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Effect of ultraviolet filters on skin superoxide dismutase activity in hairless mice after a single dose of ultraviolet radiation.
Eur J Pharm Biopharm. 2012 Feb; 80(2):387-92.EJ

Abstract

Organic sunscreens may decrease their protective capability and also behave as photo-oxidants upon ultraviolet radiation (UVR) exposure. The present study investigated the effect of a cream gel formulation containing the UV filters benzophenone-3, octyl methoxycinnamate, and octyl salicylate on skin superoxide dismutase (SOD) after a single dose of UVR (2.87 J/cm(2)). The retention of these UV filters was first evaluated in vivo using hairless mice to guarantee the presence of the filters in the skin layers at the moment of irradiation. The in vivo effect of the UV filters on skin SOD was then assayed spectrophotometrically via the reduction of cytochrome c. The cream gel formulation promoted the penetration of the three UV filters into the epidermis and the dermis at one hour post-application. A significant decrease in SOD activity was observed in irradiated animals treated with sunscreen formulation. However, no effect on SOD activity in skin was observed by the isolated presence of the sunscreens, the formulation components, or the exposure to UVR. The sunscreens may have formed degradation products under UVR that may have either inhibited the enzyme or generated reactive species in the skin.

Authors+Show Affiliations

Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, São Paulo, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22036989

Citation

Vilela, Fernanda Maria Pinto, et al. "Effect of Ultraviolet Filters On Skin Superoxide Dismutase Activity in Hairless Mice After a Single Dose of Ultraviolet Radiation." European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, vol. 80, no. 2, 2012, pp. 387-92.
Vilela FM, Fonseca YM, Jabor JR, et al. Effect of ultraviolet filters on skin superoxide dismutase activity in hairless mice after a single dose of ultraviolet radiation. Eur J Pharm Biopharm. 2012;80(2):387-92.
Vilela, F. M., Fonseca, Y. M., Jabor, J. R., Vicentini, F. T., & Fonseca, M. J. (2012). Effect of ultraviolet filters on skin superoxide dismutase activity in hairless mice after a single dose of ultraviolet radiation. European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Fur Pharmazeutische Verfahrenstechnik E.V, 80(2), 387-92. https://doi.org/10.1016/j.ejpb.2011.10.005
Vilela FM, et al. Effect of Ultraviolet Filters On Skin Superoxide Dismutase Activity in Hairless Mice After a Single Dose of Ultraviolet Radiation. Eur J Pharm Biopharm. 2012;80(2):387-92. PubMed PMID: 22036989.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of ultraviolet filters on skin superoxide dismutase activity in hairless mice after a single dose of ultraviolet radiation. AU - Vilela,Fernanda Maria Pinto, AU - Fonseca,Yris Maria, AU - Jabor,José Roberto, AU - Vicentini,Fabiana T M C, AU - Fonseca,Maria José Vieira, Y1 - 2011/10/20/ PY - 2011/03/29/received PY - 2011/07/25/revised PY - 2011/10/05/accepted PY - 2011/11/1/entrez PY - 2011/11/1/pubmed PY - 2012/5/23/medline SP - 387 EP - 92 JF - European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V JO - Eur J Pharm Biopharm VL - 80 IS - 2 N2 - Organic sunscreens may decrease their protective capability and also behave as photo-oxidants upon ultraviolet radiation (UVR) exposure. The present study investigated the effect of a cream gel formulation containing the UV filters benzophenone-3, octyl methoxycinnamate, and octyl salicylate on skin superoxide dismutase (SOD) after a single dose of UVR (2.87 J/cm(2)). The retention of these UV filters was first evaluated in vivo using hairless mice to guarantee the presence of the filters in the skin layers at the moment of irradiation. The in vivo effect of the UV filters on skin SOD was then assayed spectrophotometrically via the reduction of cytochrome c. The cream gel formulation promoted the penetration of the three UV filters into the epidermis and the dermis at one hour post-application. A significant decrease in SOD activity was observed in irradiated animals treated with sunscreen formulation. However, no effect on SOD activity in skin was observed by the isolated presence of the sunscreens, the formulation components, or the exposure to UVR. The sunscreens may have formed degradation products under UVR that may have either inhibited the enzyme or generated reactive species in the skin. SN - 1873-3441 UR - https://www.unboundmedicine.com/medline/citation/22036989/Effect_of_ultraviolet_filters_on_skin_superoxide_dismutase_activity_in_hairless_mice_after_a_single_dose_of_ultraviolet_radiation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0939-6411(11)00300-6 DB - PRIME DP - Unbound Medicine ER -