Tags

Type your tag names separated by a space and hit enter

Evidence of differences between the communities of arbuscular mycorrhizal fungi colonizing galls and roots of Prunus persica infected by the root-knot nematode Meloidogyne incognita.
Appl Environ Microbiol. 2011 Dec; 77(24):8656-61.AE

Abstract

Arbuscular mycorrhizal fungi (AMF) play important roles as plant protection agents, reducing or suppressing nematode colonization. However, it has never been investigated whether the galls produced in roots by nematode infection are colonized by AMF. This study tested whether galls produced by Meloidogyne incognita infection in Prunus persica roots are colonized by AMF. We also determined the changes in AMF composition and biodiversity mediated by infection with this root-knot nematode. DNA from galls and roots of plants infected by M. incognita and from roots of noninfected plants was extracted, amplified, cloned, and sequenced using AMF-specific primers. Phylogenetic analysis using the small-subunit (SSU) ribosomal DNA (rDNA) data set revealed 22 different AMF sequence types (17 Glomus sequence types, 3 Paraglomus sequence types, 1 Scutellospora sequence type, and 1 Acaulospora sequence type). The highest AMF diversity was found in uninfected roots, followed by infected roots and galls. This study indicates that the galls produced in P. persica roots due to infection with M. incognita were colonized extensively by a community of AMF, belonging to the families Paraglomeraceae and Glomeraceae, that was different from the community detected in roots. Although the function of the AMF in the galls is still unknown, we hypothesize that they act as protection agents against opportunistic pathogens.

Links

PMC Free PDF
PMC Free Full Text
Publisher Full Text

Authors+Show Affiliations

Alguacil Mdel M
CSIC-Centro de Edafología y Biología Aplicada del Segura, Department of Soil and Water Conservation, P.O. Box 164, Campus de Espinardo, 30100 Murcia, Spain. mmalguacil@cebas.csic.es
Torrecillas E
No affiliation info available
Lozano Z
No affiliation info available
Roldán A
No affiliation info available

MeSH

AnimalsBiotaCluster AnalysisDNA, FungalDNA, RibosomalFungiGenes, rRNAMolecular Sequence DataMycorrhizaePhylogenyPlant DiseasesPlant RootsPlant TumorsPrunusRNA, FungalRNA, Ribosomal, 18SSequence Analysis, DNATylenchoidea

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21984233

Citation

Alguacil, Maria del Mar, et al. "Evidence of Differences Between the Communities of Arbuscular Mycorrhizal Fungi Colonizing Galls and Roots of Prunus Persica Infected By the Root-knot Nematode Meloidogyne Incognita." Applied and Environmental Microbiology, vol. 77, no. 24, 2011, pp. 8656-61.
Alguacil Mdel M, Torrecillas E, Lozano Z, et al. Evidence of differences between the communities of arbuscular mycorrhizal fungi colonizing galls and roots of Prunus persica infected by the root-knot nematode Meloidogyne incognita. Appl Environ Microbiol. 2011;77(24):8656-61.
Alguacil, M. d. e. l. . M., Torrecillas, E., Lozano, Z., & Roldán, A. (2011). Evidence of differences between the communities of arbuscular mycorrhizal fungi colonizing galls and roots of Prunus persica infected by the root-knot nematode Meloidogyne incognita. Applied and Environmental Microbiology, 77(24), 8656-61. https://doi.org/10.1128/AEM.05577-11
Alguacil Mdel M, et al. Evidence of Differences Between the Communities of Arbuscular Mycorrhizal Fungi Colonizing Galls and Roots of Prunus Persica Infected By the Root-knot Nematode Meloidogyne Incognita. Appl Environ Microbiol. 2011;77(24):8656-61. PubMed PMID: 21984233.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evidence of differences between the communities of arbuscular mycorrhizal fungi colonizing galls and roots of Prunus persica infected by the root-knot nematode Meloidogyne incognita. AU - Alguacil,Maria del Mar, AU - Torrecillas,Emma, AU - Lozano,Zenaida, AU - Roldán,Antonio, Y1 - 2011/10/07/ PY - 2011/10/11/entrez PY - 2011/10/11/pubmed PY - 2012/3/22/medline SP - 8656 EP - 61 JF - Applied and environmental microbiology JO - Appl Environ Microbiol VL - 77 IS - 24 N2 - Arbuscular mycorrhizal fungi (AMF) play important roles as plant protection agents, reducing or suppressing nematode colonization. However, it has never been investigated whether the galls produced in roots by nematode infection are colonized by AMF. This study tested whether galls produced by Meloidogyne incognita infection in Prunus persica roots are colonized by AMF. We also determined the changes in AMF composition and biodiversity mediated by infection with this root-knot nematode. DNA from galls and roots of plants infected by M. incognita and from roots of noninfected plants was extracted, amplified, cloned, and sequenced using AMF-specific primers. Phylogenetic analysis using the small-subunit (SSU) ribosomal DNA (rDNA) data set revealed 22 different AMF sequence types (17 Glomus sequence types, 3 Paraglomus sequence types, 1 Scutellospora sequence type, and 1 Acaulospora sequence type). The highest AMF diversity was found in uninfected roots, followed by infected roots and galls. This study indicates that the galls produced in P. persica roots due to infection with M. incognita were colonized extensively by a community of AMF, belonging to the families Paraglomeraceae and Glomeraceae, that was different from the community detected in roots. Although the function of the AMF in the galls is still unknown, we hypothesize that they act as protection agents against opportunistic pathogens. SN - 1098-5336 UR - https://www.unboundmedicine.com/medline/citation/21984233/Evidence_of_differences_between_the_communities_of_arbuscular_mycorrhizal_fungi_colonizing_galls_and_roots_of_Prunus_persica_infected_by_the_root_knot_nematode_Meloidogyne_incognita_ L2 - https://journals.asm.org/doi/10.1128/AEM.05577-11?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -