preparation of a working ABTS solution. Other assays employed to evaluate the antioxidant potential andradical-scavenging capacities of the extracts were the ferric-reducing antioxidant power, 2,2'-diphenyl-1-picrylhydrazyl radical scavenging, total phenolic contents assay, total flavonoid contents and metal-chelating activity assays, and the lipid peroxidation value in linoleic acid emulsion systems. The results indicate that boththe plants have potent free radical-scavenging activity and the ability to prevent lipid peroxidation and radical chain reactions.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Asghar MN
Department of Chemistry, Faculty of Science and Technology, GC University, Lahore-54000, Pakistan. mnasghar2003@yahoo.com
Khan IU
No affiliation info available
Bano N
No affiliation info available

MeSH

AntioxidantsArtemisia absinthiumCitrullusDrug DiscoveryFlavonoidsFree Radical ScavengersFruitIndiaIndicators and ReagentsIron Chelating AgentsMedicine, TraditionalPakistanPhenolsPlant ExtractsPlant LeavesPlant ShootsPotassium CompoundsPromethazineSolventsSpectrophotometrySulfates

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21954313

Citation

Asghar, M Nadeem, et al. "In Vitro Antioxidant and Radical-scavenging Capacities of Citrullus Colocynthes (L) and Artemisia Absinthium Extracts Using Promethazine Hydrochloride Radical Cation and Contemporary Assays." Food Science and Technology International = Ciencia Y Tecnologia De Los Alimentos Internacional, vol. 17, no. 5, 2011, pp. 481-94.
Asghar MN, Khan IU, Bano N. In vitro antioxidant and radical-scavenging capacities of Citrullus colocynthes (L) and Artemisia absinthium extracts using promethazine hydrochloride radical cation and contemporary assays. Food Sci Technol Int. 2011;17(5):481-94.
Asghar, M. N., Khan, I. U., & Bano, N. (2011). In vitro antioxidant and radical-scavenging capacities of Citrullus colocynthes (L) and Artemisia absinthium extracts using promethazine hydrochloride radical cation and contemporary assays. Food Science and Technology International = Ciencia Y Tecnologia De Los Alimentos Internacional, 17(5), 481-94. https://doi.org/10.1177/1082013211399495
Asghar MN, Khan IU, Bano N. In Vitro Antioxidant and Radical-scavenging Capacities of Citrullus Colocynthes (L) and Artemisia Absinthium Extracts Using Promethazine Hydrochloride Radical Cation and Contemporary Assays. Food Sci Technol Int. 2011;17(5):481-94. PubMed PMID: 21954313.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antioxidant and radical-scavenging capacities of Citrullus colocynthes (L) and Artemisia absinthium extracts using promethazine hydrochloride radical cation and contemporary assays. AU - Asghar,M Nadeem, AU - Khan,I Ullah, AU - Bano,N, Y1 - 2011/09/27/ PY - 2011/9/29/entrez PY - 2011/9/29/pubmed PY - 2011/12/15/medline SP - 481 EP - 94 JF - Food science and technology international = Ciencia y tecnologia de los alimentos internacional JO - Food Sci Technol Int VL - 17 IS - 5 N2 - A new, quick and economical decolorization assay based upon the generation of a radical cation made from promethazine hydrochloride (PMZH) is described for screening of antioxidant activity of plants/herbal extracts. PMZH radical cations, produced through a reaction between PMZH and potassium persulfate (K(2)S(2)O(8)) in phosphoric acid medium, have maximum absorption at 515 nm in their first-order derivative spectrum. Theconcentrations of chromagen and K(2)S(2)O(8) were optimized (final concentration of PMZH and K2S2O8 were 0.166 mM and 0.11 mM, respectively) for better stability and sensitivity of the radical cation produced. Agood linear correlation was found between the percentage inhibition and the increasing amounts of standard antioxidants, with correlation coefficients ranging from 0.989 to 0.999. The newly developed assay was employed to evaluate the antioxidant capacity of Citrullus colocynthes L. and Artemisia absinthium extracts. The proposed assay involved a more stable radical cation and required only 1 h for preparation of a working solution in comparison to the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical cation decolorizaion assay, which was reported to be less sensitive at low pH and almost 12-16 h were required for preparation of a working ABTS solution. Other assays employed to evaluate the antioxidant potential andradical-scavenging capacities of the extracts were the ferric-reducing antioxidant power, 2,2'-diphenyl-1-picrylhydrazyl radical scavenging, total phenolic contents assay, total flavonoid contents and metal-chelating activity assays, and the lipid peroxidation value in linoleic acid emulsion systems. The results indicate that boththe plants have potent free radical-scavenging activity and the ability to prevent lipid peroxidation and radical chain reactions. SN - 1082-0132 UR - https://www.unboundmedicine.com/medline/citation/21954313/In_vitro_antioxidant_and_radical_scavenging_capacities_of_Citrullus_colocynthes__L__and_Artemisia_absinthium_extracts_using_promethazine_hydrochloride_radical_cation_and_contemporary_assays_ DB - PRIME DP - Unbound Medicine ER -