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Detailed LC-MS/MS analysis of ciguatoxins revealing distinct regional and species characteristics in fish and causative alga from the Pacific.
Anal Chem. 2011 Dec 01; 83(23):8886-91.AC

Abstract

Toxin profiles of representative ciguatera species caught at different locations of Japan were investigated in fish flesh by high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis. Identification and quantification of 16 toxins were facilitated by the use of 14 reference toxins prepared by either synthesis or isolation from natural sources and the previous LC-MS data thereof. Sodium adduct ions [M + Na](+) were used as parent and product ions. Distinct regional differences were unveiled: ciguatoxin-1B type toxins were found in snappers and groupers from Okinawa, ciguatoxin-3C type toxins were found in a spotted knifejaw, Oplegnathus punctatus, from Miyazaki located 730 km north of Okinawa, and both types of toxins were found in a red snapper, Lutjanus bohar, from Minamitorishima (Marcus) Island. Twelve toxins were identified in a dinoflagellate, Gambierdiscus toxicus, collected as the primary toxin source in French Polynesia. Occurrence of M-seco-toxins in fish and oxidized toxins in the dinoflagellate was confirmed for the first time. The present LC-MS/MS method is rapid, specific, and accurate. It not only outperforms the currently employed mouse bioassays but also enables the study of the toxin dynamics during the food chain transmission.

Authors+Show Affiliations

Okinawa Science and Technology Promotion Center, 12-75 Suzaki, Uruma, Okinawa 904-2234, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22010820

Citation

Yogi, Kentaro, et al. "Detailed LC-MS/MS Analysis of Ciguatoxins Revealing Distinct Regional and Species Characteristics in Fish and Causative Alga From the Pacific." Analytical Chemistry, vol. 83, no. 23, 2011, pp. 8886-91.
Yogi K, Oshiro N, Inafuku Y, et al. Detailed LC-MS/MS analysis of ciguatoxins revealing distinct regional and species characteristics in fish and causative alga from the Pacific. Anal Chem. 2011;83(23):8886-91.
Yogi, K., Oshiro, N., Inafuku, Y., Hirama, M., & Yasumoto, T. (2011). Detailed LC-MS/MS analysis of ciguatoxins revealing distinct regional and species characteristics in fish and causative alga from the Pacific. Analytical Chemistry, 83(23), 8886-91. https://doi.org/10.1021/ac200799j
Yogi K, et al. Detailed LC-MS/MS Analysis of Ciguatoxins Revealing Distinct Regional and Species Characteristics in Fish and Causative Alga From the Pacific. Anal Chem. 2011 Dec 1;83(23):8886-91. PubMed PMID: 22010820.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Detailed LC-MS/MS analysis of ciguatoxins revealing distinct regional and species characteristics in fish and causative alga from the Pacific. AU - Yogi,Kentaro, AU - Oshiro,Naomasa, AU - Inafuku,Yasuo, AU - Hirama,Masahiro, AU - Yasumoto,Takeshi, Y1 - 2011/11/02/ PY - 2011/10/21/entrez PY - 2011/10/21/pubmed PY - 2012/3/22/medline SP - 8886 EP - 91 JF - Analytical chemistry JO - Anal Chem VL - 83 IS - 23 N2 - Toxin profiles of representative ciguatera species caught at different locations of Japan were investigated in fish flesh by high-performance liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis. Identification and quantification of 16 toxins were facilitated by the use of 14 reference toxins prepared by either synthesis or isolation from natural sources and the previous LC-MS data thereof. Sodium adduct ions [M + Na](+) were used as parent and product ions. Distinct regional differences were unveiled: ciguatoxin-1B type toxins were found in snappers and groupers from Okinawa, ciguatoxin-3C type toxins were found in a spotted knifejaw, Oplegnathus punctatus, from Miyazaki located 730 km north of Okinawa, and both types of toxins were found in a red snapper, Lutjanus bohar, from Minamitorishima (Marcus) Island. Twelve toxins were identified in a dinoflagellate, Gambierdiscus toxicus, collected as the primary toxin source in French Polynesia. Occurrence of M-seco-toxins in fish and oxidized toxins in the dinoflagellate was confirmed for the first time. The present LC-MS/MS method is rapid, specific, and accurate. It not only outperforms the currently employed mouse bioassays but also enables the study of the toxin dynamics during the food chain transmission. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/22010820/Detailed_LC_MS/MS_analysis_of_ciguatoxins_revealing_distinct_regional_and_species_characteristics_in_fish_and_causative_alga_from_the_Pacific_ L2 - https://doi.org/10.1021/ac200799j DB - PRIME DP - Unbound Medicine ER -