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Simultaneous detection of seventeen drugs of abuse and metabolites in hair using solid phase micro extraction (SPME) with GC/MS.
Forensic Sci Int. 2012 May 10; 218(1-3):31-6.FS

Abstract

INTRODUCTION

The analysis of pediatric and adult hair is a useful non-invasive biomarker to effectively detect long term exposure to various xenobiotics, specifically drugs of abuse such as cocaine, opiates and amphetamines. Very often individuals are using, or are exposed to multiple drugs simultaneously and therefore it is important to be able to detect them in the same analysis. We have developed a sensitive and specific solid phase micro extraction (SPME) coupled with gas chromatography mass spectrometry (GC/MS) to detect 17 different analytes in hair using a single extraction method.

METHOD

Five milligrams of hair is extracted overnight, subjected to solid phase extraction (SPE) and then to SPME-GC/MS. The aimed analytes include amphetamine, methamphetamine, MDA, MDMA, cocaine, benzoylecognine, norcocaine, cocaethylene, methadone, codeine, morphine, 6-AM, oxycodone, oxymorphone, hydrocodone, hydromorphone and meperidone.

RESULTS

The following are the LOD of the various drugs: 0.2ng/mg hair for amphetamine, methamphetamine, MDA, MDMA, morphine, codeine, 6-AM, oxycodone, oxymorphone, hydromorphone, hydrocodone, meperidine and 0.13ng/mg hair for cocaine, benzoylecognine, cocaethylene, norcocaine and methadone.

CONCLUSION

This GC/MS method is sensitive and specific to detect the presence of these 17 analytes in as little as 5mg of hair and is especially useful for newborn and child hair analysis where the amount of hair is often very limited.

Authors+Show Affiliations

Hospital for Sick Children, Toronto, Canada.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22047752

Citation

Aleksa, Katarina, et al. "Simultaneous Detection of Seventeen Drugs of Abuse and Metabolites in Hair Using Solid Phase Micro Extraction (SPME) With GC/MS." Forensic Science International, vol. 218, no. 1-3, 2012, pp. 31-6.
Aleksa K, Walasek P, Fulga N, et al. Simultaneous detection of seventeen drugs of abuse and metabolites in hair using solid phase micro extraction (SPME) with GC/MS. Forensic Sci Int. 2012;218(1-3):31-6.
Aleksa, K., Walasek, P., Fulga, N., Kapur, B., Gareri, J., & Koren, G. (2012). Simultaneous detection of seventeen drugs of abuse and metabolites in hair using solid phase micro extraction (SPME) with GC/MS. Forensic Science International, 218(1-3), 31-6. https://doi.org/10.1016/j.forsciint.2011.10.002
Aleksa K, et al. Simultaneous Detection of Seventeen Drugs of Abuse and Metabolites in Hair Using Solid Phase Micro Extraction (SPME) With GC/MS. Forensic Sci Int. 2012 May 10;218(1-3):31-6. PubMed PMID: 22047752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous detection of seventeen drugs of abuse and metabolites in hair using solid phase micro extraction (SPME) with GC/MS. AU - Aleksa,Katarina, AU - Walasek,Paula, AU - Fulga,Netta, AU - Kapur,Bhushan, AU - Gareri,Joey, AU - Koren,Gideon, Y1 - 2011/11/01/ PY - 2011/04/05/received PY - 2011/05/31/accepted PY - 2011/11/4/entrez PY - 2011/11/4/pubmed PY - 2012/9/26/medline SP - 31 EP - 6 JF - Forensic science international JO - Forensic Sci Int VL - 218 IS - 1-3 N2 - INTRODUCTION: The analysis of pediatric and adult hair is a useful non-invasive biomarker to effectively detect long term exposure to various xenobiotics, specifically drugs of abuse such as cocaine, opiates and amphetamines. Very often individuals are using, or are exposed to multiple drugs simultaneously and therefore it is important to be able to detect them in the same analysis. We have developed a sensitive and specific solid phase micro extraction (SPME) coupled with gas chromatography mass spectrometry (GC/MS) to detect 17 different analytes in hair using a single extraction method. METHOD: Five milligrams of hair is extracted overnight, subjected to solid phase extraction (SPE) and then to SPME-GC/MS. The aimed analytes include amphetamine, methamphetamine, MDA, MDMA, cocaine, benzoylecognine, norcocaine, cocaethylene, methadone, codeine, morphine, 6-AM, oxycodone, oxymorphone, hydrocodone, hydromorphone and meperidone. RESULTS: The following are the LOD of the various drugs: 0.2ng/mg hair for amphetamine, methamphetamine, MDA, MDMA, morphine, codeine, 6-AM, oxycodone, oxymorphone, hydromorphone, hydrocodone, meperidine and 0.13ng/mg hair for cocaine, benzoylecognine, cocaethylene, norcocaine and methadone. CONCLUSION: This GC/MS method is sensitive and specific to detect the presence of these 17 analytes in as little as 5mg of hair and is especially useful for newborn and child hair analysis where the amount of hair is often very limited. SN - 1872-6283 UR - https://www.unboundmedicine.com/medline/citation/22047752/Simultaneous_detection_of_seventeen_drugs_of_abuse_and_metabolites_in_hair_using_solid_phase_micro_extraction__SPME__with_GC/MS_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0379-0738(11)00477-4 DB - PRIME DP - Unbound Medicine ER -