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Skin colonization by superantigen-producing Staphylococcus aureus in Egyptian patients with atopic dermatitis and its relation to disease severity and serum interleukin-4 level.
Int J Infect Dis. 2012 Jan; 16(1):e29-33.IJ

Abstract

OBJECTIVE

The objective of the current study was to detect Staphylococcus aureus colonization and the presence of superantigen in atopic dermatitis (AD) in Egyptian patients, and to determine its effect on disease severity and serum interleukin (IL)-4 levels.

METHODS

Swabs were taken from the skin of 30 patients with AD. S. aureus isolates were screened for the presence of genes of exotoxins with superantigen properties by multiplex PCR. Serum IL-4 was determined by ELISA. The rate of S. aureus colonization and the presence of superantigen were correlated with disease severity and IL-4 level.

RESULTS

Twenty-six of 30 patients (87%) were colonized by S. aureus, and 14 of the 26 (54%) patients were colonized with toxigenic strains. The most frequent superantigen gene present in S. aureus isolates was that coding for staphylococcal enterotoxin B (SEB), followed by both staphylococcal enterotoxin C (SEC) and toxic shock syndrome toxin-1 (TSST-1) genes. The mean 'severity scoring in AD' (SCORAD) score of AD patients colonized with S. aureus harboring superantigen genes (74 ± 8) was significantly higher than that in those colonized with S. aureus isolates without superantigen genes (56 ± 6) (p<0.001). Serum IL-4 levels followed the same pattern.

CONCLUSIONS

S. aureus may play an important role as an aggravating factor in AD patients. Reducing the colonization of atopic skin by S. aureus is therefore the best way to reduce superantigen-induced allergic skin inflammation.

Authors+Show Affiliations

Department of Dermatology and Venereology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22040925

Citation

Nada, Hesham A., et al. "Skin Colonization By Superantigen-producing Staphylococcus Aureus in Egyptian Patients With Atopic Dermatitis and Its Relation to Disease Severity and Serum Interleukin-4 Level." International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, vol. 16, no. 1, 2012, pp. e29-33.
Nada HA, Gomaa NI, Elakhras A, et al. Skin colonization by superantigen-producing Staphylococcus aureus in Egyptian patients with atopic dermatitis and its relation to disease severity and serum interleukin-4 level. Int J Infect Dis. 2012;16(1):e29-33.
Nada, H. A., Gomaa, N. I., Elakhras, A., Wasfy, R., & Baker, R. A. (2012). Skin colonization by superantigen-producing Staphylococcus aureus in Egyptian patients with atopic dermatitis and its relation to disease severity and serum interleukin-4 level. International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases, 16(1), e29-33. https://doi.org/10.1016/j.ijid.2011.09.014
Nada HA, et al. Skin Colonization By Superantigen-producing Staphylococcus Aureus in Egyptian Patients With Atopic Dermatitis and Its Relation to Disease Severity and Serum Interleukin-4 Level. Int J Infect Dis. 2012;16(1):e29-33. PubMed PMID: 22040925.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Skin colonization by superantigen-producing Staphylococcus aureus in Egyptian patients with atopic dermatitis and its relation to disease severity and serum interleukin-4 level. AU - Nada,Hesham A, AU - Gomaa,Nahed I M, AU - Elakhras,Atef, AU - Wasfy,Roshdy, AU - Baker,Rabee Abu, Y1 - 2011/10/30/ PY - 2011/05/26/received PY - 2011/07/24/revised PY - 2011/09/15/accepted PY - 2011/11/2/entrez PY - 2011/11/2/pubmed PY - 2012/4/28/medline SP - e29 EP - 33 JF - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases JO - Int J Infect Dis VL - 16 IS - 1 N2 - OBJECTIVE: The objective of the current study was to detect Staphylococcus aureus colonization and the presence of superantigen in atopic dermatitis (AD) in Egyptian patients, and to determine its effect on disease severity and serum interleukin (IL)-4 levels. METHODS: Swabs were taken from the skin of 30 patients with AD. S. aureus isolates were screened for the presence of genes of exotoxins with superantigen properties by multiplex PCR. Serum IL-4 was determined by ELISA. The rate of S. aureus colonization and the presence of superantigen were correlated with disease severity and IL-4 level. RESULTS: Twenty-six of 30 patients (87%) were colonized by S. aureus, and 14 of the 26 (54%) patients were colonized with toxigenic strains. The most frequent superantigen gene present in S. aureus isolates was that coding for staphylococcal enterotoxin B (SEB), followed by both staphylococcal enterotoxin C (SEC) and toxic shock syndrome toxin-1 (TSST-1) genes. The mean 'severity scoring in AD' (SCORAD) score of AD patients colonized with S. aureus harboring superantigen genes (74 ± 8) was significantly higher than that in those colonized with S. aureus isolates without superantigen genes (56 ± 6) (p<0.001). Serum IL-4 levels followed the same pattern. CONCLUSIONS: S. aureus may play an important role as an aggravating factor in AD patients. Reducing the colonization of atopic skin by S. aureus is therefore the best way to reduce superantigen-induced allergic skin inflammation. SN - 1878-3511 UR - https://www.unboundmedicine.com/medline/citation/22040925/Skin_colonization_by_superantigen_producing_Staphylococcus_aureus_in_Egyptian_patients_with_atopic_dermatitis_and_its_relation_to_disease_severity_and_serum_interleukin_4_level_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1201-9712(11)00193-7 DB - PRIME DP - Unbound Medicine ER -