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Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications.
Chem Biol 2011; 18(10):1241-51CB

Abstract

Advanced fluorescence imaging, including subdiffraction microscopy, relies on fluorophores with controllable emission properties. Chief among these fluorophores are the photoactivatable fluorescent proteins capable of reversible on/off photoswitching or irreversible green-to-red photoconversion. IrisFP was recently reported as the first fluorescent protein combining these two types of phototransformations. The introduction of this protein resulted in new applications such as super-resolution pulse-chase imaging. However, the spectroscopic properties of IrisFP are far from being optimal and its tetrameric organization complicates its use as a fusion tag. Here, we demonstrate how four-state optical highlighting can be rationally introduced into photoconvertible fluorescent proteins and develop and characterize a new set of such enhanced optical highlighters derived from mEosFP and Dendra2. We present in particular NijiFP, a promising new fluorescent protein with photoconvertible and biphotochromic properties that make it ideal for advanced fluorescence-based imaging applications.

Authors+Show Affiliations

Laboratory of Photochemistry and Spectroscopy, Department of Chemistry, Katholieke Universiteit Leuven, 3001 Heverlee, Belgium. virgile.adam@ibs.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22035793

Citation

Adam, Virgile, et al. "Rational Design of Photoconvertible and Biphotochromic Fluorescent Proteins for Advanced Microscopy Applications." Chemistry & Biology, vol. 18, no. 10, 2011, pp. 1241-51.
Adam V, Moeyaert B, David CC, et al. Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications. Chem Biol. 2011;18(10):1241-51.
Adam, V., Moeyaert, B., David, C. C., Mizuno, H., Lelimousin, M., Dedecker, P., ... Hofkens, J. (2011). Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications. Chemistry & Biology, 18(10), pp. 1241-51. doi:10.1016/j.chembiol.2011.08.007.
Adam V, et al. Rational Design of Photoconvertible and Biphotochromic Fluorescent Proteins for Advanced Microscopy Applications. Chem Biol. 2011 Oct 28;18(10):1241-51. PubMed PMID: 22035793.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rational design of photoconvertible and biphotochromic fluorescent proteins for advanced microscopy applications. AU - Adam,Virgile, AU - Moeyaert,Benjamien, AU - David,Charlotte C, AU - Mizuno,Hideaki, AU - Lelimousin,Mickaël, AU - Dedecker,Peter, AU - Ando,Ryoko, AU - Miyawaki,Atsushi, AU - Michiels,Jan, AU - Engelborghs,Yves, AU - Hofkens,Johan, PY - 2011/05/31/received PY - 2011/08/04/revised PY - 2011/08/08/accepted PY - 2011/11/1/entrez PY - 2011/11/1/pubmed PY - 2012/4/3/medline SP - 1241 EP - 51 JF - Chemistry & biology JO - Chem. Biol. VL - 18 IS - 10 N2 - Advanced fluorescence imaging, including subdiffraction microscopy, relies on fluorophores with controllable emission properties. Chief among these fluorophores are the photoactivatable fluorescent proteins capable of reversible on/off photoswitching or irreversible green-to-red photoconversion. IrisFP was recently reported as the first fluorescent protein combining these two types of phototransformations. The introduction of this protein resulted in new applications such as super-resolution pulse-chase imaging. However, the spectroscopic properties of IrisFP are far from being optimal and its tetrameric organization complicates its use as a fusion tag. Here, we demonstrate how four-state optical highlighting can be rationally introduced into photoconvertible fluorescent proteins and develop and characterize a new set of such enhanced optical highlighters derived from mEosFP and Dendra2. We present in particular NijiFP, a promising new fluorescent protein with photoconvertible and biphotochromic properties that make it ideal for advanced fluorescence-based imaging applications. SN - 1879-1301 UR - https://www.unboundmedicine.com/medline/citation/22035793/Rational_design_of_photoconvertible_and_biphotochromic_fluorescent_proteins_for_advanced_microscopy_applications_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1074-5521(11)00315-2 DB - PRIME DP - Unbound Medicine ER -