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Interstitial cells of Cajal in the gastrointestinal musculature of W(jic) c-kit mutant mice.
J Smooth Muscle Res. 2011; 47(3-4):111-21.JS

Abstract

Interstitial cells of Cajal (ICC) generate electrical rhythmicity and transduce neural signals in the gastrointestinal musculature. ICC express the proto-oncogene c-kit, a receptor tyrosine kinase, and are identified morphologically by c-Kit immunoreactivity. The c-kit gene is allelic with the murine white-spotting locus W, and mutations of c-kit are known as W mutations. W mutations affect various developmental aspects of hematopoietic cells, germ cells, melanocytes, mast cells and ICC. We examined W(jic)/W(jic) mutant mice that have a mutation in the tyrosine kinase domain resulting in severe loss of protein function. W(jic)/W(jic) homozygotes exhibited white coats and black eyes. The gross morphology of the gastrointestinal tract showed no abnormality in mutant mice other than a forestomach papilloma. In the stomach, intramuscular ICC (ICC-IM) were missing, and myenteric ICC (ICC-MY) were reduced in number. In the small intestine, the number of ICC-MY was severely reduced; however there was a normal distribution of deep muscular plexus ICC (ICC-DMP). In the cecum, the numbers of ICC-IM and ICC-MY were severely depleted. ICC-IM were almost entirely absent in the colon, whereas ICC-MY loss was restricted to the distal colon. Patterns of ICC deficiency were generally similar between W(jic)/W(jic) mice and W/W(v) mutants, which lack a specific type of ICC. The enteric nervous system of the mutant mice appeared normal. From these findings, we conclude that W(jic)/W(jic) mice represent a distinct, novel genotype resulting in a lack of a specific type of ICC in the gastrointestinal musculature.

Authors+Show Affiliations

Department of Morphological and Physiological Sciences, University of Fukui, Japan. iinosa@u-fukui.ac.jpNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21979409

Citation

Iino, Satoshi, et al. "Interstitial Cells of Cajal in the Gastrointestinal Musculature of W(jic) C-kit Mutant Mice." Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi, vol. 47, no. 3-4, 2011, pp. 111-21.
Iino S, Horiguchi S, Horiguchi K. Interstitial cells of Cajal in the gastrointestinal musculature of W(jic) c-kit mutant mice. J Smooth Muscle Res. 2011;47(3-4):111-21.
Iino, S., Horiguchi, S., & Horiguchi, K. (2011). Interstitial cells of Cajal in the gastrointestinal musculature of W(jic) c-kit mutant mice. Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi, 47(3-4), 111-21.
Iino S, Horiguchi S, Horiguchi K. Interstitial Cells of Cajal in the Gastrointestinal Musculature of W(jic) C-kit Mutant Mice. J Smooth Muscle Res. 2011;47(3-4):111-21. PubMed PMID: 21979409.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Interstitial cells of Cajal in the gastrointestinal musculature of W(jic) c-kit mutant mice. AU - Iino,Satoshi, AU - Horiguchi,Satomi, AU - Horiguchi,Kazuhide, PY - 2011/10/8/entrez PY - 2011/10/8/pubmed PY - 2012/1/27/medline SP - 111 EP - 21 JF - Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi JO - J Smooth Muscle Res VL - 47 IS - 3-4 N2 - Interstitial cells of Cajal (ICC) generate electrical rhythmicity and transduce neural signals in the gastrointestinal musculature. ICC express the proto-oncogene c-kit, a receptor tyrosine kinase, and are identified morphologically by c-Kit immunoreactivity. The c-kit gene is allelic with the murine white-spotting locus W, and mutations of c-kit are known as W mutations. W mutations affect various developmental aspects of hematopoietic cells, germ cells, melanocytes, mast cells and ICC. We examined W(jic)/W(jic) mutant mice that have a mutation in the tyrosine kinase domain resulting in severe loss of protein function. W(jic)/W(jic) homozygotes exhibited white coats and black eyes. The gross morphology of the gastrointestinal tract showed no abnormality in mutant mice other than a forestomach papilloma. In the stomach, intramuscular ICC (ICC-IM) were missing, and myenteric ICC (ICC-MY) were reduced in number. In the small intestine, the number of ICC-MY was severely reduced; however there was a normal distribution of deep muscular plexus ICC (ICC-DMP). In the cecum, the numbers of ICC-IM and ICC-MY were severely depleted. ICC-IM were almost entirely absent in the colon, whereas ICC-MY loss was restricted to the distal colon. Patterns of ICC deficiency were generally similar between W(jic)/W(jic) mice and W/W(v) mutants, which lack a specific type of ICC. The enteric nervous system of the mutant mice appeared normal. From these findings, we conclude that W(jic)/W(jic) mice represent a distinct, novel genotype resulting in a lack of a specific type of ICC in the gastrointestinal musculature. SN - 1884-8796 UR - https://www.unboundmedicine.com/medline/citation/21979409/Interstitial_cells_of_Cajal_in_the_gastrointestinal_musculature_of_W_jic__c_kit_mutant_mice_ L2 - http://joi.jlc.jst.go.jp/JST.JSTAGE/jsmr/47.111?lang=en&from=PubMed DB - PRIME DP - Unbound Medicine ER -