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Bacterial communities in sediments of the shallow Lake Dongping in China.
J Appl Microbiol. 2012 Jan; 112(1):79-89.JA

Abstract

AIMS

The purpose of this study was to discuss how the environmental inputs and anthropogenic activities impact bacterial communities in the sediments of a shallow, eutrophic and temperate freshwater lake.

METHODS AND RESULTS

Sediment cores were collected from Lake Dongping, located in Taian, Shandong, China. All samples were processed within 4 h of collection. Total nitrogen, total phosphorus (TP), total organic carbon, ammonium nitrogen and nitrate nitrogen content of samples were measured by Kjeldahl determination, sulphuric acid-perchloric acid digestion and molybdenum blue colorimetry, potassium dichromate titration, Nessler's reagent colorimetric and the phenol disulphonic acid colorimetric method, respectively. Seasonal and temporal diversity of sediment bacterial communities at six stations in Lake Dongping were investigated using molecular approaches (terminal restriction fragment length polymorphism and 16S rDNA clone libraries). Noticeable seasonal and temporal variations were observed in bacterial diversity and composition at all six stations. Sediment bacterial communities in Lake Dongping belonged to 16 phyla: Proteobacteria (including α-Proteobacteria, β-Proteobacteria, δ-Proteobacteria, ε-Proteobacteria, γ-Proteobacteria), Acidobacteria, Planctomycetes, Bacteroidetes, Firmicutes, Verrucomicrobia, Nitrospira, Chloroflexi, Gemmatimonadetes, Chlorobi, Cyanobacteria, Deferribacteres, Actinobacteria, OP8, Spirochaetes and OP11. Members of β-, δ- and γ-Proteobacterial sequences were predominant in 11 of 12 clone libraries derived from sediment samples. Sediment samples collected at stations 1 and 4 in July had the greatest bacterial diversity while those collected at station 2 in October had the least diversity. TP concentration was significantly correlated with the distribution of bacterial communities.

CONCLUSIONS

Our results suggested that different environmental nutrient inputs contribute to seasonal and temporal variations of chemical features and bacterial communities in sediments of Lake Dongping. TP concentration was significantly correlated with the distribution of bacterial communities.

SIGNIFICANCE AND IMPACT OF THE STUDY

This study has an important implication for the optimization of integrated ecosystem assessment of shallow temperate freshwater lake and provides interesting information for the subsequent of the ecosystem.

Authors+Show Affiliations

Department of Microbiology, College of Life Science, Shandong Agricultural University, Taian, Shandong Province, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22044641

Citation

Song, H, et al. "Bacterial Communities in Sediments of the Shallow Lake Dongping in China." Journal of Applied Microbiology, vol. 112, no. 1, 2012, pp. 79-89.
Song H, Li Z, Du B, et al. Bacterial communities in sediments of the shallow Lake Dongping in China. J Appl Microbiol. 2012;112(1):79-89.
Song, H., Li, Z., Du, B., Wang, G., & Ding, Y. (2012). Bacterial communities in sediments of the shallow Lake Dongping in China. Journal of Applied Microbiology, 112(1), 79-89. https://doi.org/10.1111/j.1365-2672.2011.05187.x
Song H, et al. Bacterial Communities in Sediments of the Shallow Lake Dongping in China. J Appl Microbiol. 2012;112(1):79-89. PubMed PMID: 22044641.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bacterial communities in sediments of the shallow Lake Dongping in China. AU - Song,H, AU - Li,Z, AU - Du,B, AU - Wang,G, AU - Ding,Y, Y1 - 2011/11/24/ PY - 2011/11/3/entrez PY - 2011/11/3/pubmed PY - 2012/4/11/medline SP - 79 EP - 89 JF - Journal of applied microbiology JO - J Appl Microbiol VL - 112 IS - 1 N2 - AIMS: The purpose of this study was to discuss how the environmental inputs and anthropogenic activities impact bacterial communities in the sediments of a shallow, eutrophic and temperate freshwater lake. METHODS AND RESULTS: Sediment cores were collected from Lake Dongping, located in Taian, Shandong, China. All samples were processed within 4 h of collection. Total nitrogen, total phosphorus (TP), total organic carbon, ammonium nitrogen and nitrate nitrogen content of samples were measured by Kjeldahl determination, sulphuric acid-perchloric acid digestion and molybdenum blue colorimetry, potassium dichromate titration, Nessler's reagent colorimetric and the phenol disulphonic acid colorimetric method, respectively. Seasonal and temporal diversity of sediment bacterial communities at six stations in Lake Dongping were investigated using molecular approaches (terminal restriction fragment length polymorphism and 16S rDNA clone libraries). Noticeable seasonal and temporal variations were observed in bacterial diversity and composition at all six stations. Sediment bacterial communities in Lake Dongping belonged to 16 phyla: Proteobacteria (including α-Proteobacteria, β-Proteobacteria, δ-Proteobacteria, ε-Proteobacteria, γ-Proteobacteria), Acidobacteria, Planctomycetes, Bacteroidetes, Firmicutes, Verrucomicrobia, Nitrospira, Chloroflexi, Gemmatimonadetes, Chlorobi, Cyanobacteria, Deferribacteres, Actinobacteria, OP8, Spirochaetes and OP11. Members of β-, δ- and γ-Proteobacterial sequences were predominant in 11 of 12 clone libraries derived from sediment samples. Sediment samples collected at stations 1 and 4 in July had the greatest bacterial diversity while those collected at station 2 in October had the least diversity. TP concentration was significantly correlated with the distribution of bacterial communities. CONCLUSIONS: Our results suggested that different environmental nutrient inputs contribute to seasonal and temporal variations of chemical features and bacterial communities in sediments of Lake Dongping. TP concentration was significantly correlated with the distribution of bacterial communities. SIGNIFICANCE AND IMPACT OF THE STUDY: This study has an important implication for the optimization of integrated ecosystem assessment of shallow temperate freshwater lake and provides interesting information for the subsequent of the ecosystem. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/22044641/Bacterial_communities_in_sediments_of_the_shallow_Lake_Dongping_in_China_ L2 - https://doi.org/10.1111/j.1365-2672.2011.05187.x DB - PRIME DP - Unbound Medicine ER -