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Pyrazinecarboxamides as potential elicitors of flavonolignan and flavonoid production in Silybum marianum and Ononis arvensis cultures in vitro.
Molecules 2011; 16(11):9142-52M

Abstract

The effect of new synthetic pyrazinecarboxamide derivatives as potential elicitors of flavonolignan and flavonoid production in Silybum marianum and Ononis arvensis cultures in vitro was investigated. Both tested elicitors increased the production of flavonolignans in S. marianum callus and suspension cultures and flavonoids in O. arvensis callus and suspension cultures. Compound I, 5-(2-hydroxybenzoyl)-pyrazine-2-carboxamide, has shown to be an effective elicitor of flavonolignans and taxifoline production in Silybum marianum culture in vitro. The maximum content of silydianin (0.11%) in S. marianum suspension culture was induced by 24 h elicitor application in concentration of 1.159 × 10⁻³ mol/L. The maximum content of silymarin complex (0.08%) in callus culture of S. marianum was induced by 168 h elicitor application of a concentration 1.159 × 10⁻⁴ mol/L, which represents contents of silydianin (0.03%), silychristin (0.01%) and isosilybin A (0.04%) compared with control. All three tested concentrations of compound II, N-(2-bromo-3-methylphenyl)-5-tert-butylpyrazin-2-carboxamide increased the flavonoid production in callus culture of O. arvensis in a statistically significant way. The best elicitation effect of all elicitor concentrations had the weakest c₃ concentration (8.36 × 10⁻⁶ mol/L) after 168 h time of duration. The maximum content of flavonoids (about 5,900%) in suspension culture of O. arvensis was induced by 48 h application of c₃ concentration (8.36 × 10⁻⁶ mol/L).

Authors+Show Affiliations

Department of Pharmacognosy, Faculty of Pharmacy in Hradec Kralove, Charles University in Prague, Heyrovskeho 1203, 50005 Hradec Kralove, Czech Republic. tumova@faf.cuni.czNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22045042

Citation

Tumova, Lenka, et al. "Pyrazinecarboxamides as Potential Elicitors of Flavonolignan and Flavonoid Production in Silybum Marianum and Ononis Arvensis Cultures in Vitro." Molecules (Basel, Switzerland), vol. 16, no. 11, 2011, pp. 9142-52.
Tumova L, Tuma J, Dolezal M. Pyrazinecarboxamides as potential elicitors of flavonolignan and flavonoid production in Silybum marianum and Ononis arvensis cultures in vitro. Molecules. 2011;16(11):9142-52.
Tumova, L., Tuma, J., & Dolezal, M. (2011). Pyrazinecarboxamides as potential elicitors of flavonolignan and flavonoid production in Silybum marianum and Ononis arvensis cultures in vitro. Molecules (Basel, Switzerland), 16(11), pp. 9142-52. doi:10.3390/molecules16119142.
Tumova L, Tuma J, Dolezal M. Pyrazinecarboxamides as Potential Elicitors of Flavonolignan and Flavonoid Production in Silybum Marianum and Ononis Arvensis Cultures in Vitro. Molecules. 2011 Nov 1;16(11):9142-52. PubMed PMID: 22045042.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Pyrazinecarboxamides as potential elicitors of flavonolignan and flavonoid production in Silybum marianum and Ononis arvensis cultures in vitro. AU - Tumova,Lenka, AU - Tuma,Jiri, AU - Dolezal,Martin, Y1 - 2011/11/01/ PY - 2011/09/29/received PY - 2011/10/25/revised PY - 2011/10/28/accepted PY - 2011/11/3/entrez PY - 2011/11/3/pubmed PY - 2012/4/14/medline SP - 9142 EP - 52 JF - Molecules (Basel, Switzerland) JO - Molecules VL - 16 IS - 11 N2 - The effect of new synthetic pyrazinecarboxamide derivatives as potential elicitors of flavonolignan and flavonoid production in Silybum marianum and Ononis arvensis cultures in vitro was investigated. Both tested elicitors increased the production of flavonolignans in S. marianum callus and suspension cultures and flavonoids in O. arvensis callus and suspension cultures. Compound I, 5-(2-hydroxybenzoyl)-pyrazine-2-carboxamide, has shown to be an effective elicitor of flavonolignans and taxifoline production in Silybum marianum culture in vitro. The maximum content of silydianin (0.11%) in S. marianum suspension culture was induced by 24 h elicitor application in concentration of 1.159 × 10⁻³ mol/L. The maximum content of silymarin complex (0.08%) in callus culture of S. marianum was induced by 168 h elicitor application of a concentration 1.159 × 10⁻⁴ mol/L, which represents contents of silydianin (0.03%), silychristin (0.01%) and isosilybin A (0.04%) compared with control. All three tested concentrations of compound II, N-(2-bromo-3-methylphenyl)-5-tert-butylpyrazin-2-carboxamide increased the flavonoid production in callus culture of O. arvensis in a statistically significant way. The best elicitation effect of all elicitor concentrations had the weakest c₃ concentration (8.36 × 10⁻⁶ mol/L) after 168 h time of duration. The maximum content of flavonoids (about 5,900%) in suspension culture of O. arvensis was induced by 48 h application of c₃ concentration (8.36 × 10⁻⁶ mol/L). SN - 1420-3049 UR - https://www.unboundmedicine.com/medline/citation/22045042/Pyrazinecarboxamides_as_potential_elicitors_of_flavonolignan_and_flavonoid_production_in_Silybum_marianum_and_Ononis_arvensis_cultures_in_vitro_ L2 - http://www.mdpi.com/resolver?pii=molecules16119142 DB - PRIME DP - Unbound Medicine ER -