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A rapid method for detection of genetically modified organisms based on magnetic separation and surface-enhanced Raman scattering.
Analyst. 2012 Jan 07; 137(1):202-8.A

Abstract

In this study, a new method combining magnetic separation (MS) and surface-enhanced Raman scattering (SERS) was developed to detect genetically modified organisms (GMOs). An oligonucleotide probe which is specific for 35 S DNA target was immobilized onto gold coated magnetic nanospheres to form oligonucleotide-coated nanoparticles. A self assembled monolayer was formed on gold nanorods using 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) and the second probe of the 35 S DNA target was immobilized on the activated nanorod surfaces. Probes on the nanoparticles were hybridized with the target oligonucleotide. Optimization parameters for hybridization were investigated by high performance liquid chromatography. Optimum hybridization parameters were determined as: 4 μM probe concentration, 20 min immobilization time, 30 min hybridization time, 55 °C hybridization temperature, 750 mM buffer salt concentration and pH: 7.4. Quantification of the target concentration was performed via SERS spectra of DTNB on the nanorods. The correlation between the target concentration and the SERS signal was found to be linear within the range of 25-100 nM. The analyses were performed with only one hybridization step in 40 min. Real sample analysis was conducted using Bt-176 maize sample. The results showed that the developed MS-SERS assay is capable of detecting GMOs in a rapid and selective manner.

Authors+Show Affiliations

Department of Food Engineering, Faculty of Engineering, Hacettepe University, Beytepe, Ankara, 06800, Turkey.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22049365

Citation

Guven, Burcu, et al. "A Rapid Method for Detection of Genetically Modified Organisms Based On Magnetic Separation and Surface-enhanced Raman Scattering." The Analyst, vol. 137, no. 1, 2012, pp. 202-8.
Guven B, Boyacı İH, Tamer U, et al. A rapid method for detection of genetically modified organisms based on magnetic separation and surface-enhanced Raman scattering. Analyst. 2012;137(1):202-8.
Guven, B., Boyacı, İ. H., Tamer, U., & Çalık, P. (2012). A rapid method for detection of genetically modified organisms based on magnetic separation and surface-enhanced Raman scattering. The Analyst, 137(1), 202-8. https://doi.org/10.1039/c1an15629b
Guven B, et al. A Rapid Method for Detection of Genetically Modified Organisms Based On Magnetic Separation and Surface-enhanced Raman Scattering. Analyst. 2012 Jan 7;137(1):202-8. PubMed PMID: 22049365.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A rapid method for detection of genetically modified organisms based on magnetic separation and surface-enhanced Raman scattering. AU - Guven,Burcu, AU - Boyacı,İsmail Hakkı, AU - Tamer,Ugur, AU - Çalık,Pınar, Y1 - 2011/11/03/ PY - 2011/11/4/entrez PY - 2011/11/4/pubmed PY - 2012/4/18/medline SP - 202 EP - 8 JF - The Analyst JO - Analyst VL - 137 IS - 1 N2 - In this study, a new method combining magnetic separation (MS) and surface-enhanced Raman scattering (SERS) was developed to detect genetically modified organisms (GMOs). An oligonucleotide probe which is specific for 35 S DNA target was immobilized onto gold coated magnetic nanospheres to form oligonucleotide-coated nanoparticles. A self assembled monolayer was formed on gold nanorods using 5,5'-dithiobis (2-nitrobenzoic acid) (DTNB) and the second probe of the 35 S DNA target was immobilized on the activated nanorod surfaces. Probes on the nanoparticles were hybridized with the target oligonucleotide. Optimization parameters for hybridization were investigated by high performance liquid chromatography. Optimum hybridization parameters were determined as: 4 μM probe concentration, 20 min immobilization time, 30 min hybridization time, 55 °C hybridization temperature, 750 mM buffer salt concentration and pH: 7.4. Quantification of the target concentration was performed via SERS spectra of DTNB on the nanorods. The correlation between the target concentration and the SERS signal was found to be linear within the range of 25-100 nM. The analyses were performed with only one hybridization step in 40 min. Real sample analysis was conducted using Bt-176 maize sample. The results showed that the developed MS-SERS assay is capable of detecting GMOs in a rapid and selective manner. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/22049365/A_rapid_method_for_detection_of_genetically_modified_organisms_based_on_magnetic_separation_and_surface_enhanced_Raman_scattering_ L2 - https://doi.org/10.1039/c1an15629b DB - PRIME DP - Unbound Medicine ER -