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Characterization by high-performance liquid chromatography with diode-array detection coupled to time-of-flight mass spectrometry of the phenolic fraction in a cranberry syrup used to prevent urinary tract diseases, together with a study of its antibacterial activity.
J Pharm Biomed Anal 2012; 58:34-41JP

Abstract

The phenolic fraction of a commercial cranberry syrup, which is purported to have good properties for the prevention of urinary diseases, has been thoroughly characterized using HPLC-DAD-TOF-MS. A study of its antibacterial activity has also been carried out. For this purpose a new HPLC-DAD-TOF-MS method using negative and positive ionization modes was developed and it was thus possible to identify 34 different compounds, nine of which have been tentatively characterized for the first time in cranberry syrup. It is also important to highlight that different coumarins in this matrix were also determined, which, to our knowledge, have not been found previously in the cranberry. The phenolic fraction obtained by HPLC-DAD was found to be 5.47 mg/mL. Catechin and procyanidins belonging to flavanols were the family of compounds found at the highest concentrations (2.37 mg/mL); flavonols were at a concentration of 1.91 mg/mL and phenolic-acid derivatives were found at the lowest concentration (0.15 mg/mL). With regard to antibacterial activity, the incubation of Escherichia coli with cranberry syrup was found to reduce surface hydrophobicity as a function of the concentration of the extract.

Authors+Show Affiliations

Department of Analytical Chemistry, Faculty of Sciences, University of Granada, Avenida Fuentenueva s/n, 18071 Granada, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22018892

Citation

Iswaldi, Ihsan, et al. "Characterization By High-performance Liquid Chromatography With Diode-array Detection Coupled to Time-of-flight Mass Spectrometry of the Phenolic Fraction in a Cranberry Syrup Used to Prevent Urinary Tract Diseases, Together With a Study of Its Antibacterial Activity." Journal of Pharmaceutical and Biomedical Analysis, vol. 58, 2012, pp. 34-41.
Iswaldi I, Gómez-Caravaca AM, Arráez-Román D, et al. Characterization by high-performance liquid chromatography with diode-array detection coupled to time-of-flight mass spectrometry of the phenolic fraction in a cranberry syrup used to prevent urinary tract diseases, together with a study of its antibacterial activity. J Pharm Biomed Anal. 2012;58:34-41.
Iswaldi, I., Gómez-Caravaca, A. M., Arráez-Román, D., Uberos, J., Lardón, M., Segura-Carretero, A., & Fernández-Gutiérrez, A. (2012). Characterization by high-performance liquid chromatography with diode-array detection coupled to time-of-flight mass spectrometry of the phenolic fraction in a cranberry syrup used to prevent urinary tract diseases, together with a study of its antibacterial activity. Journal of Pharmaceutical and Biomedical Analysis, 58, pp. 34-41. doi:10.1016/j.jpba.2011.09.027.
Iswaldi I, et al. Characterization By High-performance Liquid Chromatography With Diode-array Detection Coupled to Time-of-flight Mass Spectrometry of the Phenolic Fraction in a Cranberry Syrup Used to Prevent Urinary Tract Diseases, Together With a Study of Its Antibacterial Activity. J Pharm Biomed Anal. 2012 Jan 25;58:34-41. PubMed PMID: 22018892.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization by high-performance liquid chromatography with diode-array detection coupled to time-of-flight mass spectrometry of the phenolic fraction in a cranberry syrup used to prevent urinary tract diseases, together with a study of its antibacterial activity. AU - Iswaldi,Ihsan, AU - Gómez-Caravaca,Ana María, AU - Arráez-Román,David, AU - Uberos,José, AU - Lardón,Marita, AU - Segura-Carretero,Antonio, AU - Fernández-Gutiérrez,Alberto, Y1 - 2011/10/01/ PY - 2011/03/23/received PY - 2011/09/21/revised PY - 2011/09/24/accepted PY - 2011/10/25/entrez PY - 2011/10/25/pubmed PY - 2012/5/23/medline SP - 34 EP - 41 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 58 N2 - The phenolic fraction of a commercial cranberry syrup, which is purported to have good properties for the prevention of urinary diseases, has been thoroughly characterized using HPLC-DAD-TOF-MS. A study of its antibacterial activity has also been carried out. For this purpose a new HPLC-DAD-TOF-MS method using negative and positive ionization modes was developed and it was thus possible to identify 34 different compounds, nine of which have been tentatively characterized for the first time in cranberry syrup. It is also important to highlight that different coumarins in this matrix were also determined, which, to our knowledge, have not been found previously in the cranberry. The phenolic fraction obtained by HPLC-DAD was found to be 5.47 mg/mL. Catechin and procyanidins belonging to flavanols were the family of compounds found at the highest concentrations (2.37 mg/mL); flavonols were at a concentration of 1.91 mg/mL and phenolic-acid derivatives were found at the lowest concentration (0.15 mg/mL). With regard to antibacterial activity, the incubation of Escherichia coli with cranberry syrup was found to reduce surface hydrophobicity as a function of the concentration of the extract. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/22018892/Characterization_by_high_performance_liquid_chromatography_with_diode_array_detection_coupled_to_time_of_flight_mass_spectrometry_of_the_phenolic_fraction_in_a_cranberry_syrup_used_to_prevent_urinary_tract_diseases_together_with_a_study_of_its_antibacterial_activity_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(11)00545-0 DB - PRIME DP - Unbound Medicine ER -