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Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum.
Arch Oral Biol. 2012 Apr; 57(4):364-8.AO

Abstract

OBJECTIVE

To investigate the antibacterial activity of povidone-iodine (PVP-I) on an artificial dual species biofilm of periodontal pathogens.

DESIGN

Porphyromonas gingivalis or Fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (PCM) tissue culture inserts. After incubation for 72 h, PVP-I solutions were applied to the biofilm for the time period ranging from 0.5 to 5 min. After addition of a deactivator, each PCM was removed and the biofilm on the PCM was serially diluted and plated on blood agar plates and cultured anaerobically for 7 days. Then viable bacteria were enumerated.

RESULTS

In the dual species biofilm model, F. nucleatum showed an approximately 200-fold increase in viable counts when compared with mono-microbial biofilm. In dual species biofilm, PVP-I with concentration equal to or greater than 2% was required to significantly reduce P. gingivalis and F. nucleatum. When the contact time of PVP-I was increased to 1 min or greater, no difference in antibacterial activity of PVP-I was observed in any concentration.

CONCLUSION

These results suggest that 30s application of 2% PVP-I would be effective in suppressing both P. gingivalis and F. nucleatum in dual-species biofilm, and this provides clinical implication for the control of subgingival biofilm.

Authors+Show Affiliations

Department of Dentistry and Oral Surgery, School of Medicine, Keio University, 35 Shinanomachi, Sinnjuku-ku, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21958970

Citation

Hosaka, Yasuo, et al. "Antibacterial Activity of Povidone-iodine Against an Artificial Biofilm of Porphyromonas Gingivalis and Fusobacterium Nucleatum." Archives of Oral Biology, vol. 57, no. 4, 2012, pp. 364-8.
Hosaka Y, Saito A, Maeda R, et al. Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum. Arch Oral Biol. 2012;57(4):364-8.
Hosaka, Y., Saito, A., Maeda, R., Fukaya, C., Morikawa, S., Makino, A., Ishihara, K., & Nakagawa, T. (2012). Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum. Archives of Oral Biology, 57(4), 364-8. https://doi.org/10.1016/j.archoralbio.2011.09.005
Hosaka Y, et al. Antibacterial Activity of Povidone-iodine Against an Artificial Biofilm of Porphyromonas Gingivalis and Fusobacterium Nucleatum. Arch Oral Biol. 2012;57(4):364-8. PubMed PMID: 21958970.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antibacterial activity of povidone-iodine against an artificial biofilm of Porphyromonas gingivalis and Fusobacterium nucleatum. AU - Hosaka,Yasuo, AU - Saito,Atsushi, AU - Maeda,Ryo, AU - Fukaya,Chie, AU - Morikawa,Satoru, AU - Makino,Asako, AU - Ishihara,Kazuyuki, AU - Nakagawa,Taneaki, Y1 - 2011/09/28/ PY - 2011/08/01/received PY - 2011/08/22/revised PY - 2011/09/05/accepted PY - 2011/10/1/entrez PY - 2011/10/1/pubmed PY - 2012/8/3/medline SP - 364 EP - 8 JF - Archives of oral biology JO - Arch Oral Biol VL - 57 IS - 4 N2 - OBJECTIVE: To investigate the antibacterial activity of povidone-iodine (PVP-I) on an artificial dual species biofilm of periodontal pathogens. DESIGN: Porphyromonas gingivalis or Fusobacterium nucleatum grown in broth culture was inoculated on polycarbonate membrane (PCM) tissue culture inserts. After incubation for 72 h, PVP-I solutions were applied to the biofilm for the time period ranging from 0.5 to 5 min. After addition of a deactivator, each PCM was removed and the biofilm on the PCM was serially diluted and plated on blood agar plates and cultured anaerobically for 7 days. Then viable bacteria were enumerated. RESULTS: In the dual species biofilm model, F. nucleatum showed an approximately 200-fold increase in viable counts when compared with mono-microbial biofilm. In dual species biofilm, PVP-I with concentration equal to or greater than 2% was required to significantly reduce P. gingivalis and F. nucleatum. When the contact time of PVP-I was increased to 1 min or greater, no difference in antibacterial activity of PVP-I was observed in any concentration. CONCLUSION: These results suggest that 30s application of 2% PVP-I would be effective in suppressing both P. gingivalis and F. nucleatum in dual-species biofilm, and this provides clinical implication for the control of subgingival biofilm. SN - 1879-1506 UR - https://www.unboundmedicine.com/medline/citation/21958970/Antibacterial_activity_of_povidone_iodine_against_an_artificial_biofilm_of_Porphyromonas_gingivalis_and_Fusobacterium_nucleatum_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-9969(11)00284-6 DB - PRIME DP - Unbound Medicine ER -