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Co-oximetry in clinically healthy dogs and effects of time of post sampling on measurements.
J Small Anim Pract. 2011 Dec; 52(12):628-31.JS

Abstract

OBJECTIVES

Co-oximetry is a complex and valuable laboratory method that measures haemoglobin species and oxygenation status by multi-wavelength spectrophotometry. The purpose of this study was to establish reference intervals for clinically healthy dogs and to determine the effect of time of analyses and sex of animals on the accuracy of results.

METHODS

Blood was collected from 27 healthy adult dogs of various breeds and sex. Co-oximetry was performed on a CCX co-oximeter that measures eight haemoglobin and oxygen transport related parameters: carboxyhaemoglobin (COHb), deoxyhaemoglobin (HHb), oxyhaemoglobin (O(2)Hb), methaemoglobin (MetHb), total haemoglobin (tHb), oxygen saturation (SO(2)%), oxygen content (O(2)Ct) and oxygen capacity (O(2)Cap).

RESULTS

Results obtained after 2 and 4 hours were not significantly different from those obtained immediately after sampling. But after 48 hours, the results for total haemoglobin, oxygen saturation, oxyhaemoglobin, oxygen content and oxygen capacity were significantly lower, and carboxyhaemoglobin and deoxyhaemoglobin values were significantly higher than determination immediately after sampling. Gender had no significant impact on co-oximetry values.

CLINICAL SIGNIFICANCE

Co-oximetry offers several advantages compared with other methods, including ease of use, increased accuracy and greater differentiation among haemoglobin species.

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Authors+Show Affiliations

Kuleš J
Department of Chemistry and Biochemistry, Faculty of Veterinary Medicine, University of Zagreb, Heinzelova 55, 10 000 Zagreb, Croatia.
Mayer I
No affiliation info available
Rafaj RB
No affiliation info available
Matijatko V
No affiliation info available
Kiš I
No affiliation info available
Kučer N
No affiliation info available
Brkljačić M
No affiliation info available
Mrljak V
No affiliation info available

MeSH

AnimalsBlood Gas AnalysisDogsFemaleHemoglobinsMaleOximetryOxygenReference ValuesReproducibility of ResultsSensitivity and SpecificitySex FactorsTime Factors

Pub Type(s)

Journal Article
Validation Study

Language

eng

PubMed ID

21981712

Citation

Kuleš, J, et al. "Co-oximetry in Clinically Healthy Dogs and Effects of Time of Post Sampling On Measurements." The Journal of Small Animal Practice, vol. 52, no. 12, 2011, pp. 628-31.
Kuleš J, Mayer I, Rafaj RB, et al. Co-oximetry in clinically healthy dogs and effects of time of post sampling on measurements. J Small Anim Pract. 2011;52(12):628-31.
Kuleš, J., Mayer, I., Rafaj, R. B., Matijatko, V., Kiš, I., Kučer, N., Brkljačić, M., & Mrljak, V. (2011). Co-oximetry in clinically healthy dogs and effects of time of post sampling on measurements. The Journal of Small Animal Practice, 52(12), 628-31. https://doi.org/10.1111/j.1748-5827.2011.01129.x
Kuleš J, et al. Co-oximetry in Clinically Healthy Dogs and Effects of Time of Post Sampling On Measurements. J Small Anim Pract. 2011;52(12):628-31. PubMed PMID: 21981712.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Co-oximetry in clinically healthy dogs and effects of time of post sampling on measurements. AU - Kuleš,J, AU - Mayer,I, AU - Rafaj,R Barić, AU - Matijatko,V, AU - Kiš,I, AU - Kučer,N, AU - Brkljačić,M, AU - Mrljak,V, Y1 - 2011/10/07/ PY - 2011/10/11/entrez PY - 2011/10/11/pubmed PY - 2012/3/9/medline SP - 628 EP - 31 JF - The Journal of small animal practice JO - J Small Anim Pract VL - 52 IS - 12 N2 - OBJECTIVES: Co-oximetry is a complex and valuable laboratory method that measures haemoglobin species and oxygenation status by multi-wavelength spectrophotometry. The purpose of this study was to establish reference intervals for clinically healthy dogs and to determine the effect of time of analyses and sex of animals on the accuracy of results. METHODS: Blood was collected from 27 healthy adult dogs of various breeds and sex. Co-oximetry was performed on a CCX co-oximeter that measures eight haemoglobin and oxygen transport related parameters: carboxyhaemoglobin (COHb), deoxyhaemoglobin (HHb), oxyhaemoglobin (O(2)Hb), methaemoglobin (MetHb), total haemoglobin (tHb), oxygen saturation (SO(2)%), oxygen content (O(2)Ct) and oxygen capacity (O(2)Cap). RESULTS: Results obtained after 2 and 4 hours were not significantly different from those obtained immediately after sampling. But after 48 hours, the results for total haemoglobin, oxygen saturation, oxyhaemoglobin, oxygen content and oxygen capacity were significantly lower, and carboxyhaemoglobin and deoxyhaemoglobin values were significantly higher than determination immediately after sampling. Gender had no significant impact on co-oximetry values. CLINICAL SIGNIFICANCE: Co-oximetry offers several advantages compared with other methods, including ease of use, increased accuracy and greater differentiation among haemoglobin species. SN - 1748-5827 UR - https://www.unboundmedicine.com/medline/citation/21981712/Co_oximetry_in_clinically_healthy_dogs_and_effects_of_time_of_post_sampling_on_measurements_ L2 - https://doi.org/10.1111/j.1748-5827.2011.01129.x DB - PRIME DP - Unbound Medicine ER -