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In vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from the leaves of tropical medicinal plant Tetrastigma from Sabah.
Asian Pac J Trop Med. 2011 Sep; 4(9):717-21.AP

Abstract

OBJECTIVE

To detect the in vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from the leaves of tropical medicinal plant Tetrastigma from Sabah.

METHODS

The dry powder leaves of Tetrastigma were extracted with different organic solvent such as hexane, ethyl acetate, chloroform, butanol and aqueous methanol. The total phenolic and total flavonoids contents of the essential oil and various organic extracts such as hexane, ethyl acetate, chloroform, butanol and aqueous ethanol were determined by Folin - Ciocalteu method and the assayed antioxidant activity was determined in vitro models such as antioxidant capacity by radical scavenging activity using α, α-diphenyl- β-picrylhydrazyl (DPPH) method.

RESULTS

The total phenolic contents of the essential oil and different extracts as gallic acid equivalents were found to be highest in methanol extract (386.22 mg/g) followed by ethyl acetate (190.89 mg/g), chloroform (175.89 mg/g), hexane (173.44 mg/g), and butanol extract (131.72 mg/g) and the phenolic contents not detected in essential oil. The antioxidant capacity of the essential oil and different extracts as ascorbic acid standard was in the order of methanol extract > ethyl acetate extract >chloroform> butanol > hexane extract also the antioxidant activity was not detected in essential oil.

CONCLUSIONS

The findings show that the extent of antioxidant activity of the essential oil and all extracts are in accordance with the amount of phenolics present in that extract. Leaves of Tetrastigma being rich in phenolics may provide a good source of antioxidant.

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Authors+Show Affiliations

Hossain MA
Biotechnology Research Institute, Universiti Malaysia Sabah, Malaysia. waboponejosue@yahoo.fr
Shah MD
No affiliation info available
Gnanaraj C
No affiliation info available
Iqbal M
No affiliation info available

MeSH

AntioxidantsFlavonoidsMalaysiaOils, VolatilePhenolsPlant ExtractsPlant LeavesVitaceae

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21967695

Citation

Hossain, M Amzad, et al. "In Vitro Total Phenolics, Flavonoids Contents and Antioxidant Activity of Essential Oil, Various Organic Extracts From the Leaves of Tropical Medicinal Plant Tetrastigma From Sabah." Asian Pacific Journal of Tropical Medicine, vol. 4, no. 9, 2011, pp. 717-21.
Hossain MA, Shah MD, Gnanaraj C, et al. In vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from the leaves of tropical medicinal plant Tetrastigma from Sabah. Asian Pac J Trop Med. 2011;4(9):717-21.
Hossain, M. A., Shah, M. D., Gnanaraj, C., & Iqbal, M. (2011). In vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from the leaves of tropical medicinal plant Tetrastigma from Sabah. Asian Pacific Journal of Tropical Medicine, 4(9), 717-21. https://doi.org/10.1016/S1995-7645(11)60180-6
Hossain MA, et al. In Vitro Total Phenolics, Flavonoids Contents and Antioxidant Activity of Essential Oil, Various Organic Extracts From the Leaves of Tropical Medicinal Plant Tetrastigma From Sabah. Asian Pac J Trop Med. 2011;4(9):717-21. PubMed PMID: 21967695.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from the leaves of tropical medicinal plant Tetrastigma from Sabah. AU - Hossain,M Amzad, AU - Shah,Muhammad Dawood, AU - Gnanaraj,Charles, AU - Iqbal,Muhammad, PY - 2011/05/30/received PY - 2011/07/11/revised PY - 2011/08/15/accepted PY - 2011/10/5/entrez PY - 2011/10/5/pubmed PY - 2012/1/24/medline SP - 717 EP - 21 JF - Asian Pacific journal of tropical medicine JO - Asian Pac J Trop Med VL - 4 IS - 9 N2 - OBJECTIVE: To detect the in vitro total phenolics, flavonoids contents and antioxidant activity of essential oil, various organic extracts from the leaves of tropical medicinal plant Tetrastigma from Sabah. METHODS: The dry powder leaves of Tetrastigma were extracted with different organic solvent such as hexane, ethyl acetate, chloroform, butanol and aqueous methanol. The total phenolic and total flavonoids contents of the essential oil and various organic extracts such as hexane, ethyl acetate, chloroform, butanol and aqueous ethanol were determined by Folin - Ciocalteu method and the assayed antioxidant activity was determined in vitro models such as antioxidant capacity by radical scavenging activity using α, α-diphenyl- β-picrylhydrazyl (DPPH) method. RESULTS: The total phenolic contents of the essential oil and different extracts as gallic acid equivalents were found to be highest in methanol extract (386.22 mg/g) followed by ethyl acetate (190.89 mg/g), chloroform (175.89 mg/g), hexane (173.44 mg/g), and butanol extract (131.72 mg/g) and the phenolic contents not detected in essential oil. The antioxidant capacity of the essential oil and different extracts as ascorbic acid standard was in the order of methanol extract > ethyl acetate extract >chloroform> butanol > hexane extract also the antioxidant activity was not detected in essential oil. CONCLUSIONS: The findings show that the extent of antioxidant activity of the essential oil and all extracts are in accordance with the amount of phenolics present in that extract. Leaves of Tetrastigma being rich in phenolics may provide a good source of antioxidant. SN - 2352-4146 UR - https://www.unboundmedicine.com/medline/citation/21967695/In_vitro_total_phenolics_flavonoids_contents_and_antioxidant_activity_of_essential_oil_various_organic_extracts_from_the_leaves_of_tropical_medicinal_plant_Tetrastigma_from_Sabah_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1995-7645(11)60180-6 DB - PRIME DP - Unbound Medicine ER -