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Loop-mediated isothermal amplification: rapid detection of Angiostrongylus cantonensis infection in Pomacea canaliculata.
Parasit Vectors. 2011 Oct 25; 4:204.PV

Abstract

BACKGROUND

Angiostrongylus cantonensis is a zoonotic parasite that causes eosinophilic meningitis in humans. The most common source of infection with A. cantonensis is the consumption of raw or undercooked mollusks (e.g., snails and slugs) harbouring infectious third-stage larvae (L3). However, the parasite is difficult to identify in snails. The purpose of this study was to develop a quick, simple molecular method to survey for A. cantonensis in intermediate host snails.

FINDINGS

We used a loop-mediated isothermal amplification (LAMP) assay, which was performed using Bst DNA polymerase. Reactions amplified the A. cantonensis 18S rRNA gene and demonstrated high sensitivity; as little as 1 fg of DNA was detected in the samples. Furthermore, no cross-reactivity was found with other parasites such as Toxoplasma gondii, Plasmodium falciparum, Schistosoma japonicum, Clonorchis sinensis, Paragonimus westermani and Anisakis. Pomacea canaliculata snails were exposed to A. cantonensis first-stage larvae (L1) in the laboratory, and L3 were observed in the snails thirty-five days after infection. All nine samples were positive as determined by the LAMP assay for A. cantonensis, which was identified as positive by using PCR and microscopy, this demonstrates that LAMP is sensitive and effective for diagnosis.

CONCLUSIONS

LAMP is an appropriate diagnostic method for the routine identification of A. cantonensis within its intermediate host snail P. canaliculata because of its simplicity, sensitivity, and specificity. It holds great promise as a useful monitoring tool for A. cantonensis in endemic regions.

Authors+Show Affiliations

Institute of Parasitology, Zhejiang Academy of Medical Sciences, Hangzhou, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22023992

Citation

Chen, Rui, et al. "Loop-mediated Isothermal Amplification: Rapid Detection of Angiostrongylus Cantonensis Infection in Pomacea Canaliculata." Parasites & Vectors, vol. 4, 2011, p. 204.
Chen R, Tong Q, Zhang Y, et al. Loop-mediated isothermal amplification: rapid detection of Angiostrongylus cantonensis infection in Pomacea canaliculata. Parasit Vectors. 2011;4:204.
Chen, R., Tong, Q., Zhang, Y., Lou, D., Kong, Q., Lv, S., Zhuo, M., Wen, L., & Lu, S. (2011). Loop-mediated isothermal amplification: rapid detection of Angiostrongylus cantonensis infection in Pomacea canaliculata. Parasites & Vectors, 4, 204. https://doi.org/10.1186/1756-3305-4-204
Chen R, et al. Loop-mediated Isothermal Amplification: Rapid Detection of Angiostrongylus Cantonensis Infection in Pomacea Canaliculata. Parasit Vectors. 2011 Oct 25;4:204. PubMed PMID: 22023992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Loop-mediated isothermal amplification: rapid detection of Angiostrongylus cantonensis infection in Pomacea canaliculata. AU - Chen,Rui, AU - Tong,QunBo, AU - Zhang,Yi, AU - Lou,Di, AU - Kong,QingMing, AU - Lv,Shan, AU - Zhuo,MingMing, AU - Wen,LiYong, AU - Lu,ShaoHong, Y1 - 2011/10/25/ PY - 2011/03/23/received PY - 2011/10/25/accepted PY - 2011/10/26/entrez PY - 2011/10/26/pubmed PY - 2012/2/9/medline SP - 204 EP - 204 JF - Parasites & vectors JO - Parasit Vectors VL - 4 N2 - BACKGROUND: Angiostrongylus cantonensis is a zoonotic parasite that causes eosinophilic meningitis in humans. The most common source of infection with A. cantonensis is the consumption of raw or undercooked mollusks (e.g., snails and slugs) harbouring infectious third-stage larvae (L3). However, the parasite is difficult to identify in snails. The purpose of this study was to develop a quick, simple molecular method to survey for A. cantonensis in intermediate host snails. FINDINGS: We used a loop-mediated isothermal amplification (LAMP) assay, which was performed using Bst DNA polymerase. Reactions amplified the A. cantonensis 18S rRNA gene and demonstrated high sensitivity; as little as 1 fg of DNA was detected in the samples. Furthermore, no cross-reactivity was found with other parasites such as Toxoplasma gondii, Plasmodium falciparum, Schistosoma japonicum, Clonorchis sinensis, Paragonimus westermani and Anisakis. Pomacea canaliculata snails were exposed to A. cantonensis first-stage larvae (L1) in the laboratory, and L3 were observed in the snails thirty-five days after infection. All nine samples were positive as determined by the LAMP assay for A. cantonensis, which was identified as positive by using PCR and microscopy, this demonstrates that LAMP is sensitive and effective for diagnosis. CONCLUSIONS: LAMP is an appropriate diagnostic method for the routine identification of A. cantonensis within its intermediate host snail P. canaliculata because of its simplicity, sensitivity, and specificity. It holds great promise as a useful monitoring tool for A. cantonensis in endemic regions. SN - 1756-3305 UR - https://www.unboundmedicine.com/medline/citation/22023992/Loop_mediated_isothermal_amplification:_rapid_detection_of_Angiostrongylus_cantonensis_infection_in_Pomacea_canaliculata_ L2 - https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-4-204 DB - PRIME DP - Unbound Medicine ER -