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Protective effects of baicalein against rotenone-induced neurotoxicity in PC12 cells and isolated rat brain mitochondria.
Eur J Pharmacol. 2012 Jan 15; 674(2-3):227-33.EJ

Abstract

Baicalein is one of the major flavonoids obtained from the Scutellaria root. Previous pharmacological studies found that baicalein had neuroprotective effects in animal models of Parkinson's disease. The purpose of this paper was to explore the molecular mechanism of the action of baicalein on PC12 cells and isolated rat brain mitochondria. Firstly, we investigated the effects of baicalein on rotenone-induced toxicity in PC12 cells. The results showed that baicalein suppressed rotenone-induced apoptosis, and inhibited the accumulation of reactive oxidant species, ATP deficiency, mitochondrial membrane potential dissipation, and caspase-3/7 activation in a concentration-dependent manner, indicating that baicalein likely improved mitochondrial function. Furthermore, we used isolated rat brain mitochondria to evaluate the effect of baicalein. Treatment with baicalein prevented rotenone-induced reactive oxidant species production, ATP deficiency and mitochondrial swelling in isolated brain mitochondria. Interestingly, exposure of isolated mitochondria to baicalein promoted mitochondrial active respiration. These results suggest that baicalein may be a mitochondria-targeted antioxidant and exerts neuroprotective effects on rotenone-induced neurotoxicity. This study supports our previous research that baicalein possesses neuroprotective activity in vivo and it is worthy of further study.

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Authors+Show Affiliations

Li XX
National Center for Pharmaceutical Screening, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, No. 1 Xian Nong Tan Street, Beijing 100050, PR China.
He GR
No affiliation info available
Mu X
No affiliation info available
Xu B
No affiliation info available
Tian S
No affiliation info available
Yu X
No affiliation info available
Meng FR
No affiliation info available
Xuan ZH
No affiliation info available
Du GH
No affiliation info available

MeSH

AnimalsApoptosisBrainCaspase 3Caspase 7Cell RespirationCytoprotectionFlavanonesMaleMembrane Potential, MitochondrialMitochondriaMitochondrial SwellingNeuroprotective AgentsNeurotoxinsOxidative StressPC12 CellsRatsRats, Sprague-DawleyRotenone

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

21996316

Citation

Li, Xiao-xiu, et al. "Protective Effects of Baicalein Against Rotenone-induced Neurotoxicity in PC12 Cells and Isolated Rat Brain Mitochondria." European Journal of Pharmacology, vol. 674, no. 2-3, 2012, pp. 227-33.
Li XX, He GR, Mu X, et al. Protective effects of baicalein against rotenone-induced neurotoxicity in PC12 cells and isolated rat brain mitochondria. Eur J Pharmacol. 2012;674(2-3):227-33.
Li, X. X., He, G. R., Mu, X., Xu, B., Tian, S., Yu, X., Meng, F. R., Xuan, Z. H., & Du, G. H. (2012). Protective effects of baicalein against rotenone-induced neurotoxicity in PC12 cells and isolated rat brain mitochondria. European Journal of Pharmacology, 674(2-3), 227-33. https://doi.org/10.1016/j.ejphar.2011.09.181
Li XX, et al. Protective Effects of Baicalein Against Rotenone-induced Neurotoxicity in PC12 Cells and Isolated Rat Brain Mitochondria. Eur J Pharmacol. 2012 Jan 15;674(2-3):227-33. PubMed PMID: 21996316.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protective effects of baicalein against rotenone-induced neurotoxicity in PC12 cells and isolated rat brain mitochondria. AU - Li,Xiao-xiu, AU - He,Guo-rong, AU - Mu,Xin, AU - Xu,Bei, AU - Tian,Shuo, AU - Yu,Xin, AU - Meng,Fan-rui, AU - Xuan,Zhao-hong, AU - Du,Guan-hua, Y1 - 2011/10/06/ PY - 2010/12/21/received PY - 2011/09/22/revised PY - 2011/09/24/accepted PY - 2011/10/15/entrez PY - 2011/10/15/pubmed PY - 2012/5/4/medline SP - 227 EP - 33 JF - European journal of pharmacology JO - Eur J Pharmacol VL - 674 IS - 2-3 N2 - Baicalein is one of the major flavonoids obtained from the Scutellaria root. Previous pharmacological studies found that baicalein had neuroprotective effects in animal models of Parkinson's disease. The purpose of this paper was to explore the molecular mechanism of the action of baicalein on PC12 cells and isolated rat brain mitochondria. Firstly, we investigated the effects of baicalein on rotenone-induced toxicity in PC12 cells. The results showed that baicalein suppressed rotenone-induced apoptosis, and inhibited the accumulation of reactive oxidant species, ATP deficiency, mitochondrial membrane potential dissipation, and caspase-3/7 activation in a concentration-dependent manner, indicating that baicalein likely improved mitochondrial function. Furthermore, we used isolated rat brain mitochondria to evaluate the effect of baicalein. Treatment with baicalein prevented rotenone-induced reactive oxidant species production, ATP deficiency and mitochondrial swelling in isolated brain mitochondria. Interestingly, exposure of isolated mitochondria to baicalein promoted mitochondrial active respiration. These results suggest that baicalein may be a mitochondria-targeted antioxidant and exerts neuroprotective effects on rotenone-induced neurotoxicity. This study supports our previous research that baicalein possesses neuroprotective activity in vivo and it is worthy of further study. SN - 1879-0712 UR - https://www.unboundmedicine.com/medline/citation/21996316/Protective_effects_of_baicalein_against_rotenone_induced_neurotoxicity_in_PC12_cells_and_isolated_rat_brain_mitochondria_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0014-2999(11)01164-2 DB - PRIME DP - Unbound Medicine ER -