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Preparative separation of methylswertianin, swerchirin and decussatin from the Tibetan medicinal plant Swertia mussotii using high-speed counter-current chromatography.
Phytochem Anal. 2012 Jul-Aug; 23(4):332-6.PA

Abstract

INTRODUCTION

Xanthones, the primary constituents of Swertia mussotii, are known to possess a variety of biological activities, including anti-depressant, anti-leukaemic, anti-tumour, anti-tubercular, choleretic, diuretic, anti-microbial, anti-fungal, anti-inflammatory, anti-viral, cardiotonic and hypoglycemic properties. However, high performance, environmentally friendly methods for isolating and purifying xanthones from S. mussotii are not currently available.

OBJECTIVE

To develop a high performance and environmentally friendly method for the preparative separation of xanthones methylswertianin, swerchirin and decussatin from S. mussotii using high-speed counter-current chromatography (HSCCC).

METHODOLOGY

A solvent system composed of n-hexane:ethyl acatate:methanol:water (5:5:10:4, v/v/v/v) was developed for the separation method. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5 mL/min, a rotation speed of 800 rpm and a temperature of 25 °C.

RESULTS

Using the described method, 8 mg of methylswertianin, 21 mg of swerchirin and 11 mg of decussatin with purities of over 98% could be isolated from a 150 mg crude sample. They were identified by [1]H-NMR and [13]C-NMR analysis.

CONCLUSION

Three xanthones in Swertia mussotii could be systematically isolated and purified using HSCCC.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Jia J
Key Laboratory of Adaptation and Evolution of Plateau Biota, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining 810001, PR China.
Chen T
No affiliation info available
Wang P
No affiliation info available
Chen G
No affiliation info available
You J
No affiliation info available
Liu Y
No affiliation info available
Li Y
No affiliation info available

MeSH

AcetatesCountercurrent DistributionHexanesMagnetic Resonance SpectroscopyMethanolMolecular StructurePlant ExtractsPlants, MedicinalSolventsSwertiaTemperatureWaterXanthones

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22052665

Citation

Jia, Jing, et al. "Preparative Separation of Methylswertianin, Swerchirin and Decussatin From the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography." Phytochemical Analysis : PCA, vol. 23, no. 4, 2012, pp. 332-6.
Jia J, Chen T, Wang P, et al. Preparative separation of methylswertianin, swerchirin and decussatin from the Tibetan medicinal plant Swertia mussotii using high-speed counter-current chromatography. Phytochem Anal. 2012;23(4):332-6.
Jia, J., Chen, T., Wang, P., Chen, G., You, J., Liu, Y., & Li, Y. (2012). Preparative separation of methylswertianin, swerchirin and decussatin from the Tibetan medicinal plant Swertia mussotii using high-speed counter-current chromatography. Phytochemical Analysis : PCA, 23(4), 332-6. https://doi.org/10.1002/pca.1362
Jia J, et al. Preparative Separation of Methylswertianin, Swerchirin and Decussatin From the Tibetan Medicinal Plant Swertia Mussotii Using High-speed Counter-current Chromatography. Phytochem Anal. 2012 Jul-Aug;23(4):332-6. PubMed PMID: 22052665.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Preparative separation of methylswertianin, swerchirin and decussatin from the Tibetan medicinal plant Swertia mussotii using high-speed counter-current chromatography. AU - Jia,Jing, AU - Chen,Tao, AU - Wang,Ping, AU - Chen,Guichen, AU - You,Jinmao, AU - Liu,Yongjun, AU - Li,Yulin, Y1 - 2011/11/03/ PY - 2011/02/28/received PY - 2011/08/19/revised PY - 2011/08/29/accepted PY - 2011/11/5/entrez PY - 2011/11/5/pubmed PY - 2012/10/26/medline SP - 332 EP - 6 JF - Phytochemical analysis : PCA JO - Phytochem Anal VL - 23 IS - 4 N2 - INTRODUCTION: Xanthones, the primary constituents of Swertia mussotii, are known to possess a variety of biological activities, including anti-depressant, anti-leukaemic, anti-tumour, anti-tubercular, choleretic, diuretic, anti-microbial, anti-fungal, anti-inflammatory, anti-viral, cardiotonic and hypoglycemic properties. However, high performance, environmentally friendly methods for isolating and purifying xanthones from S. mussotii are not currently available. OBJECTIVE: To develop a high performance and environmentally friendly method for the preparative separation of xanthones methylswertianin, swerchirin and decussatin from S. mussotii using high-speed counter-current chromatography (HSCCC). METHODOLOGY: A solvent system composed of n-hexane:ethyl acatate:methanol:water (5:5:10:4, v/v/v/v) was developed for the separation method. The upper phase was used as the stationary phase, and the lower phase was used as the mobile phase at a flow rate of 1.5 mL/min, a rotation speed of 800 rpm and a temperature of 25 °C. RESULTS: Using the described method, 8 mg of methylswertianin, 21 mg of swerchirin and 11 mg of decussatin with purities of over 98% could be isolated from a 150 mg crude sample. They were identified by [1]H-NMR and [13]C-NMR analysis. CONCLUSION: Three xanthones in Swertia mussotii could be systematically isolated and purified using HSCCC. SN - 1099-1565 UR - https://www.unboundmedicine.com/medline/citation/22052665/Preparative_separation_of_methylswertianin_swerchirin_and_decussatin_from_the_Tibetan_medicinal_plant_Swertia_mussotii_using_high_speed_counter_current_chromatography_ DB - PRIME DP - Unbound Medicine ER -