Tags

Type your tag names separated by a space and hit enter

Screening of a polar extract of Paeonia rockii: composition and antioxidant and antifungal activities.
J Ethnopharmacol. 2011 Dec 08; 138(3):705-12.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The genus Paeonia (Paeoniaceae), is one of the most important source of crude drugs in traditional Chinese medicine and investigation on many species is large. Up to now studies on Paeonia rockii, one of the eight species recognized in the section Moutan, are very limited.

AIM OF THE STUDY

This research aimed to investigate the composition of Paeonia rockii roots and to evaluate the in vitro free-radical scavenging and antifungal activities of a polar extract (PPR) and its major constituents.

MATERIALS AND METHODS

PPR was obtained from defatted dried roots of Paeonia rockii using MeOH as extraction solvent. Its n-BuOH soluble portion (PPR-B) was purified by Sephadex LH-20 followed by RP-HPLC to give nineteen compounds belonging to the classes polyphenols, monoterpenes and triterpenes. Their structure were spectrally characterized (UV, 1D and 2D NMR, MS). The polyphenols content of PPR and PPR-B was examined by the Folin-Ciocalteau colorimetric assay and HPLC method. Both extracts (PPR and PPR-B) and their major constituents were tested for the free-radical scavenging activity by DPPH-test, and for the antifungal activity by three methods (micro-broth dilution method, XTT assay and Candida albicans morphological analysis).

RESULTS

5-Butylhydroxy-γ-lactone (1), and ethyl-arabinopyranoside (2) have been isolated for the first time as naturally occurring compounds and taxifolin (3) was reported for the first time in Paeonia spp. Nine polyphenols, four monoterpenes and three triterpenes were also identified. Both the extracts PPR and PPR-B had high polyphenol content, and high concentration of gallic acid derivatives and paeoniflorin, chemotaxonomic characteristic markers of the genus. PPR, gallic acid and methyl-gallate displayed high potency in scavenging free-radicals (DPPH test, EC(50) 13.3, 1.2, 1.9 μg/ml, respectively). Both the extracts and gallic acid individually showed an interesting antifungal property (MIC(50) at 24 h 25, 0.9 and 30 μg/ml, respectively) and notably, a combination of paeoniflorin/gallic acid (MIC(50)=0.5+20 μg/ml, respectively) was more active than the single compound in inhibiting Candida growth.

CONCLUSION

The polar methanolic extract (PPR), its n-BuOH soluble fraction and constituents of Paeonia rockii were extensively investigated. Both extracts and some of their compounds have the ability to scavenge free-radicals and to inhibit Candida albicans growth.

Authors+Show Affiliations

Department of Pharmaceutical and Biomedical Sciences, University of Salerno, Fisciano, Salerno, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22004890

Citation

Picerno, Patrizia, et al. "Screening of a Polar Extract of Paeonia Rockii: Composition and Antioxidant and Antifungal Activities." Journal of Ethnopharmacology, vol. 138, no. 3, 2011, pp. 705-12.
Picerno P, Mencherini T, Sansone F, et al. Screening of a polar extract of Paeonia rockii: composition and antioxidant and antifungal activities. J Ethnopharmacol. 2011;138(3):705-12.
Picerno, P., Mencherini, T., Sansone, F., Del Gaudio, P., Granata, I., Porta, A., & Aquino, R. P. (2011). Screening of a polar extract of Paeonia rockii: composition and antioxidant and antifungal activities. Journal of Ethnopharmacology, 138(3), 705-12. https://doi.org/10.1016/j.jep.2011.09.056
Picerno P, et al. Screening of a Polar Extract of Paeonia Rockii: Composition and Antioxidant and Antifungal Activities. J Ethnopharmacol. 2011 Dec 8;138(3):705-12. PubMed PMID: 22004890.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Screening of a polar extract of Paeonia rockii: composition and antioxidant and antifungal activities. AU - Picerno,Patrizia, AU - Mencherini,Teresa, AU - Sansone,Francesca, AU - Del Gaudio,Pasquale, AU - Granata,Ilaria, AU - Porta,Amalia, AU - Aquino,Rita P, Y1 - 2011/10/06/ PY - 2011/07/22/received PY - 2011/09/23/revised PY - 2011/09/29/accepted PY - 2011/10/19/entrez PY - 2011/10/19/pubmed PY - 2012/4/13/medline SP - 705 EP - 12 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 138 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The genus Paeonia (Paeoniaceae), is one of the most important source of crude drugs in traditional Chinese medicine and investigation on many species is large. Up to now studies on Paeonia rockii, one of the eight species recognized in the section Moutan, are very limited. AIM OF THE STUDY: This research aimed to investigate the composition of Paeonia rockii roots and to evaluate the in vitro free-radical scavenging and antifungal activities of a polar extract (PPR) and its major constituents. MATERIALS AND METHODS: PPR was obtained from defatted dried roots of Paeonia rockii using MeOH as extraction solvent. Its n-BuOH soluble portion (PPR-B) was purified by Sephadex LH-20 followed by RP-HPLC to give nineteen compounds belonging to the classes polyphenols, monoterpenes and triterpenes. Their structure were spectrally characterized (UV, 1D and 2D NMR, MS). The polyphenols content of PPR and PPR-B was examined by the Folin-Ciocalteau colorimetric assay and HPLC method. Both extracts (PPR and PPR-B) and their major constituents were tested for the free-radical scavenging activity by DPPH-test, and for the antifungal activity by three methods (micro-broth dilution method, XTT assay and Candida albicans morphological analysis). RESULTS: 5-Butylhydroxy-γ-lactone (1), and ethyl-arabinopyranoside (2) have been isolated for the first time as naturally occurring compounds and taxifolin (3) was reported for the first time in Paeonia spp. Nine polyphenols, four monoterpenes and three triterpenes were also identified. Both the extracts PPR and PPR-B had high polyphenol content, and high concentration of gallic acid derivatives and paeoniflorin, chemotaxonomic characteristic markers of the genus. PPR, gallic acid and methyl-gallate displayed high potency in scavenging free-radicals (DPPH test, EC(50) 13.3, 1.2, 1.9 μg/ml, respectively). Both the extracts and gallic acid individually showed an interesting antifungal property (MIC(50) at 24 h 25, 0.9 and 30 μg/ml, respectively) and notably, a combination of paeoniflorin/gallic acid (MIC(50)=0.5+20 μg/ml, respectively) was more active than the single compound in inhibiting Candida growth. CONCLUSION: The polar methanolic extract (PPR), its n-BuOH soluble fraction and constituents of Paeonia rockii were extensively investigated. Both extracts and some of their compounds have the ability to scavenge free-radicals and to inhibit Candida albicans growth. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22004890/Screening_of_a_polar_extract_of_Paeonia_rockii:_composition_and_antioxidant_and_antifungal_activities_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(11)00728-8 DB - PRIME DP - Unbound Medicine ER -