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SecE-dependent overproduction of SecY in Escherichia coli. Evidence for interaction between two components of the secretory machinery.
FEBS Lett 1990; 269(1):96-100FL

Abstract

The secY and secE genes were individually cloned and placed under the control of the tac promoter on plasmids. Induction with isopropyl-beta-D-thiogalactopyranoside resulted in the overproduction of SecE, but not that of SecY. The simultaneous induced expression of both genes in the same cells resulted in the overproduction of SecY together with that of SecE. SecY and SecE thus overproduced were localized in the cytoplasmic membrane as those expressed at the normal levels were. It is suggested that SecY and SecE interact with each other in the cytoplasmic membrane. The numbers of the SecY and SecE molecules per cell were estimated.

Authors+Show Affiliations

Institute of Applied Microbiology, University of Tokyo, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2201574

Citation

Matsuyama, S, et al. "SecE-dependent Overproduction of SecY in Escherichia Coli. Evidence for Interaction Between Two Components of the Secretory Machinery." FEBS Letters, vol. 269, no. 1, 1990, pp. 96-100.
Matsuyama S, Akimaru J, Mizushima S. SecE-dependent overproduction of SecY in Escherichia coli. Evidence for interaction between two components of the secretory machinery. FEBS Lett. 1990;269(1):96-100.
Matsuyama, S., Akimaru, J., & Mizushima, S. (1990). SecE-dependent overproduction of SecY in Escherichia coli. Evidence for interaction between two components of the secretory machinery. FEBS Letters, 269(1), pp. 96-100.
Matsuyama S, Akimaru J, Mizushima S. SecE-dependent Overproduction of SecY in Escherichia Coli. Evidence for Interaction Between Two Components of the Secretory Machinery. FEBS Lett. 1990 Aug 20;269(1):96-100. PubMed PMID: 2201574.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - SecE-dependent overproduction of SecY in Escherichia coli. Evidence for interaction between two components of the secretory machinery. AU - Matsuyama,S, AU - Akimaru,J, AU - Mizushima,S, PY - 1990/8/20/pubmed PY - 1990/8/20/medline PY - 1990/8/20/entrez SP - 96 EP - 100 JF - FEBS letters JO - FEBS Lett. VL - 269 IS - 1 N2 - The secY and secE genes were individually cloned and placed under the control of the tac promoter on plasmids. Induction with isopropyl-beta-D-thiogalactopyranoside resulted in the overproduction of SecE, but not that of SecY. The simultaneous induced expression of both genes in the same cells resulted in the overproduction of SecY together with that of SecE. SecY and SecE thus overproduced were localized in the cytoplasmic membrane as those expressed at the normal levels were. It is suggested that SecY and SecE interact with each other in the cytoplasmic membrane. The numbers of the SecY and SecE molecules per cell were estimated. SN - 0014-5793 UR - https://www.unboundmedicine.com/medline/citation/2201574/SecE_dependent_overproduction_of_SecY_in_Escherichia_coli__Evidence_for_interaction_between_two_components_of_the_secretory_machinery_ L2 - https://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0014-5793&date=1990&volume=269&issue=1&spage=96 DB - PRIME DP - Unbound Medicine ER -