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[Effects of NF-kappaB on the expression of IL-8 in human bronchial epithelial cells induced by cigarette smoke condensates].
Wei Sheng Yan Jiu. 2011 Sep; 40(5):573-5.WS

Abstract

OBJECTIVE

To investigate the effects of NF-kappaB on the expression of IL-8 in human bronchial epithelial cells induced by cigarette smoke condensates (CSC).

METHODS

Both IL-8mNF-kappaB(-) BEAS-2B cells (BEAS-2B cells with mutated NF-kappaB binding site in IL-8 promoter) and IL-8 WT BEAS-2B cells (wild-type BEAS-2B cells with completed NF-kappaB binding site in IL-8 promoter) were treated by 7.5% CSC. The mRNA level of fLCF gene was detected by real-time PCR, and the activity of luciferase was measured by luciferase reporter gene activity detection kit.

RESULTS

After two kinds of BEAS-2B cells were treated by 7.5% CSC, the mRNA level of fLCF and the activity of luciferase in IL-8 WT BEAS-2B cells was higher than those in IL-8mNF-kappaB(-) BEAS-2B cells (P < 0.001), the mRNA level of fLCF in IL-8 WT BEAS-2B cells was 1.32 times as that in IL-8mNF-kappaB(-) BEAS-2B cells (P < 0.001).

CONCLUSION

The results suggested that NF-kappaB transcription factor was involved in smoking-induced respiratory inflammation through the precise molecular mechanisms of promoting the release of IL-8 in BEAS-2B cells treated by CSC.

Authors+Show Affiliations

Department of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China. zhangqiao@zzu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

22043704

Citation

Zhang, Qiao, et al. "[Effects of NF-kappaB On the Expression of IL-8 in Human Bronchial Epithelial Cells Induced By Cigarette Smoke Condensates]." Wei Sheng Yan Jiu = Journal of Hygiene Research, vol. 40, no. 5, 2011, pp. 573-5.
Zhang Q, Cheng L, Xing R, et al. [Effects of NF-kappaB on the expression of IL-8 in human bronchial epithelial cells induced by cigarette smoke condensates]. Wei Sheng Yan Jiu. 2011;40(5):573-5.
Zhang, Q., Cheng, L., Xing, R., Ma, L., Li, C., Xu, Y., & Wu, W. (2011). [Effects of NF-kappaB on the expression of IL-8 in human bronchial epithelial cells induced by cigarette smoke condensates]. Wei Sheng Yan Jiu = Journal of Hygiene Research, 40(5), 573-5.
Zhang Q, et al. [Effects of NF-kappaB On the Expression of IL-8 in Human Bronchial Epithelial Cells Induced By Cigarette Smoke Condensates]. Wei Sheng Yan Jiu. 2011;40(5):573-5. PubMed PMID: 22043704.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of NF-kappaB on the expression of IL-8 in human bronchial epithelial cells induced by cigarette smoke condensates]. AU - Zhang,Qiao, AU - Cheng,Luming, AU - Xing,Ruiting, AU - Ma,Lu, AU - Li,Chunyang, AU - Xu,Yubao, AU - Wu,Weidong, PY - 2011/11/3/entrez PY - 2011/11/3/pubmed PY - 2013/3/15/medline SP - 573 EP - 5 JF - Wei sheng yan jiu = Journal of hygiene research JO - Wei Sheng Yan Jiu VL - 40 IS - 5 N2 - OBJECTIVE: To investigate the effects of NF-kappaB on the expression of IL-8 in human bronchial epithelial cells induced by cigarette smoke condensates (CSC). METHODS: Both IL-8mNF-kappaB(-) BEAS-2B cells (BEAS-2B cells with mutated NF-kappaB binding site in IL-8 promoter) and IL-8 WT BEAS-2B cells (wild-type BEAS-2B cells with completed NF-kappaB binding site in IL-8 promoter) were treated by 7.5% CSC. The mRNA level of fLCF gene was detected by real-time PCR, and the activity of luciferase was measured by luciferase reporter gene activity detection kit. RESULTS: After two kinds of BEAS-2B cells were treated by 7.5% CSC, the mRNA level of fLCF and the activity of luciferase in IL-8 WT BEAS-2B cells was higher than those in IL-8mNF-kappaB(-) BEAS-2B cells (P < 0.001), the mRNA level of fLCF in IL-8 WT BEAS-2B cells was 1.32 times as that in IL-8mNF-kappaB(-) BEAS-2B cells (P < 0.001). CONCLUSION: The results suggested that NF-kappaB transcription factor was involved in smoking-induced respiratory inflammation through the precise molecular mechanisms of promoting the release of IL-8 in BEAS-2B cells treated by CSC. SN - 1000-8020 UR - https://www.unboundmedicine.com/medline/citation/22043704/[Effects_of_NF_kappaB_on_the_expression_of_IL_8_in_human_bronchial_epithelial_cells_induced_by_cigarette_smoke_condensates]_ DB - PRIME DP - Unbound Medicine ER -