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Hair nicotine/cotinine concentrations as a method of monitoring exposure to tobacco smoke among infants and adults.
Hum Exp Toxicol. 2012 Mar; 31(3):258-65.HE

Abstract

In this pilot study, we examined the validity and usefulness of hair nicotine-cotinine evaluation as a biomarker of monitoring exposure to tobacco. Head hair samples were collected from 22 infants (<2 years of age) and 44 adults with different exposures to tobacco (through either active or passive smoking) and analyzed by liquid chromatography-mass spectrometry (LC-MS) for nicotine and cotinine. Hair samples were divided into three groups, infants, passive smoker adults and active smoker adults, and into eight subgroups according to the degree of exposure. The limit of quantification (LOQ) was 0.1 ng/mg for nicotine and 0.05 ng/mg for cotinine. Mean recovery was 69.15% for nicotine and 72.08% for cotinine. The within- and between-day precision for cotinine and nicotine was calculated at different concentrations. Moreover, hair nicotine and cotinine concentrations were highly correlated among adult active smokers (R (2) = 0.710, p < 0.001), among adult nonsmokers exposed to secondhand smoke (SHS; R (2) = 0.729, p < 0.001) and among infants (R (2) = 0.538, p = 0.01). Among the infants exposed to SHS from both parents the noted correlations were even stronger (R (2) = 0.835, p = 0.02). The above results identify the use of hair samples as an effective method for assessing exposure to tobacco, with a high association between nicotine and cotinine especially among infants heavily exposed to SHS.

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Authors+Show Affiliations

Tzatzarakis MN
Centre of Toxicology Sciences and Research, Division of Morphology, Medical School, University of Crete, Heraklion, Crete, Greece.
Vardavas CI
No affiliation info available
Terzi I
No affiliation info available
Kavalakis M
No affiliation info available
Kokkinakis M
No affiliation info available
Liesivuori J
No affiliation info available
Tsatsakis AM
No affiliation info available

MeSH

AdultAir PollutantsBiomarkersChromatography, High Pressure LiquidCotinineEnvironmental ExposureEnvironmental MonitoringFemaleHairHumansInfantInhalation ExposureLimit of DetectionMaleNicotineReproducibility of ResultsSpectrometry, Mass, Electrospray IonizationTobacco Smoke Pollution

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22027507

Citation

Tzatzarakis, M N., et al. "Hair Nicotine/cotinine Concentrations as a Method of Monitoring Exposure to Tobacco Smoke Among Infants and Adults." Human & Experimental Toxicology, vol. 31, no. 3, 2012, pp. 258-65.
Tzatzarakis MN, Vardavas CI, Terzi I, et al. Hair nicotine/cotinine concentrations as a method of monitoring exposure to tobacco smoke among infants and adults. Hum Exp Toxicol. 2012;31(3):258-65.
Tzatzarakis, M. N., Vardavas, C. I., Terzi, I., Kavalakis, M., Kokkinakis, M., Liesivuori, J., & Tsatsakis, A. M. (2012). Hair nicotine/cotinine concentrations as a method of monitoring exposure to tobacco smoke among infants and adults. Human & Experimental Toxicology, 31(3), 258-65. https://doi.org/10.1177/0960327111422401
Tzatzarakis MN, et al. Hair Nicotine/cotinine Concentrations as a Method of Monitoring Exposure to Tobacco Smoke Among Infants and Adults. Hum Exp Toxicol. 2012;31(3):258-65. PubMed PMID: 22027507.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hair nicotine/cotinine concentrations as a method of monitoring exposure to tobacco smoke among infants and adults. AU - Tzatzarakis,M N, AU - Vardavas,C I, AU - Terzi,I, AU - Kavalakis,M, AU - Kokkinakis,M, AU - Liesivuori,J, AU - Tsatsakis,A M, Y1 - 2011/10/25/ PY - 2011/10/27/entrez PY - 2011/10/27/pubmed PY - 2012/9/5/medline SP - 258 EP - 65 JF - Human & experimental toxicology JO - Hum Exp Toxicol VL - 31 IS - 3 N2 - In this pilot study, we examined the validity and usefulness of hair nicotine-cotinine evaluation as a biomarker of monitoring exposure to tobacco. Head hair samples were collected from 22 infants (<2 years of age) and 44 adults with different exposures to tobacco (through either active or passive smoking) and analyzed by liquid chromatography-mass spectrometry (LC-MS) for nicotine and cotinine. Hair samples were divided into three groups, infants, passive smoker adults and active smoker adults, and into eight subgroups according to the degree of exposure. The limit of quantification (LOQ) was 0.1 ng/mg for nicotine and 0.05 ng/mg for cotinine. Mean recovery was 69.15% for nicotine and 72.08% for cotinine. The within- and between-day precision for cotinine and nicotine was calculated at different concentrations. Moreover, hair nicotine and cotinine concentrations were highly correlated among adult active smokers (R (2) = 0.710, p < 0.001), among adult nonsmokers exposed to secondhand smoke (SHS; R (2) = 0.729, p < 0.001) and among infants (R (2) = 0.538, p = 0.01). Among the infants exposed to SHS from both parents the noted correlations were even stronger (R (2) = 0.835, p = 0.02). The above results identify the use of hair samples as an effective method for assessing exposure to tobacco, with a high association between nicotine and cotinine especially among infants heavily exposed to SHS. SN - 1477-0903 UR - https://www.unboundmedicine.com/medline/citation/22027507/Hair_nicotine/cotinine_concentrations_as_a_method_of_monitoring_exposure_to_tobacco_smoke_among_infants_and_adults_ L2 - https://journals.sagepub.com/doi/10.1177/0960327111422401?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -