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Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC.
J Clin Microbiol. 2011 Dec; 49(12):4299-300.JC

Abstract

A multiplex PCR method was developed for the detection of Clostridium difficile toxin genes tcdA, tcdB, ctdA, and cdtB and the major in-frame deletion types (18, 39, and 54 bp) of tcdC. The method has high specificity for PCR ribotype 027 and may identify other C. difficile strains of clinical and epidemiological importance.

Authors+Show Affiliations

Department of Microbiological Diagnostics, The National Reference Laboratory for Enteropathogenic Bacteria, Unit of Gastrointestinal Infections, Statens Serum Institut, Ørestads Boulevard 5, 2300 Copenhagen S, Denmark. SPN@ssi.dkNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article

Language

eng

PubMed ID

21976756

Citation

Persson, Søren, et al. "Multiplex PCR Method for Detection of Clostridium Difficile tcdA, tcdB, cdtA, and cdtB and Internal In-frame Deletion of TcdC." Journal of Clinical Microbiology, vol. 49, no. 12, 2011, pp. 4299-300.
Persson S, Jensen JN, Olsen KE. Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC. J Clin Microbiol. 2011;49(12):4299-300.
Persson, S., Jensen, J. N., & Olsen, K. E. (2011). Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC. Journal of Clinical Microbiology, 49(12), 4299-300. https://doi.org/10.1128/JCM.05161-11
Persson S, Jensen JN, Olsen KE. Multiplex PCR Method for Detection of Clostridium Difficile tcdA, tcdB, cdtA, and cdtB and Internal In-frame Deletion of TcdC. J Clin Microbiol. 2011;49(12):4299-300. PubMed PMID: 21976756.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Multiplex PCR method for detection of Clostridium difficile tcdA, tcdB, cdtA, and cdtB and internal in-frame deletion of tcdC. AU - Persson,Søren, AU - Jensen,Joan N, AU - Olsen,Katharina E P, Y1 - 2011/10/05/ PY - 2011/10/7/entrez PY - 2011/10/7/pubmed PY - 2012/3/2/medline SP - 4299 EP - 300 JF - Journal of clinical microbiology JO - J Clin Microbiol VL - 49 IS - 12 N2 - A multiplex PCR method was developed for the detection of Clostridium difficile toxin genes tcdA, tcdB, ctdA, and cdtB and the major in-frame deletion types (18, 39, and 54 bp) of tcdC. The method has high specificity for PCR ribotype 027 and may identify other C. difficile strains of clinical and epidemiological importance. SN - 1098-660X UR - https://www.unboundmedicine.com/medline/citation/21976756/Multiplex_PCR_method_for_detection_of_Clostridium_difficile_tcdA_tcdB_cdtA_and_cdtB_and_internal_in_frame_deletion_of_tcdC_ L2 - http://jcm.asm.org/cgi/pmidlookup?view=long&pmid=21976756 DB - PRIME DP - Unbound Medicine ER -