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Inefficient splicing of segment 7 and 8 mRNAs is an inherent property of influenza virus A/Brevig Mission/1918/1 (H1N1) that causes elevated expression of NS1 protein.
Virology. 2012 Jan 05; 422(1):46-58.V

Abstract

Influenza A virus encodes two segments (7 and 8) that produce mRNAs that can be spliced. We have investigated if naturally occurring sequence polymorphisms in the influenza A virus family affects splicing of these viral mRNAs, as that could potentially alter the NS1/NS2- and/or M1/M2-protein ratios. We compared splicing efficiency of segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) and A/Netherlands/178/95 (H3N2), as well as various H5N1 avian strains. Results revealed that both segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) were inefficiently spliced compared to other influenza virus segment 7 and 8 mRNAs. This resulted in production of higher levels of functional NS1 protein, which could potentially contribute to the pathogenic properties of the A/Brevig Mission/1918/1 (H1N1). We also show that A/Brevig Mission/1918/1 (H1N1) segment 8 mRNAs responded differently to overexpression of SR proteins than A/Netherlands/178/95 (H3N2).

Authors+Show Affiliations

Department of Laboratory Medicine, Section of Medical Microbiology, Lund University, 221 84 Lund, Sweden.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22036312

Citation

Backström Winquist, Ellenor, et al. "Inefficient Splicing of Segment 7 and 8 mRNAs Is an Inherent Property of Influenza Virus A/Brevig Mission/1918/1 (H1N1) That Causes Elevated Expression of NS1 Protein." Virology, vol. 422, no. 1, 2012, pp. 46-58.
Backström Winquist E, Abdurahman S, Tranell A, et al. Inefficient splicing of segment 7 and 8 mRNAs is an inherent property of influenza virus A/Brevig Mission/1918/1 (H1N1) that causes elevated expression of NS1 protein. Virology. 2012;422(1):46-58.
Backström Winquist, E., Abdurahman, S., Tranell, A., Lindström, S., Tingsborg, S., & Schwartz, S. (2012). Inefficient splicing of segment 7 and 8 mRNAs is an inherent property of influenza virus A/Brevig Mission/1918/1 (H1N1) that causes elevated expression of NS1 protein. Virology, 422(1), 46-58. https://doi.org/10.1016/j.virol.2011.10.004
Backström Winquist E, et al. Inefficient Splicing of Segment 7 and 8 mRNAs Is an Inherent Property of Influenza Virus A/Brevig Mission/1918/1 (H1N1) That Causes Elevated Expression of NS1 Protein. Virology. 2012 Jan 5;422(1):46-58. PubMed PMID: 22036312.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inefficient splicing of segment 7 and 8 mRNAs is an inherent property of influenza virus A/Brevig Mission/1918/1 (H1N1) that causes elevated expression of NS1 protein. AU - Backström Winquist,Ellenor, AU - Abdurahman,Samir, AU - Tranell,Anna, AU - Lindström,Sofia, AU - Tingsborg,Susanne, AU - Schwartz,Stefan, Y1 - 2011/10/28/ PY - 2011/08/28/received PY - 2011/09/16/revised PY - 2011/10/05/accepted PY - 2011/11/1/entrez PY - 2011/11/1/pubmed PY - 2012/1/21/medline SP - 46 EP - 58 JF - Virology JO - Virology VL - 422 IS - 1 N2 - Influenza A virus encodes two segments (7 and 8) that produce mRNAs that can be spliced. We have investigated if naturally occurring sequence polymorphisms in the influenza A virus family affects splicing of these viral mRNAs, as that could potentially alter the NS1/NS2- and/or M1/M2-protein ratios. We compared splicing efficiency of segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) and A/Netherlands/178/95 (H3N2), as well as various H5N1 avian strains. Results revealed that both segment 7 and 8 mRNAs of A/Brevig Mission/1918/1 (H1N1) were inefficiently spliced compared to other influenza virus segment 7 and 8 mRNAs. This resulted in production of higher levels of functional NS1 protein, which could potentially contribute to the pathogenic properties of the A/Brevig Mission/1918/1 (H1N1). We also show that A/Brevig Mission/1918/1 (H1N1) segment 8 mRNAs responded differently to overexpression of SR proteins than A/Netherlands/178/95 (H3N2). SN - 1096-0341 UR - https://www.unboundmedicine.com/medline/citation/22036312/Inefficient_splicing_of_segment_7_and_8_mRNAs_is_an_inherent_property_of_influenza_virus_A/Brevig_Mission/1918/1__H1N1__that_causes_elevated_expression_of_NS1_protein_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0042-6822(11)00472-7 DB - PRIME DP - Unbound Medicine ER -