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Identification of the dominant sulfate-reducing bacterial partner of anaerobic methanotrophs of the ANME-2 clade.
Environ Microbiol. 2010 Aug; 12(8):2327-40.EM

Abstract

The anaerobic oxidation of methane (AOM) with sulfate as terminal electron acceptor is mediated by consortia of methanotrophic archaea (ANME) and sulfate-reducing bacteria (SRB). Whereas three clades of ANME have been repeatedly studied with respect to phylogeny, key genes and genomic capabilities, little is known about their sulfate-reducing partner. In order to identify the partner of anaerobic methanotrophs of the ANME-2 clade, bacterial 16S rRNA gene libraries were constructed from cultures highly enriched for ANME-2a and ANME-2c in consortia with Deltaproteobacteria of the Desulfosarcina/Desulfococcus group (DSS). Phylogenetic analysis of those and publicly available sequences from AOM sites supported the hypothesis by Knittel and colleagues that the DSS partner belongs to the diverse SEEP-SRB1 cluster. Six subclusters of SEEP-SRB1, SEEP-SRB1a to SEEP-SRB1f, were proposed and specific oligonucleotide probes were designed. Using fluorescence in situ hybridization on samples from six different AOM sites, SEEP-SRB1a was identified as sulfate-reducing partner in up to 95% of total ANME-2 consortia. SEEP-SRB1a cells exhibited a rod-shaped, vibrioid, or coccoid morphology and were found to be associated with subgroups ANME-2a and ANME-2c. Moreover, SEEP-SRB1a was also detected in 8% to 23% of ANME-3 consortia in Haakon Mosby Mud Volcano sediments, previously described to be predominantly associated with SRB of the Desulfobulbus group. SEEP-SRB1a contributed to only 0.3% to 0.7% of all single cells in almost all samples indicating that these bacteria are highly adapted to a symbiotic relationship with ANME-2.

Authors+Show Affiliations

Max Planck Institute for Marine Microbiology, Celsiusstrasse 1, 28359 Bremen, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21966923

Citation

Schreiber, Lars, et al. "Identification of the Dominant Sulfate-reducing Bacterial Partner of Anaerobic Methanotrophs of the ANME-2 Clade." Environmental Microbiology, vol. 12, no. 8, 2010, pp. 2327-40.
Schreiber L, Holler T, Knittel K, et al. Identification of the dominant sulfate-reducing bacterial partner of anaerobic methanotrophs of the ANME-2 clade. Environ Microbiol. 2010;12(8):2327-40.
Schreiber, L., Holler, T., Knittel, K., Meyerdierks, A., & Amann, R. (2010). Identification of the dominant sulfate-reducing bacterial partner of anaerobic methanotrophs of the ANME-2 clade. Environmental Microbiology, 12(8), 2327-40. https://doi.org/10.1111/j.1462-2920.2010.02275.x
Schreiber L, et al. Identification of the Dominant Sulfate-reducing Bacterial Partner of Anaerobic Methanotrophs of the ANME-2 Clade. Environ Microbiol. 2010;12(8):2327-40. PubMed PMID: 21966923.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification of the dominant sulfate-reducing bacterial partner of anaerobic methanotrophs of the ANME-2 clade. AU - Schreiber,Lars, AU - Holler,Thomas, AU - Knittel,Katrin, AU - Meyerdierks,Anke, AU - Amann,Rudolf, Y1 - 2010/07/09/ PY - 2011/10/5/entrez PY - 2010/8/1/pubmed PY - 2011/12/14/medline SP - 2327 EP - 40 JF - Environmental microbiology JO - Environ. Microbiol. VL - 12 IS - 8 N2 - The anaerobic oxidation of methane (AOM) with sulfate as terminal electron acceptor is mediated by consortia of methanotrophic archaea (ANME) and sulfate-reducing bacteria (SRB). Whereas three clades of ANME have been repeatedly studied with respect to phylogeny, key genes and genomic capabilities, little is known about their sulfate-reducing partner. In order to identify the partner of anaerobic methanotrophs of the ANME-2 clade, bacterial 16S rRNA gene libraries were constructed from cultures highly enriched for ANME-2a and ANME-2c in consortia with Deltaproteobacteria of the Desulfosarcina/Desulfococcus group (DSS). Phylogenetic analysis of those and publicly available sequences from AOM sites supported the hypothesis by Knittel and colleagues that the DSS partner belongs to the diverse SEEP-SRB1 cluster. Six subclusters of SEEP-SRB1, SEEP-SRB1a to SEEP-SRB1f, were proposed and specific oligonucleotide probes were designed. Using fluorescence in situ hybridization on samples from six different AOM sites, SEEP-SRB1a was identified as sulfate-reducing partner in up to 95% of total ANME-2 consortia. SEEP-SRB1a cells exhibited a rod-shaped, vibrioid, or coccoid morphology and were found to be associated with subgroups ANME-2a and ANME-2c. Moreover, SEEP-SRB1a was also detected in 8% to 23% of ANME-3 consortia in Haakon Mosby Mud Volcano sediments, previously described to be predominantly associated with SRB of the Desulfobulbus group. SEEP-SRB1a contributed to only 0.3% to 0.7% of all single cells in almost all samples indicating that these bacteria are highly adapted to a symbiotic relationship with ANME-2. SN - 1462-2920 UR - https://www.unboundmedicine.com/medline/citation/21966923/Identification_of_the_dominant_sulfate_reducing_bacterial_partner_of_anaerobic_methanotrophs_of_the_ANME_2_clade_ L2 - https://doi.org/10.1111/j.1462-2920.2010.02275.x DB - PRIME DP - Unbound Medicine ER -