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Surface modification of an acoustic biosensor allowing the detection of low concentrations of cancer markers.
Anal Biochem. 2012 Jan 15; 420(2):188-90.AB

Abstract

Analyte detection with biosensors is strongly influenced by the preparation of the biosensor surface including choice of sensing layers and coupling methods for corresponding capture molecules. We investigated the influence of different coupling procedures, especially considering coupling chemistry and incubation times for reagents, by means of surface acoustic wave (SAW) biosensors. The effect on the signal response was tested in two subsequent protein assays. Our optimized coupling procedure allowed the detection of the breast cancer markers HER-2 (human epidermal growth factor receptor-2) and TIMP-1 (tissue inhibitor of metalloproteinase-1) below the respective clinical cutoff values of only a few nanograms per milliliter.

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Authors+Show Affiliations

Gruhl FJ
Karlsruhe Institute of Technology (KIT), Institute for Microstructure Technology, 76344 Eggenstein-Leopoldshafen, Germany. friederike.gruhl@kit.edu
Länge K
No affiliation info available

MeSH

AcousticsAntibodies, ImmobilizedBiomarkers, TumorBiosensing TechniquesBiotinylationHumansLimit of DetectionReceptor, ErbB-2Staphylococcal Protein ATissue Inhibitor of Metalloproteinase-1

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22033293

Citation

Gruhl, Friederike J., and Kerstin Länge. "Surface Modification of an Acoustic Biosensor Allowing the Detection of Low Concentrations of Cancer Markers." Analytical Biochemistry, vol. 420, no. 2, 2012, pp. 188-90.
Gruhl FJ, Länge K. Surface modification of an acoustic biosensor allowing the detection of low concentrations of cancer markers. Anal Biochem. 2012;420(2):188-90.
Gruhl, F. J., & Länge, K. (2012). Surface modification of an acoustic biosensor allowing the detection of low concentrations of cancer markers. Analytical Biochemistry, 420(2), 188-90. https://doi.org/10.1016/j.ab.2011.10.006
Gruhl FJ, Länge K. Surface Modification of an Acoustic Biosensor Allowing the Detection of Low Concentrations of Cancer Markers. Anal Biochem. 2012 Jan 15;420(2):188-90. PubMed PMID: 22033293.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface modification of an acoustic biosensor allowing the detection of low concentrations of cancer markers. AU - Gruhl,Friederike J, AU - Länge,Kerstin, Y1 - 2011/10/08/ PY - 2011/06/27/received PY - 2011/09/12/revised PY - 2011/10/03/accepted PY - 2011/10/29/entrez PY - 2011/10/29/pubmed PY - 2012/3/2/medline SP - 188 EP - 90 JF - Analytical biochemistry JO - Anal Biochem VL - 420 IS - 2 N2 - Analyte detection with biosensors is strongly influenced by the preparation of the biosensor surface including choice of sensing layers and coupling methods for corresponding capture molecules. We investigated the influence of different coupling procedures, especially considering coupling chemistry and incubation times for reagents, by means of surface acoustic wave (SAW) biosensors. The effect on the signal response was tested in two subsequent protein assays. Our optimized coupling procedure allowed the detection of the breast cancer markers HER-2 (human epidermal growth factor receptor-2) and TIMP-1 (tissue inhibitor of metalloproteinase-1) below the respective clinical cutoff values of only a few nanograms per milliliter. SN - 1096-0309 UR - https://www.unboundmedicine.com/medline/citation/22033293/Surface_modification_of_an_acoustic_biosensor_allowing_the_detection_of_low_concentrations_of_cancer_markers_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(11)00658-0 DB - PRIME DP - Unbound Medicine ER -