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Ethanol production from xylose by a recombinant Candida utilis strain expressing protein-engineered xylose reductase and xylitol dehydrogenase.
Biosci Biotechnol Biochem. 2011; 75(10):1994-2000.BB

Abstract

The industrial yeast Candida utilis can grow on media containing xylose as sole carbon source, but cannot ferment it to ethanol. The deficiency might be due to the low activity of NADPH-preferring xylose reductase (XR) and NAD(+)-dependent xylitol dehydogenase (XDH), which convert xylose to xylulose, because C. utilis can ferment xylulose. We introduced multiple site-directed mutations in the coenzyme binding sites of XR and XDH derived from the xylose-fermenting yeast Candida shehatae to alter their coenzyme specificities. Several combinations of recombinant and native XRs and XDHs were tested. Highest productivity was observed in a strain expressing CsheXR K275R/N277D (NADH-preferring) and native CsheXDH (NAD(+)-dependent), which produced 17.4 g/L of ethanol from 50 g/L of xylose in 20 h. Analysis of the genes responsible for ethanol production from the xylose capacity of C. utilis indicated that the introduction of CsheXDH was essential, while overexpression of CsheXR K275R/N277D improved efficiency of ethanol production.

Authors+Show Affiliations

Central Laboratories for Frontier Technology, KIRIN Holdings Co., Ltd., Yokohama, Kanagawa, Japan. Hideyuki.Tamakawa@kirin.co.jpNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

21979076

Citation

Tamakawa, Hideyuki, et al. "Ethanol Production From Xylose By a Recombinant Candida Utilis Strain Expressing Protein-engineered Xylose Reductase and Xylitol Dehydrogenase." Bioscience, Biotechnology, and Biochemistry, vol. 75, no. 10, 2011, pp. 1994-2000.
Tamakawa H, Ikushima S, Yoshida S. Ethanol production from xylose by a recombinant Candida utilis strain expressing protein-engineered xylose reductase and xylitol dehydrogenase. Biosci Biotechnol Biochem. 2011;75(10):1994-2000.
Tamakawa, H., Ikushima, S., & Yoshida, S. (2011). Ethanol production from xylose by a recombinant Candida utilis strain expressing protein-engineered xylose reductase and xylitol dehydrogenase. Bioscience, Biotechnology, and Biochemistry, 75(10), 1994-2000.
Tamakawa H, Ikushima S, Yoshida S. Ethanol Production From Xylose By a Recombinant Candida Utilis Strain Expressing Protein-engineered Xylose Reductase and Xylitol Dehydrogenase. Biosci Biotechnol Biochem. 2011;75(10):1994-2000. PubMed PMID: 21979076.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Ethanol production from xylose by a recombinant Candida utilis strain expressing protein-engineered xylose reductase and xylitol dehydrogenase. AU - Tamakawa,Hideyuki, AU - Ikushima,Shigehito, AU - Yoshida,Satoshi, Y1 - 2011/10/07/ PY - 2011/10/8/entrez PY - 2011/10/8/pubmed PY - 2012/2/15/medline SP - 1994 EP - 2000 JF - Bioscience, biotechnology, and biochemistry JO - Biosci Biotechnol Biochem VL - 75 IS - 10 N2 - The industrial yeast Candida utilis can grow on media containing xylose as sole carbon source, but cannot ferment it to ethanol. The deficiency might be due to the low activity of NADPH-preferring xylose reductase (XR) and NAD(+)-dependent xylitol dehydogenase (XDH), which convert xylose to xylulose, because C. utilis can ferment xylulose. We introduced multiple site-directed mutations in the coenzyme binding sites of XR and XDH derived from the xylose-fermenting yeast Candida shehatae to alter their coenzyme specificities. Several combinations of recombinant and native XRs and XDHs were tested. Highest productivity was observed in a strain expressing CsheXR K275R/N277D (NADH-preferring) and native CsheXDH (NAD(+)-dependent), which produced 17.4 g/L of ethanol from 50 g/L of xylose in 20 h. Analysis of the genes responsible for ethanol production from the xylose capacity of C. utilis indicated that the introduction of CsheXDH was essential, while overexpression of CsheXR K275R/N277D improved efficiency of ethanol production. SN - 1347-6947 UR - https://www.unboundmedicine.com/medline/citation/21979076/Ethanol_production_from_xylose_by_a_recombinant_Candida_utilis_strain_expressing_protein_engineered_xylose_reductase_and_xylitol_dehydrogenase_ L2 - https://joi.jlc.jst.go.jp/JST.JSTAGE/bbb/110426?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -