Tags

Type your tag names separated by a space and hit enter

Clinical characteristics of infections caused by Tsukamurella spp. and antimicrobial susceptibilities of the isolates.
Int J Antimicrob Agents. 2011 Dec; 38(6):534-7.IJ

Abstract

To investigate the clinical and microbiological characteristics of infections caused by Tsukamurella spp., the computerised database of the Bacteriology Laboratory at National Taiwan University Hospital (Taipei, Taiwan) was reviewed retrospectively to identify patients with infections caused by this species during the period January 1997 to December 2008. All of the isolates had been initially misidentified as Rhodococcus spp. Identification of Tsukamurella isolates to species level was carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the heat shock protein gene (hsp65) as well as 16S rRNA gene sequencing. During the study period, a total of eight patients with Tsukamurella infection and two patients with Tsukamurella colonisation were identified. Tsukamurella tyrosinosolvens (n=6) was the most prevalent species, followed by Tsukamurella spumae (n=3) and Tsukamurella pulmonis (n=1). Keratitis was the most common type of infection (n=3), followed by catheter-related bloodstream infection (n=2). One of the patients with Tsukamurella infection died due to bacteraemia; the other seven patients with Tsukamurella infection had favourable outcomes. The three species had different drug susceptibility patterns; T. pulmonis was the most resistant pathogen, with higher minimum inhibitory concentrations of clindamycin (>2 mg/L), erythromycin (2 mg/L) and tetracycline (8 mg/L) than those for the other Tsukamurella spp. In conclusion, strains of Tsukamurella spp., including T. spumae, are uncommon causative agents of ocular infections and bacteraemia in cancer patients. Molecular diagnostic methods are essential to distinguish species in the Tsukamurella genus from species in other phylogenetically related genera such as Rhodococcus.

Authors+Show Affiliations

Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22014886

Citation

Liu, Chia-Ying, et al. "Clinical Characteristics of Infections Caused By Tsukamurella Spp. and Antimicrobial Susceptibilities of the Isolates." International Journal of Antimicrobial Agents, vol. 38, no. 6, 2011, pp. 534-7.
Liu CY, Lai CC, Lee MR, et al. Clinical characteristics of infections caused by Tsukamurella spp. and antimicrobial susceptibilities of the isolates. Int J Antimicrob Agents. 2011;38(6):534-7.
Liu, C. Y., Lai, C. C., Lee, M. R., Lee, Y. C., Huang, Y. T., Liao, C. H., & Hsueh, P. R. (2011). Clinical characteristics of infections caused by Tsukamurella spp. and antimicrobial susceptibilities of the isolates. International Journal of Antimicrobial Agents, 38(6), 534-7. https://doi.org/10.1016/j.ijantimicag.2011.07.018
Liu CY, et al. Clinical Characteristics of Infections Caused By Tsukamurella Spp. and Antimicrobial Susceptibilities of the Isolates. Int J Antimicrob Agents. 2011;38(6):534-7. PubMed PMID: 22014886.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Clinical characteristics of infections caused by Tsukamurella spp. and antimicrobial susceptibilities of the isolates. AU - Liu,Chia-Ying, AU - Lai,Chih-Cheng, AU - Lee,Meng-Rui, AU - Lee,Yi-Chieh, AU - Huang,Yu-Tsung, AU - Liao,Chun-Hsing, AU - Hsueh,Po-Ren, Y1 - 2011/10/19/ PY - 2011/06/12/received PY - 2011/07/29/revised PY - 2011/07/29/accepted PY - 2011/10/22/entrez PY - 2011/10/22/pubmed PY - 2012/3/2/medline SP - 534 EP - 7 JF - International journal of antimicrobial agents JO - Int. J. Antimicrob. Agents VL - 38 IS - 6 N2 - To investigate the clinical and microbiological characteristics of infections caused by Tsukamurella spp., the computerised database of the Bacteriology Laboratory at National Taiwan University Hospital (Taipei, Taiwan) was reviewed retrospectively to identify patients with infections caused by this species during the period January 1997 to December 2008. All of the isolates had been initially misidentified as Rhodococcus spp. Identification of Tsukamurella isolates to species level was carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the heat shock protein gene (hsp65) as well as 16S rRNA gene sequencing. During the study period, a total of eight patients with Tsukamurella infection and two patients with Tsukamurella colonisation were identified. Tsukamurella tyrosinosolvens (n=6) was the most prevalent species, followed by Tsukamurella spumae (n=3) and Tsukamurella pulmonis (n=1). Keratitis was the most common type of infection (n=3), followed by catheter-related bloodstream infection (n=2). One of the patients with Tsukamurella infection died due to bacteraemia; the other seven patients with Tsukamurella infection had favourable outcomes. The three species had different drug susceptibility patterns; T. pulmonis was the most resistant pathogen, with higher minimum inhibitory concentrations of clindamycin (>2 mg/L), erythromycin (2 mg/L) and tetracycline (8 mg/L) than those for the other Tsukamurella spp. In conclusion, strains of Tsukamurella spp., including T. spumae, are uncommon causative agents of ocular infections and bacteraemia in cancer patients. Molecular diagnostic methods are essential to distinguish species in the Tsukamurella genus from species in other phylogenetically related genera such as Rhodococcus. SN - 1872-7913 UR - https://www.unboundmedicine.com/medline/citation/22014886/Clinical_characteristics_of_infections_caused_by_Tsukamurella_spp__and_antimicrobial_susceptibilities_of_the_isolates_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(11)00354-2 DB - PRIME DP - Unbound Medicine ER -