Tags

Type your tag names separated by a space and hit enter

Nonsense-mediated mRNA decay factors, UPF1 and UPF3, contribute to plant defense.
Plant Cell Physiol. 2011 Dec; 52(12):2147-56.PC

Abstract

In Arabidopsis, the NMD-defective mutants upf1-5 and upf3-1 are characterized by dwarfism, curly leaves and late flowering. These phenotypes are similar to those of mutants showing constitutive pathogenesis-related (PR) gene expression, salicylic acid (SA) accumulation and, subsequently, resistance to pathogens. The disease symptoms of upf1-5 and upf3-1 mutants were observed following infection with the virulent pathogen Pst DC3000 with the aim of determining whether the loss of nonsense-mediated mRNA decay (NMD) is involved in disease resistance. These mutant plants showed not only enhanced resistance to Pst DC3000, but also elevated levels of endogenous SA, PR gene transcripts and WRKY transcripts. UPF1 and UPF3 expression was down-regulated in Pst DC3000-infected Arabidopsis plants, but the expression of various NMD target genes was up-regulated. The expression of 10 defense-related genes was elevated in cycloheximide (CHX)-treated plants. The transcriptional ratios of eight of these 10 defense-related genes in CHX-treated to non-treated plants were lower in NMD-defective mutants than in the wild-type plants. These eight defense-related genes are possibly regulated by the NMD mechanism, and it is clear that an alternatively spliced transcript of WRKY62, which contains a premature termination codon, was regulated by this mechanism. Taken together, our results suggest that UPF1 and UPF3, which are key NMD factors, may act as defense-related regulators associated with plant immunity.

Authors+Show Affiliations

School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22025558

Citation

Jeong, Hee-Jeong, et al. "Nonsense-mediated mRNA Decay Factors, UPF1 and UPF3, Contribute to Plant Defense." Plant & Cell Physiology, vol. 52, no. 12, 2011, pp. 2147-56.
Jeong HJ, Kim YJ, Kim SH, et al. Nonsense-mediated mRNA decay factors, UPF1 and UPF3, contribute to plant defense. Plant Cell Physiol. 2011;52(12):2147-56.
Jeong, H. J., Kim, Y. J., Kim, S. H., Kim, Y. H., Lee, I. J., Kim, Y. K., & Shin, J. S. (2011). Nonsense-mediated mRNA decay factors, UPF1 and UPF3, contribute to plant defense. Plant & Cell Physiology, 52(12), 2147-56. https://doi.org/10.1093/pcp/pcr144
Jeong HJ, et al. Nonsense-mediated mRNA Decay Factors, UPF1 and UPF3, Contribute to Plant Defense. Plant Cell Physiol. 2011;52(12):2147-56. PubMed PMID: 22025558.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Nonsense-mediated mRNA decay factors, UPF1 and UPF3, contribute to plant defense. AU - Jeong,Hee-Jeong, AU - Kim,Young Jin, AU - Kim,Sang Hyon, AU - Kim,Yoon-Ha, AU - Lee,In-Jung, AU - Kim,Yoon Ki, AU - Shin,Jeong Sheop, Y1 - 2011/10/24/ PY - 2011/10/26/entrez PY - 2011/10/26/pubmed PY - 2012/4/11/medline SP - 2147 EP - 56 JF - Plant & cell physiology JO - Plant Cell Physiol. VL - 52 IS - 12 N2 - In Arabidopsis, the NMD-defective mutants upf1-5 and upf3-1 are characterized by dwarfism, curly leaves and late flowering. These phenotypes are similar to those of mutants showing constitutive pathogenesis-related (PR) gene expression, salicylic acid (SA) accumulation and, subsequently, resistance to pathogens. The disease symptoms of upf1-5 and upf3-1 mutants were observed following infection with the virulent pathogen Pst DC3000 with the aim of determining whether the loss of nonsense-mediated mRNA decay (NMD) is involved in disease resistance. These mutant plants showed not only enhanced resistance to Pst DC3000, but also elevated levels of endogenous SA, PR gene transcripts and WRKY transcripts. UPF1 and UPF3 expression was down-regulated in Pst DC3000-infected Arabidopsis plants, but the expression of various NMD target genes was up-regulated. The expression of 10 defense-related genes was elevated in cycloheximide (CHX)-treated plants. The transcriptional ratios of eight of these 10 defense-related genes in CHX-treated to non-treated plants were lower in NMD-defective mutants than in the wild-type plants. These eight defense-related genes are possibly regulated by the NMD mechanism, and it is clear that an alternatively spliced transcript of WRKY62, which contains a premature termination codon, was regulated by this mechanism. Taken together, our results suggest that UPF1 and UPF3, which are key NMD factors, may act as defense-related regulators associated with plant immunity. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/22025558/Nonsense_mediated_mRNA_decay_factors_UPF1_and_UPF3_contribute_to_plant_defense_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcr144 DB - PRIME DP - Unbound Medicine ER -