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Fine genetic mapping of greenbug aphid-resistance gene Gb3 in Aegilops tauschii.
Theor Appl Genet. 2012 Feb; 124(3):555-64.TA

Abstract

The greenbug, Schizaphis graminum (Rondani), is an important aphid pest of small grain crops especially wheat (Triticum aestivum L., 2n = 6x = 42, genomes AABBDD) in many parts of the world. The greenbug-resistance gene Gb3 originated from Aegilops tauschii Coss. (2n = 2x = 14, genome D(t)D(t)) has shown consistent and durable resistance against prevailing greenbug biotypes in wheat fields. We previously mapped Gb3 in a recombination-rich, telomeric bin of wheat chromosome arm 7DL. In this study, high-resolution genetic mapping was carried out using an F(2:3) segregating population derived from two Ae. tauschii accessions, the resistant PI 268210 (original donor of Gb3 in the hexaploid wheat germplasm line 'Largo') and susceptible AL8/78. Molecular markers were developed by exploring bin-mapped wheat RFLPs, SSRs, ESTs and the Ae. tauschii physical map (BAC contigs). Wheat EST and Ae. tauschii BAC end sequences located in the deletion bin 7DL3-0.82-1.00 were used to design STS (sequence tagged site) or CAPS (Cleaved Amplified Polymorphic Sequence) markers. Forty-five PCR-based markers were developed and mapped to the chromosomal region spanning the Gb3 locus. The greenbug-resistance gene Gb3 now was delimited in an interval of 1.1 cM by two molecular markers (HI067J6-R and HI009B3-R). This localized high-resolution genetic map with markers closely linked to Gb3 lays a solid foundation for map based cloning of Gb3 and marker-assisted selection of this gene in wheat breeding.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Azhaguvel P
Texas AgriLife Research, 6500 Amarillo Blvd W, Amarillo, TX 79106, USA. aperumal@noble.org
Rudd JC
No affiliation info available
Ma Y
No affiliation info available
Luo MC
No affiliation info available
Weng Y
No affiliation info available

MeSH

Amplified Fragment Length Polymorphism AnalysisAnimalsAphidsBreedingChromosome MappingChromosomes, Artificial, BacterialDisease ResistanceExpressed Sequence TagsGenes, PlantGenetic MarkersPlant DiseasesPoaceaeSequence Tagged Sites

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22038487

Citation

Azhaguvel, Perumal, et al. "Fine Genetic Mapping of Greenbug Aphid-resistance Gene Gb3 in Aegilops Tauschii." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 124, no. 3, 2012, pp. 555-64.
Azhaguvel P, Rudd JC, Ma Y, et al. Fine genetic mapping of greenbug aphid-resistance gene Gb3 in Aegilops tauschii. Theor Appl Genet. 2012;124(3):555-64.
Azhaguvel, P., Rudd, J. C., Ma, Y., Luo, M. C., & Weng, Y. (2012). Fine genetic mapping of greenbug aphid-resistance gene Gb3 in Aegilops tauschii. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 124(3), 555-64. https://doi.org/10.1007/s00122-011-1728-z
Azhaguvel P, et al. Fine Genetic Mapping of Greenbug Aphid-resistance Gene Gb3 in Aegilops Tauschii. Theor Appl Genet. 2012;124(3):555-64. PubMed PMID: 22038487.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fine genetic mapping of greenbug aphid-resistance gene Gb3 in Aegilops tauschii. AU - Azhaguvel,Perumal, AU - Rudd,Jackie C, AU - Ma,Yaqin, AU - Luo,Ming-Cheng, AU - Weng,Yiqun, Y1 - 2011/10/30/ PY - 2011/06/28/received PY - 2011/10/07/accepted PY - 2011/11/1/entrez PY - 2011/11/1/pubmed PY - 2012/5/15/medline SP - 555 EP - 64 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 124 IS - 3 N2 - The greenbug, Schizaphis graminum (Rondani), is an important aphid pest of small grain crops especially wheat (Triticum aestivum L., 2n = 6x = 42, genomes AABBDD) in many parts of the world. The greenbug-resistance gene Gb3 originated from Aegilops tauschii Coss. (2n = 2x = 14, genome D(t)D(t)) has shown consistent and durable resistance against prevailing greenbug biotypes in wheat fields. We previously mapped Gb3 in a recombination-rich, telomeric bin of wheat chromosome arm 7DL. In this study, high-resolution genetic mapping was carried out using an F(2:3) segregating population derived from two Ae. tauschii accessions, the resistant PI 268210 (original donor of Gb3 in the hexaploid wheat germplasm line 'Largo') and susceptible AL8/78. Molecular markers were developed by exploring bin-mapped wheat RFLPs, SSRs, ESTs and the Ae. tauschii physical map (BAC contigs). Wheat EST and Ae. tauschii BAC end sequences located in the deletion bin 7DL3-0.82-1.00 were used to design STS (sequence tagged site) or CAPS (Cleaved Amplified Polymorphic Sequence) markers. Forty-five PCR-based markers were developed and mapped to the chromosomal region spanning the Gb3 locus. The greenbug-resistance gene Gb3 now was delimited in an interval of 1.1 cM by two molecular markers (HI067J6-R and HI009B3-R). This localized high-resolution genetic map with markers closely linked to Gb3 lays a solid foundation for map based cloning of Gb3 and marker-assisted selection of this gene in wheat breeding. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/22038487/Fine_genetic_mapping_of_greenbug_aphid_resistance_gene_Gb3_in_Aegilops_tauschii_ L2 - https://dx.doi.org/10.1007/s00122-011-1728-z DB - PRIME DP - Unbound Medicine ER -