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Down-regulated HS6ST2 in osteoarthritis and Kashin-Beck disease inhibits cell viability and influences expression of the genes relevant to aggrecan metabolism of human chondrocytes.
Rheumatology (Oxford). 2011 Dec; 50(12):2176-86.R

Abstract

OBJECTIVE

Primary OA and Kashin-Beck disease (KBD) show similar pathological changes in articular cartilage. The objective was to screen differentially expressed genes between OA and normal cartilage, confirm the candidate gene expression among OA, KBD and normal cartilage, and then clarify its role in vitro.

METHODS

Differentially expressed genes in OA cartilage were screened by suppression subtractive hybridization (SSH) and verified by real-time quantitative PCR (Q-PCR) analysis. Heparan sulphate 6-O-sulphotransferase 2 (HS6ST2) expression was identified by Q-PCR and immunohistochemistry. After suppressing HS6ST2 by RNA interference in C28/I2 human chondrocyte line, the effects were analysed through determining the cell viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), the aggrecan contents by toluidine blue staining and the mRNA expression levels of SRY-type high mobility group box 9 (SOX9), AGC1, MMP3, a disintegrin and metalloproteinase domain with thrombospondin motifs 4 (ADAMTS4) and ADAMTS5 by Q-PCR.

RESULTS

HS6ST2 in the reverse subtraction library was identified as a down-regulated gene in OA and KBD at both mRNA and protein levels. The percentage of safranion O staining area was correlated positively with the percentage of HS6ST2-positive chondrocytes in OA and KBD cartilage. After HS6ST2-specific short interfering RNA (siRNA) transfection to C28/I2 cells, the cell viability was inhibited significantly, and the mRNA expression levels of SOX9 and AGC1 were reduced markedly, while MMP3 expression was increased significantly. CONCLUSION; HS6ST2 down-regulation was identified in both OA and KBD cartilage. The findings first suggest that HS6ST2 may participate in the pathogenesis of OA and KBD by influencing aggrecan metabolism.

Authors+Show Affiliations

Department of Genetics and Molecular Biology, Xi'an Jiaotong University College of Medicine, Xi'an, Shaanxi, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21972422

Citation

Wang, Wei, et al. "Down-regulated HS6ST2 in Osteoarthritis and Kashin-Beck Disease Inhibits Cell Viability and Influences Expression of the Genes Relevant to Aggrecan Metabolism of Human Chondrocytes." Rheumatology (Oxford, England), vol. 50, no. 12, 2011, pp. 2176-86.
Wang W, Zhong B, Sun J, et al. Down-regulated HS6ST2 in osteoarthritis and Kashin-Beck disease inhibits cell viability and influences expression of the genes relevant to aggrecan metabolism of human chondrocytes. Rheumatology (Oxford). 2011;50(12):2176-86.
Wang, W., Zhong, B., Sun, J., Cao, J., Tian, J., Zhong, N., Zhao, W., Tian, L., Xu, P., Guo, D., Ju, X., Ma, W., Li, M., Hou, W., & Lu, S. (2011). Down-regulated HS6ST2 in osteoarthritis and Kashin-Beck disease inhibits cell viability and influences expression of the genes relevant to aggrecan metabolism of human chondrocytes. Rheumatology (Oxford, England), 50(12), 2176-86. https://doi.org/10.1093/rheumatology/ker230
Wang W, et al. Down-regulated HS6ST2 in Osteoarthritis and Kashin-Beck Disease Inhibits Cell Viability and Influences Expression of the Genes Relevant to Aggrecan Metabolism of Human Chondrocytes. Rheumatology (Oxford). 2011;50(12):2176-86. PubMed PMID: 21972422.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Down-regulated HS6ST2 in osteoarthritis and Kashin-Beck disease inhibits cell viability and influences expression of the genes relevant to aggrecan metabolism of human chondrocytes. AU - Wang,Wei, AU - Zhong,Bo, AU - Sun,Jian, AU - Cao,Junling, AU - Tian,Juan, AU - Zhong,Nannan, AU - Zhao,Wenxiang, AU - Tian,Lifang, AU - Xu,Peng, AU - Guo,Dawei, AU - Ju,Xichi, AU - Ma,Wei, AU - Li,Meng, AU - Hou,Weikun, AU - Lu,Shemin, Y1 - 2011/10/04/ PY - 2011/10/6/entrez PY - 2011/10/6/pubmed PY - 2012/1/13/medline SP - 2176 EP - 86 JF - Rheumatology (Oxford, England) JO - Rheumatology (Oxford) VL - 50 IS - 12 N2 - OBJECTIVE: Primary OA and Kashin-Beck disease (KBD) show similar pathological changes in articular cartilage. The objective was to screen differentially expressed genes between OA and normal cartilage, confirm the candidate gene expression among OA, KBD and normal cartilage, and then clarify its role in vitro. METHODS: Differentially expressed genes in OA cartilage were screened by suppression subtractive hybridization (SSH) and verified by real-time quantitative PCR (Q-PCR) analysis. Heparan sulphate 6-O-sulphotransferase 2 (HS6ST2) expression was identified by Q-PCR and immunohistochemistry. After suppressing HS6ST2 by RNA interference in C28/I2 human chondrocyte line, the effects were analysed through determining the cell viability by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), the aggrecan contents by toluidine blue staining and the mRNA expression levels of SRY-type high mobility group box 9 (SOX9), AGC1, MMP3, a disintegrin and metalloproteinase domain with thrombospondin motifs 4 (ADAMTS4) and ADAMTS5 by Q-PCR. RESULTS: HS6ST2 in the reverse subtraction library was identified as a down-regulated gene in OA and KBD at both mRNA and protein levels. The percentage of safranion O staining area was correlated positively with the percentage of HS6ST2-positive chondrocytes in OA and KBD cartilage. After HS6ST2-specific short interfering RNA (siRNA) transfection to C28/I2 cells, the cell viability was inhibited significantly, and the mRNA expression levels of SOX9 and AGC1 were reduced markedly, while MMP3 expression was increased significantly. CONCLUSION; HS6ST2 down-regulation was identified in both OA and KBD cartilage. The findings first suggest that HS6ST2 may participate in the pathogenesis of OA and KBD by influencing aggrecan metabolism. SN - 1462-0332 UR - https://www.unboundmedicine.com/medline/citation/21972422/Down_regulated_HS6ST2_in_osteoarthritis_and_Kashin_Beck_disease_inhibits_cell_viability_and_influences_expression_of_the_genes_relevant_to_aggrecan_metabolism_of_human_chondrocytes_ L2 - https://academic.oup.com/rheumatology/article-lookup/doi/10.1093/rheumatology/ker230 DB - PRIME DP - Unbound Medicine ER -