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A549 and PLC/PRF/5 cells can support the efficient propagation of swine and wild boar hepatitis E virus (HEV) strains: demonstration of HEV infectivity of porcine liver sold as food.
Arch Virol. 2012 Feb; 157(2):235-46.AV

Abstract

Recent evidence has indicated the cross-species transmission of hepatitis E virus (HEV) from pigs and wild boars to humans, causing zoonosis, mostly via consumption of uncooked or undercooked animal meat/viscera. However, no efficient cell culture system for swine and boar HEV strains has been established. We inoculated A549 cells with 12 swine and boar HEV strains of liver, feces, or serum origin at an HEV load of ≥2.0 × 10(4) copies per well and found that the HEV progeny replicated as efficiently as human HEV strains, with a maximum load of ~10(8) copies/ml. However, the HEV load in the culture medium at 30 days post-inoculation differed markedly by inoculum, ranging from 1.0 × 10(2) to 1.1 × 10(7) copies/ml upon inoculation at a lower load of approximately 10(5) copies per well. All progeny were passaged successfully onto A549 and PLC/PRF/5 cells. In sharp contrast, no progeny viruses were detectable in the culture supernatant upon inoculation with 13 swine and boar HEV strains at an HEV load of <1.8 × 10(4) copies per well. The present study also demonstrates that swine liver sold as food can be infectious, supporting the risk of zoonotic food-borne HEV infection.

Authors+Show Affiliations

Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22048607

Citation

Takahashi, Hideyuki, et al. "A549 and PLC/PRF/5 Cells Can Support the Efficient Propagation of Swine and Wild Boar Hepatitis E Virus (HEV) Strains: Demonstration of HEV Infectivity of Porcine Liver Sold as Food." Archives of Virology, vol. 157, no. 2, 2012, pp. 235-46.
Takahashi H, Tanaka T, Jirintai S, et al. A549 and PLC/PRF/5 cells can support the efficient propagation of swine and wild boar hepatitis E virus (HEV) strains: demonstration of HEV infectivity of porcine liver sold as food. Arch Virol. 2012;157(2):235-46.
Takahashi, H., Tanaka, T., Jirintai, S., Nagashima, S., Takahashi, M., Nishizawa, T., Mizuo, H., Yazaki, Y., & Okamoto, H. (2012). A549 and PLC/PRF/5 cells can support the efficient propagation of swine and wild boar hepatitis E virus (HEV) strains: demonstration of HEV infectivity of porcine liver sold as food. Archives of Virology, 157(2), 235-46. https://doi.org/10.1007/s00705-011-1153-2
Takahashi H, et al. A549 and PLC/PRF/5 Cells Can Support the Efficient Propagation of Swine and Wild Boar Hepatitis E Virus (HEV) Strains: Demonstration of HEV Infectivity of Porcine Liver Sold as Food. Arch Virol. 2012;157(2):235-46. PubMed PMID: 22048607.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A549 and PLC/PRF/5 cells can support the efficient propagation of swine and wild boar hepatitis E virus (HEV) strains: demonstration of HEV infectivity of porcine liver sold as food. AU - Takahashi,Hideyuki, AU - Tanaka,Toshinori, AU - Jirintai,Suljid, AU - Nagashima,Shigeo, AU - Takahashi,Masaharu, AU - Nishizawa,Tsutomu, AU - Mizuo,Hitoshi, AU - Yazaki,Yasuyuki, AU - Okamoto,Hiroaki, Y1 - 2011/11/03/ PY - 2011/09/08/received PY - 2011/10/15/accepted PY - 2011/11/4/entrez PY - 2011/11/4/pubmed PY - 2012/3/14/medline SP - 235 EP - 46 JF - Archives of virology JO - Arch Virol VL - 157 IS - 2 N2 - Recent evidence has indicated the cross-species transmission of hepatitis E virus (HEV) from pigs and wild boars to humans, causing zoonosis, mostly via consumption of uncooked or undercooked animal meat/viscera. However, no efficient cell culture system for swine and boar HEV strains has been established. We inoculated A549 cells with 12 swine and boar HEV strains of liver, feces, or serum origin at an HEV load of ≥2.0 × 10(4) copies per well and found that the HEV progeny replicated as efficiently as human HEV strains, with a maximum load of ~10(8) copies/ml. However, the HEV load in the culture medium at 30 days post-inoculation differed markedly by inoculum, ranging from 1.0 × 10(2) to 1.1 × 10(7) copies/ml upon inoculation at a lower load of approximately 10(5) copies per well. All progeny were passaged successfully onto A549 and PLC/PRF/5 cells. In sharp contrast, no progeny viruses were detectable in the culture supernatant upon inoculation with 13 swine and boar HEV strains at an HEV load of <1.8 × 10(4) copies per well. The present study also demonstrates that swine liver sold as food can be infectious, supporting the risk of zoonotic food-borne HEV infection. SN - 1432-8798 UR - https://www.unboundmedicine.com/medline/citation/22048607/A549_and_PLC/PRF/5_cells_can_support_the_efficient_propagation_of_swine_and_wild_boar_hepatitis_E_virus__HEV__strains:_demonstration_of_HEV_infectivity_of_porcine_liver_sold_as_food_ L2 - https://dx.doi.org/10.1007/s00705-011-1153-2 DB - PRIME DP - Unbound Medicine ER -