Tags

Type your tag names separated by a space and hit enter

Association of human immune response to Aedes aegypti salivary proteins with dengue disease severity.
Parasite Immunol. 2012 Jan; 34(1):15-22.PI

Abstract

Dengue viruses (DENV; family Flaviviridae, genus Flavivirus) are transmitted by Aedes aegypti mosquitoes and can cause dengue fever (DF), a relatively benign disease, or more severe dengue haemorrhagic fever (DHF). Arthropod saliva contains proteins delivered into the bite wound that can modulate the host haemostatic and immune responses to facilitate the intake of a blood meal. The potential effects on DENV infection of previous exposure to Ae. aegypti salivary proteins have not been investigated. We collected Ae. aegypti saliva, concentrated the proteins and fractionated them by nondenaturing polyacrylamide gel electrophoresis (PAGE). By the use of immunoblots, we analysed reactivity with the mosquito salivary proteins (MSP) of sera from 96 Thai children diagnosed with secondary DENV infections leading either to DF or DHF, or with no DENV infection, and found that different proportions of each patient group had serum antibodies reactive to specific Ae. aegypti salivary proteins. Our results suggest that prior exposure to MSP might play a role in the outcome of DENV infection in humans.

Authors+Show Affiliations

Department of Microbiology, Immunology and Pathology, Arthropod-borne and Infectious Diseases Laboratory, Colorado State University, Fort Collins, CO, USA. carlos.machain@uady.mxNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

21995849

Citation

Machain-Williams, C, et al. "Association of Human Immune Response to Aedes Aegypti Salivary Proteins With Dengue Disease Severity." Parasite Immunology, vol. 34, no. 1, 2012, pp. 15-22.
Machain-Williams C, Mammen MP, Zeidner NS, et al. Association of human immune response to Aedes aegypti salivary proteins with dengue disease severity. Parasite Immunol. 2012;34(1):15-22.
Machain-Williams, C., Mammen, M. P., Zeidner, N. S., Beaty, B. J., Prenni, J. E., Nisalak, A., & Blair, C. D. (2012). Association of human immune response to Aedes aegypti salivary proteins with dengue disease severity. Parasite Immunology, 34(1), 15-22. https://doi.org/10.1111/j.1365-3024.2011.01339.x
Machain-Williams C, et al. Association of Human Immune Response to Aedes Aegypti Salivary Proteins With Dengue Disease Severity. Parasite Immunol. 2012;34(1):15-22. PubMed PMID: 21995849.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of human immune response to Aedes aegypti salivary proteins with dengue disease severity. AU - Machain-Williams,C, AU - Mammen,M P,Jr AU - Zeidner,N S, AU - Beaty,B J, AU - Prenni,J E, AU - Nisalak,A, AU - Blair,C D, PY - 2011/10/15/entrez PY - 2011/10/15/pubmed PY - 2012/4/5/medline SP - 15 EP - 22 JF - Parasite immunology JO - Parasite Immunol. VL - 34 IS - 1 N2 - Dengue viruses (DENV; family Flaviviridae, genus Flavivirus) are transmitted by Aedes aegypti mosquitoes and can cause dengue fever (DF), a relatively benign disease, or more severe dengue haemorrhagic fever (DHF). Arthropod saliva contains proteins delivered into the bite wound that can modulate the host haemostatic and immune responses to facilitate the intake of a blood meal. The potential effects on DENV infection of previous exposure to Ae. aegypti salivary proteins have not been investigated. We collected Ae. aegypti saliva, concentrated the proteins and fractionated them by nondenaturing polyacrylamide gel electrophoresis (PAGE). By the use of immunoblots, we analysed reactivity with the mosquito salivary proteins (MSP) of sera from 96 Thai children diagnosed with secondary DENV infections leading either to DF or DHF, or with no DENV infection, and found that different proportions of each patient group had serum antibodies reactive to specific Ae. aegypti salivary proteins. Our results suggest that prior exposure to MSP might play a role in the outcome of DENV infection in humans. SN - 1365-3024 UR - https://www.unboundmedicine.com/medline/citation/21995849/abstract/Association_of_Human_Immune_Response_to_Aedes_aegypti_Salivary_Proteins_with_Dengue_Disease_Severity_ L2 - https://doi.org/10.1111/j.1365-3024.2011.01339.x DB - PRIME DP - Unbound Medicine ER -