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Dispensable role for 4-1BB and 4-1BBL in development of vaccinia virus-specific CD8 T cells.
Immunol Lett. 2012 Jan 30; 141(2):220-6.IL

Abstract

CD8 T cells are strongly induced in response to certain strains of vaccinia virus (VACV) and the generation of this population is tightly regulated by two Tumor Necrosis Factor (TNF)/TNFR superfamily members, OX40 (CD134) and CD27. In this study, we examined the role of another member of the TNFR superfamily, 4-1BB (CD137, TNFRSF9), and its ligand (4-1BBL, CD137L, TNFSF9), that have been described to control the generation of memory CD8 T cell populations elicited by other viruses such as influenza. Expression of 4-1BB and 4-1BBL was observed in wild-type mice during the primary infection, but we found that both 4-1BB and 4-1BBL deficient mice generated normal numbers of VACV-specific effector CD8 T cells that produced IFN-γ and TNF. Additionally, CD8 T cells deficient in 4-1BB were able to expand and persist comparably to wild-type T cells in response to VACV infection. Furthermore, the knockout mice also showed no defect in development of VACV-specific CD8 memory T cell populations. Lastly, showing alternate control mechanisms were not active in the gene-deficient environments that masked any activity, blocking 4-1BB/4-1BBL interactions using neutralizing antibody also had no effect on the number of VACV-specific memory CD8 T cells induced. Thus, our data demonstrate that 4-1BB and 4-1BBL do not play a strong or dominant role in driving the generation of high frequencies of VACV-specific CD8 T cells.

Authors+Show Affiliations

La Jolla Institute for Allergy and Immunology, Division of Immune Regulation, 9420 Athena Circle, La Jolla, San Diego, CA 92037, USA.No affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

22037570

Citation

Zhao, Yuan, and Michael Croft. "Dispensable Role for 4-1BB and 4-1BBL in Development of Vaccinia Virus-specific CD8 T Cells." Immunology Letters, vol. 141, no. 2, 2012, pp. 220-6.
Zhao Y, Croft M. Dispensable role for 4-1BB and 4-1BBL in development of vaccinia virus-specific CD8 T cells. Immunol Lett. 2012;141(2):220-6.
Zhao, Y., & Croft, M. (2012). Dispensable role for 4-1BB and 4-1BBL in development of vaccinia virus-specific CD8 T cells. Immunology Letters, 141(2), 220-6. https://doi.org/10.1016/j.imlet.2011.10.008
Zhao Y, Croft M. Dispensable Role for 4-1BB and 4-1BBL in Development of Vaccinia Virus-specific CD8 T Cells. Immunol Lett. 2012 Jan 30;141(2):220-6. PubMed PMID: 22037570.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Dispensable role for 4-1BB and 4-1BBL in development of vaccinia virus-specific CD8 T cells. AU - Zhao,Yuan, AU - Croft,Michael, Y1 - 2011/10/20/ PY - 2011/08/01/received PY - 2011/09/30/revised PY - 2011/10/16/accepted PY - 2011/11/1/entrez PY - 2011/11/1/pubmed PY - 2012/4/24/medline SP - 220 EP - 6 JF - Immunology letters JO - Immunol. Lett. VL - 141 IS - 2 N2 - CD8 T cells are strongly induced in response to certain strains of vaccinia virus (VACV) and the generation of this population is tightly regulated by two Tumor Necrosis Factor (TNF)/TNFR superfamily members, OX40 (CD134) and CD27. In this study, we examined the role of another member of the TNFR superfamily, 4-1BB (CD137, TNFRSF9), and its ligand (4-1BBL, CD137L, TNFSF9), that have been described to control the generation of memory CD8 T cell populations elicited by other viruses such as influenza. Expression of 4-1BB and 4-1BBL was observed in wild-type mice during the primary infection, but we found that both 4-1BB and 4-1BBL deficient mice generated normal numbers of VACV-specific effector CD8 T cells that produced IFN-γ and TNF. Additionally, CD8 T cells deficient in 4-1BB were able to expand and persist comparably to wild-type T cells in response to VACV infection. Furthermore, the knockout mice also showed no defect in development of VACV-specific CD8 memory T cell populations. Lastly, showing alternate control mechanisms were not active in the gene-deficient environments that masked any activity, blocking 4-1BB/4-1BBL interactions using neutralizing antibody also had no effect on the number of VACV-specific memory CD8 T cells induced. Thus, our data demonstrate that 4-1BB and 4-1BBL do not play a strong or dominant role in driving the generation of high frequencies of VACV-specific CD8 T cells. SN - 1879-0542 UR - https://www.unboundmedicine.com/medline/citation/22037570/Dispensable_role_for_4_1BB_and_4_1BBL_in_development_of_vaccinia_virus_specific_CD8_T_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2478(11)00250-1 DB - PRIME DP - Unbound Medicine ER -