Tags

Type your tag names separated by a space and hit enter

Serology of coccidioidomycosis.
Clin Microbiol Rev. 1990 Jul; 3(3):247-68.CM

Abstract

Serologic tests have assisted in the diagnosis and prognosis of coccidioidomycosis for a half-century. The causative agent, Coccidioides immitis, is a dimorphic fungus existing in a hyphal form with arthroconidia in nature and in the usual culture. The arthroconidia represent the inhaled infective forms which in vivo and under special laboratory conditions form spherules which endosporulate. The culture filtrate/autolysate (coccidioidin) from the hyphal phase has provided antigens of suitable reliability for currently used serologic tests. These tests are primarily to determine the two major antibody responses: the early immunoglobulin M (IgM) response is useful in the diagnosis of acute primary coccidioidomycosis. Later, IgG is produced and usually outlasts the IgM, persisting in chronic coccidioidomycosis. The IgM is detectable by tube precipitin, a corresponding immunodiffusion, or latex particle agglutination tests. The pertinent antigen(s) is heat stable and pronase resistant and appears to be largely carbohydrate, mainly mannose with some 3-O-methyl mannose. The IgG detectable in the serum and other body fluids by complement fixation and a corresponding immuno-diffusion is useful in diagnosis, and its quantitation provides an indicator of progression of disease (increasing titer) or regression (decreasing titer). The pertinent antigen appears to be a heat-labile, pronase-sensitive protein which in an unreduced form has a molecular weight of 110,000. A third very useful serologic procedure is the exoantigen test for identification of putative cultures of C. immitis.

Authors+Show Affiliations

Department of Medical Microbiology and Immunology, School of Medicine, University of California, Davis 95616.No affiliation info available

Pub Type(s)

Journal Article
Review

Language

eng

PubMed ID

2200605

Citation

Pappagianis, D, and B L. Zimmer. "Serology of Coccidioidomycosis." Clinical Microbiology Reviews, vol. 3, no. 3, 1990, pp. 247-68.
Pappagianis D, Zimmer BL. Serology of coccidioidomycosis. Clin Microbiol Rev. 1990;3(3):247-68.
Pappagianis, D., & Zimmer, B. L. (1990). Serology of coccidioidomycosis. Clinical Microbiology Reviews, 3(3), 247-68.
Pappagianis D, Zimmer BL. Serology of Coccidioidomycosis. Clin Microbiol Rev. 1990;3(3):247-68. PubMed PMID: 2200605.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Serology of coccidioidomycosis. AU - Pappagianis,D, AU - Zimmer,B L, PY - 1990/7/1/pubmed PY - 1990/7/1/medline PY - 1990/7/1/entrez SP - 247 EP - 68 JF - Clinical microbiology reviews JO - Clin Microbiol Rev VL - 3 IS - 3 N2 - Serologic tests have assisted in the diagnosis and prognosis of coccidioidomycosis for a half-century. The causative agent, Coccidioides immitis, is a dimorphic fungus existing in a hyphal form with arthroconidia in nature and in the usual culture. The arthroconidia represent the inhaled infective forms which in vivo and under special laboratory conditions form spherules which endosporulate. The culture filtrate/autolysate (coccidioidin) from the hyphal phase has provided antigens of suitable reliability for currently used serologic tests. These tests are primarily to determine the two major antibody responses: the early immunoglobulin M (IgM) response is useful in the diagnosis of acute primary coccidioidomycosis. Later, IgG is produced and usually outlasts the IgM, persisting in chronic coccidioidomycosis. The IgM is detectable by tube precipitin, a corresponding immunodiffusion, or latex particle agglutination tests. The pertinent antigen(s) is heat stable and pronase resistant and appears to be largely carbohydrate, mainly mannose with some 3-O-methyl mannose. The IgG detectable in the serum and other body fluids by complement fixation and a corresponding immuno-diffusion is useful in diagnosis, and its quantitation provides an indicator of progression of disease (increasing titer) or regression (decreasing titer). The pertinent antigen appears to be a heat-labile, pronase-sensitive protein which in an unreduced form has a molecular weight of 110,000. A third very useful serologic procedure is the exoantigen test for identification of putative cultures of C. immitis. SN - 0893-8512 UR - https://www.unboundmedicine.com/medline/citation/2200605/Serology_of_coccidioidomycosis_ L2 - https://journals.asm.org/doi/10.1128/CMR.3.3.247?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -